Results 151 to 160 of about 152,322 (388)

Agrobacterium tumefaciens Gene Transfer: How a Plant Pathogen Hacks the Nuclei of Plant and Nonplant Organisms.

Phytopathology, 2015
Agrobacterium species are soilborne gram-negative bacteria exhibiting predominantly a saprophytic lifestyle. Only a few of these species are capable of parasitic growth on plants, causing either hairy root or crown gall diseases.
S. Bourras, T. Rouxel, M. Meyer
semanticscholar   +1 more source

Global identification of key genes for pollen germination in rice through high‐throughput screening and gene editing

Journal of Integrative Plant Biology, EarlyView.
A systematic examination of 107 T‐DNA insertional lines targeting 105 genes preferentially expressed in late pollen development identified 42 lines exhibiting significant deviations from expected Mendelian segregation ratios, suggesting a potential involvement in pollen viability and germination.
Eui‐Jung Kim, Woo‐Jong Hong, Yu‐Jin Kim, Eun Young Kim, Sang Dae Yun, Sunok Moon, Su‐Kyoung Lee, Soon Ki Park, Ki‐Hong Jung   +8 more
wiley   +1 more source

Discovery of the Agrobacterium growth inhibition sequence in virus and its application to recombinant clone screening

AMB Express, 2019
Infectious clone vectors used widely in genetic research. While constructing soybean mosaic virus (SMV) clone vectors, we found that transformed Agrobacterium grew significantly different depending on the viral strains used.
Jinlong Yin, Hui Liu, Wenyang Xiang, Tongtong Jin, Dongquan Guo, Liqun Wang, Haijian Zhi   +6 more
doaj   +1 more source

Assessment of RNAi-induced silencing in banana (Musa spp.) [PDF]

, 2015
In plants, RNA- based gene silencing mediated by small RNAs functions at the transcriptional or post-transcriptional level to negatively regulate target genes, repetitive sequences, viral RNAs and/or transposon elements.
Cammue, B.P.A., Coninck, B. de, Dang Thi Tuong Vi, Henry, I., Remy, S., Swennen, Rony L., Windelinckx, S.   +6 more
core   +1 more source

The road less taken: Dihydroflavonol 4‐reductase inactivation and delphinidin anthocyanin loss underpins a natural intraspecific flower colour variation

Molecular Ecology, EarlyView.
Abstract Visual cues are of critical importance for the attraction of animal pollinators, however, little is known about the molecular mechanisms underpinning intraspecific floral colour variation. Here, we combined comparative spectral analysis, targeted metabolite profiling, multi‐tissue transcriptomics, differential gene expression, sequence ...
Darren C. J. Wong, Zemin Wang, James Perkins, Xin Jin, Grace Emma Marsh, Emma Grace John, Rod Peakall   +6 more
wiley   +1 more source

Agrobacterium tumefaciens responses to plant-derived signaling molecules

Frontiers in Plant Science, 2014
As a special phytopathogen, Agrobacterium tumefaciens infects a wide range of plant hosts and causes plant tumors also known as crown galls. The complexity of Agrobacterium-plant interaction has been studied for several decades.
Sujatha eSubramoni, Naeem eNathoo, Eugene eKlimov, Ze-Chun eYuan, Ze-Chun eYuan   +4 more
doaj   +1 more source

On the isolation of TI-plasmid from Agrobacterium tumefaciens [PDF]

, 1976
An efficient lysis method for Agrobacterium cells was developed, which allows a reproducible isolation of the tumor inducing (TI)-plasmid. The lysis method is based on the sensitivity of this bacterium to incubation with lysozyme, n-dodecylamine,EDTA ...
Dons, Johannes JM, Krol, AJM, Ledeboer, Adrianus M, Schell, Jeff, Schilperoort, Robert A, Spier, F, Van Larebeke, Nicolas, Zaenen, Ivo   +7 more
core   +2 more sources

A novel and fully scalable Agrobacterium spray-based process for manufacturing cellulases and other cost-sensitive proteins in plants.

Plant Biotechnology Journal, 2015
Transient transfection of plants by vacuum infiltration of Agrobacterium vectors represents the state of the art in plant-based protein manufacturing; however, the complexity and cost of this approach restrict it to pharmaceutical proteins.
S. Hahn, A. Giritch, D. Bartels, L. Bortesi, Y. Gleba   +4 more
semanticscholar   +1 more source

SacB-SacR gene cassette as the negative selection marker to suppress Agrobacterium overgrowth in Agrobacterium-mediated plant transformation

Frontiers in Molecular Biosciences, 2016
Agrobacterium overgrowth is a common problem in Agrobacterium-mediated plant transfor-mation. To suppress the Agrobacterium overgrowth, various antibiotics have been used during plant tissue culture steps.
Yiming Liu, Jiamin Miao, Sy Traore, Danyu Kong, Yi Liu, Xunzhong Zhang, Zachary Nimchuk, Zongrang Liu, Bingyu Zhao   +8 more
doaj   +1 more source

The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species

Plant Methods, 2009
BackgroundPlant genome sequencing has resulted in the identification of a large number of uncharacterized genes. To investigate these unknown gene functions, several transient transformation systems have been developed as quick and convenient ...
Jian-Feng Li, Eunsook Park, A. V. von Arnim, A. Nebenführ   +3 more
semanticscholar   +1 more source