Results 241 to 250 of about 166,091 (383)

Molecular changes in agroinfiltrated leaves of Nicotiana benthamiana expressing suppressor of silencing P19 and coronavirus‐like particles

open access: yesPlant Biotechnology Journal, EarlyView.
Summary The production of coronavirus disease 2019 vaccines can be achieved by transient expression of the spike (S) protein of severe acute respiratory syndrome coronavirus 2 in agroinfiltrated leaves of Nicotiana benthamiana. Relying on bacterial vector Agrobacterium tumefaciens, this process is favoured by co‐expression of viral silencing suppressor
Louis‐Philippe Hamel   +9 more
wiley   +1 more source

Localising enzymes to biomolecular condensates increases their accumulation and benefits engineered metabolic pathway performance in Nicotiana benthamiana

open access: yesPlant Biotechnology Journal, EarlyView.
Summary The establishment of Nicotiana benthamiana as a robust biofactory is complicated by issues such as product toxicity and proteolytic degradation of target proteins/introduced enzymes. Here we investigate whether biomolecular condensates can be used to address these problems. We engineered biomolecular condensates in N.
Anya L. Lindström Battle   +3 more
wiley   +1 more source

Rhizobium rhizogenes A4‐derived strains mediate hyper‐efficient transient gene expression in Nicotiana benthamiana and other solanaceous plants

open access: yesPlant Biotechnology Journal, EarlyView.
Summary Agroinfiltration, a method utilizing agrobacteria to transfer DNA into plant cells, is widely used for transient gene expression in plants. Besides the commonly used Agrobacterium strains, Rhizobium rhizogenes can also introduce foreign DNA into host plants for gene expression. While many R. rhizogenes strains have been known for inducing hairy
Juan Carlos Lopez‐Agudelo   +11 more
wiley   +1 more source

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