Results 181 to 190 of about 166,091 (383)

Electroporation of Agrobacterium v1

open access: yes, 2017
A quick guide on how to electroporate your plasmid of interest into electrocompetent Agrobacterium tumefaciens cells.
openaire   +2 more sources

Agrobacterium-mediated Transformation of Strawberry

open access: yesBIO-PROTOCOL, 2014
Traditional breeding for improvement of strawberry (Fragaria x ananassa) is difficult because strawberry is an octoploid, hybrid species. Genetic modification of strawberry would though be a promising alternative for obtaining the desired improvements in existing elite strawberry cultivars (Schaart et al., 2011).
openaire   +4 more sources

Identification and characterization of a cucumber accession resistant to tomato leaf curl New Delhi virus

open access: yesAnnals of Applied Biology, EarlyView.
Tomato leaf curl New Delhi virus (ToLCNDV) causes significant yield and fruit quality losses in cucurbit crops, particularly in the Mediterranean region. We identified a new source of resistance to ToLCNDV in cucumber (accession ABS.PE.045) and mapped resistance to four genomic intervals in chromosomes 1, 2, 6, and 7.
Livia Donaire   +5 more
wiley   +1 more source

Detection of mutations induced by CRISPR/Cas9 in cabbage protoplasts using qPCR and dPCR

open access: yesAnnals of Applied Biology, EarlyView.
This study evaluated two PCR‐based methods—qPCR and dPCR—for detecting CRISPR‐induced mutations in transformed cabbage protoplasts. Both methods distinguished edited from wild‐type protoplasts, offering rapid and cost‐efficient alternatives for mutation detection, with dPCR providing higher sensitivity and closer agreement with NGS results.
Ester Stajič   +2 more
wiley   +1 more source

The importin‐alpha superfamily engages in ethylene signaling by shuttling ETHYLENE INSENSITIVE 2 from the endoplasmic reticulum to the nucleus

open access: yesThe FEBS Journal, EarlyView.
The plant hormone ethylene regulates plant growth, ripening, senescence, and stress responses. The hormonal signal transmission, from receptors at the ER membrane to the transcriptional regulators in the nucleus, is still not completely understood.
Fabian Wynen   +7 more
wiley   +1 more source

The bZIP3‐AS1 complex promotes CONSTANS‐induced FLOWERING LOCUS T activation in a daylength‐dependent manner

open access: yesJournal of Integrative Plant Biology, EarlyView.
The Arabidopsis bZIP3 protein works in conjunction with ASYMMETRIC LEAVES1 to facilitate the function of the CONSTANS protein in activating FLOWERING LOCUS T expression at the end of the day in a day length‐dependent manner. ABSTRACT Plants monitor daylength to synchronize their flowering time with their surroundings and thus maximize reproductive ...
Wonbok Lee   +10 more
wiley   +1 more source

Utilization of Octopine and Nopaline by Agrobacterium [PDF]

open access: bronze, 1973
James A. Lippincott   +2 more
openalex   +1 more source

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