GENETICALLY MODIFIED FRUITS: THE SOLUTION OR A PROBLEM? [GENETICKY MODIFIKOVANÉ OVOCE: ŘEŠENÍ NEBO PROBLÉM?] [PDF]
Vědecké Práce OvocnářskéGenetically modified (GM) fruit crops breeding began in the 1990s and brought several successful GM varieties. The first successful GM fruit tree was a transgenic papaya, which saved its growing industry in Hawaii.
Michaela Burešová
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Improved genome of Agrobacterium radiobacter type strain provides new taxonomic insight into Agrobacterium genomospecies 4 [PDF]
PeerJ, 2019 The reported Agrobacterium radiobacter DSM 30174T genome is highly fragmented, hindering robust comparative genomics and genome-based taxonomic analysis. We re-sequenced the Agrobacterium radiobacter type strain, generating a dramatically improved genome
Han Ming Gan +2 more
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Oriental lily hybrids engineered to resist aphid attack [PDF]
, 2009 Establishing in vitro bulb scale cultures of lily cultivars followed by callusing and regeneration after gene transfer was found to be not very successful in our hands, except for ‘Snow Queen’.
Chang, L. +4 more
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Actin Cytoskeleton and Golgi Involvement in Barley stripe mosaic virus Movement and Cell Wall Localization of Triple Gene Block Proteins. [PDF]
, 2013 Barley stripe mosaic virus (BSMV) induces massive actin filament thickening at the infection front of infected Nicotiana benthamiana leaves. To determine the mechanisms leading to actin remodeling, fluorescent protein fusions of the BSMV triple gene ...
Bae, Hanhong +10 more
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Ethylene Response of Plum ACC Synthase 1 (ACS1) Promoter is Mediated through the Binding Site of Abscisic Acid Insensitive 5 (ABI5). [PDF]
, 2019 The enzyme 1-amino-cyclopropane-1-carboxylic acid synthase (ACS) participates in the ethylene biosynthesis pathways and it is tightly regulated transcriptionally and post-translationally.
Blumwald, Eduardo +7 more
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On the isolation of TI-plasmid from Agrobacterium tumefaciens [PDF]
, 1976 An efficient lysis method for Agrobacterium cells was developed, which allows a reproducible isolation of the tumor inducing (TI)-plasmid. The lysis method is based on the sensitivity of this bacterium to incubation with lysozyme, n-dodecylamine,EDTA ...
Dons, Johannes JM +7 more
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A universal primer for isolation of fragments of a gene encoding phytoene desaturase for use in virus-induced gene silencing (VIGS) studies [PDF]
, 2010 We have been using Virus-Induced Gene Silencing (VIGS) to test the function of genes that are candidates for involvement in floral senescence. Although VIGS is a powerful tool for assaying the effects of gene silencing in plants, relatively few taxa have
Cai-Zhong, J. +4 more
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Genetic transformation of barley: limiting factors
Biologia Plantarum, 2011 This review summarizes main difficulties involved in barley (Hordeum vulgare L.) transformation. The most commonly used procedures for genetic transformation in barley are Agrobacterium tumefaciens and particle bombardment mediated methods.
Š. Vyroubalová +3 more
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Agrobacterium-mediated barley transformation. [PDF]
, 2008 More than ten years have passed since the first successful Agrobacterium-mediated barley transformation experiment, however it is still quite challenging to establish a stably functioning agroinfiltration protocol.
Csóti, László +2 more
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Targeted genome modifications in soybean with CRISPR/Cas9 [PDF]
, 2015 Background: The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms.
Jacobs, Thomas +3 more
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