Results 21 to 30 of about 66,578 (299)

Transcriptome architecture of the three main lineages of agrobacteria

mSystems, 2023
Agrobacteria are a diverse, polyphyletic group of prokaryotes with multipartite genomes capable of transferring DNA into the genomes of host plants, making them an essential tool in plant biotechnology.
Lucas Waldburger, Mitchell G. Thompson, Alexandra J. Weisberg, Namil Lee, Jeff H. Chang, Jay D. Keasling, Patrick M. Shih   +6 more
doaj   +1 more source

DNA-free gene editing in plants: a brief overview

Biotechnology & Biotechnological Equipment, 2021
The conversion of bacterial CRISPR/Cas defense system into a simple and efficient tool for genome manipulations brought experimental biology into new dimensions.
Tsveta Tsanova, Lidia Stefanova, Lora Topalova, Atanas Atanasov, Ivelin Pantchev   +4 more
doaj   +1 more source

Development of an Efficient Agrobacterium Mediated Transformation Protocol for Sri Lankan Rice Variety - Bg 250

Tropical Agricultural Research, 2011
Experiments were conducted to establish an efficient and simple protocol for regeneration and Agrobacterium mediated genetic transformation of an agronomically important indica rice variety Bg 250.
RMLK Ratnayake, GHCM Hettiarachchi
doaj   +1 more source

Comparative Analysis of Chrysanthemum Stunt Viroid Accumulation and Movement in Two Chrysanthemum (Chrysanthemum morifolium) Cultivars with Differential Susceptibility to the Viroid Infection

Frontiers in Plant Science, 2017
Chrysanthemum stunt viroid (CSVd) was inoculated into two chrysanthemum (Chrysanthemum morifolium) cultivars, the CSVd-susceptible cultivar Piato and the CSVd-resistant cultivar Mari Kazaguruma.
Tomoyuki Nabeshima, Motoaki Doi, Munetaka Hosokawa   +2 more
doaj   +1 more source

Transformation of HBsAg (hepatitis B surface antigen) gene into tomato mediated by Agrobacterium tumefaciens

Czech Journal of Genetics and Plant Breeding, 2011
The plant expression vector pBRSAg was constructed as suitable for transformation via Agrobacterium-mediated approach. It contains all elements for plant expression, such as CaMV 35S promoter, both left and right border sequence for transferred DNA (T ...
Tian Li, Jing Kuan Sun, Zhao Hua Lu, Qing Liu   +3 more
doaj   +1 more source

Regeneration and Agrobacterium-mediated transformation of japonica rice varieties developed for a cold region

Czech Journal of Genetics and Plant Breeding, 2018
So far, a large number of transformation systems have been established for japonica rice, but only a few have been reported for cold-region varieties. In our study, we established highly efficient tissue culture systems for two cold-region rice cultivars,
Mingfang FENG, Jing CANG, Junhong WANG, Jian SUN, Jing YU, Qinghua XU, Da ZHANG, Ning YANG, Qiuwei LU, Yan LV   +9 more
doaj   +1 more source

Tissue culture-based Agrobacterium-mediated and in planta transformation methods

Czech Journal of Genetics and Plant Breeding, 2017
Gene transformation can be done in direct and indirect (Agrobacterium-mediated) ways. The most efficient method of gene transformation to date is Agrobacterium-mediated method.
Mohsen NIAZIAN, Seyed Ahmad SADAT NOORI, Petr GALUSZKA, Seyed Mohammad Mahdi MORTAZAVIAN   +3 more
doaj   +1 more source

A highly efficient genetic transformation system for broccoli and subcellular localization

Frontiers in Plant Science, 2023
IntroductionAgrobacterium-mediated genetic transformation has been widely used for the identification of functional genes and regulatory and developmental mechanisms in plants.
Yongyu Zhao, Dongxu Yang, Yumei Liu, Fengqing Han, Zhansheng Li   +4 more
doaj   +1 more source

Transfection in Agrobacterium tumefaciens [PDF]

Journal of Virology, 1972
Intact cells of Agrobacterium tumefaciens were examined for ability to take up biologically active LR-4 phage deoxyribonucleic acid (DNA) from the surrounding medium. DNA incorporation as measured by subsequent plaque formation (transfection) failed to occur when the bacteria were grown in defined minimal salts ...
Victor J. Milani, Gary T. Heberlein
openaire   +3 more sources

Transformation And Light Inducible Expression of cry1Ab Gene in Oilseed Rape (Brassica napus L.) [PDF]

Journal of Sciences, Islamic Republic of Iran, 2016
Rapeseed (Brassica napus L.) is the third most important oil crop in global productions. One of the major limiting factors for oilseed rape production is lepidopteran pests of the Brassicaceae family.
H. Rahnama, M. Sheykhhasan
doaj