Results 71 to 80 of about 144,685 (260)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Soil Aggregate Stability Under Freeze–Thaw Cycles in Mollisols as Evidenced by ¹⁵N Distribution

Agronomy
Freeze–thaw cycles (FTCs) influence soil nitrogen (N) dynamics and soil aggregate stability. However, the driving mechanism affecting aggregate stability from the combined perspective of N components and N distribution by 15N tracing technology in both ...
Yao Wang, Meng Zhou, Xiaoguang Jiao, Liangqian Ma, He Yu, Yimin Chen, Yueyu Sui   +6 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Land-use shapes the composition and stability of soil water-stable aggregates in a plateau agro-pastoral ecotone [PDF]

PeerJ
Background The northeastern Tibetan Plateau is a typical agro-pastoral ecotone that experiences frequent land-use changes. Maintaining stable soil aggregates in this region is key to protecting the environment and supporting food production on the ...
Ling Bai, Wei Wang, Yujuan Mo, Xubin Zhang, Jianchao Fu, Longfei Shu, Zining Yue, Peng Liu, Jian Hao, Xiang Liu, Deming Zhang   +10 more
doaj   +2 more sources

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

On the stability of utilitarian aggregation

Theory and Decision
Abstract In the context of aggregating von Neumann-Morgenstern utilities, we show that bounded violations of the Pareto conditions characterize aggregation rules that are approximately utilitarian. When a single utility function is intended to represent the preference judgments of a group of individuals and the Pareto principles are ...
openaire   +2 more sources

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Cement-phosphogypsum stabilized aggregate interlocking road base material: preparation, application and environmental impact

Journal of Infrastructure Preservation and Resilience
For the extensive and cost-effective utilization of phosphogypsum in highway engineering, a cement-phosphogypsum stabilized coarse aggregate interlocking road base material is developed in this study.
Zhicheng Zhao, Weiguo Shen, Gelong Xu, Xiaodang Peng, Guiming Wang, Shiyong Xu, Shuzhao Luo, Qian Ma, Qisheng Wu, Xuetian Yu   +9 more
doaj   +1 more source

Quantifying the contribution of the root system of alpine vegetation in the soil aggregate stability of moraine

International Soil and Water Conservation Research, 2017
One fifth of the world's population is living in mountains or in their surrounding areas. This anthropogenic pressure continues to grow with the increasing number of settlements, especially in areas connected to touristic activities, such as the Italian ...
Csilla Hudek, Silvia Stanchi, Michele D’Amico, Michele Freppaz   +3 more
doaj   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source