A simple and efficient protocol for generating transgenic hairy roots using Agrobacterium rhizogenes. [PDF]
For decades, Agrobacterium rhizogenes (now Rhizobium rhizogenes), the causative agent of hairy root disease, has been harnessed as an interkingdom DNA delivery tool for generating transgenic hairy roots on a wide variety of plants.
Ferguson S +7 more
europepmc +2 more sources
Stable transformation mediated by <i>Agrobacterium tumefaciens</i> in Jonquil is better than <i>Agrobacterium rhizogenes</i>. [PDF]
Agrobacterium tumefaciens-mediated genetic transformation has become an important method to study gene function and create new germplasm in plants. The classic genetic transformation process is time-consuming and laborious.
Yu W, Tian H, Chen Y, Lyu S, Fan Y.
europepmc +2 more sources
Altered carbon status in Glycine max hairy roots induced by Agrobacterium rhizogenes. [PDF]
Plants fix CO2 into carbohydrates through photosynthesis, and various organisms interact with plants to obtain carbohydrates. Agrobacterium rhizogenes is a soil bacterium known as a plant pathogen that induces hairy root disease. Through A.
Okamoto S, Ueki Y.
europepmc +2 more sources
<i>Agrobacterium rhizogenes</i>-Mediated Transformation for Generation of Composite Sugar Beet with Transgenic Adventitious Roots. [PDF]
Sugar beet (Beta vulgaris L.), a biennial sugar crop, provides about 16% of the world’s sucrose production. PEG and Agrobacterium tumefaciens-mediated transformation have been established for sugar beet.
Sun Y +7 more
europepmc +2 more sources
Agrobacterium rhizogenes: paving the road to research and breeding for woody plants. [PDF]
Woody plants play a vital role in global ecosystems and serve as valuable resources for various industries and human needs. While many woody plant genomes have been fully sequenced, gene function research and biotechnological breeding advances have ...
Ying W +12 more
europepmc +2 more sources
Insight in the transcriptome data of hairy root disease-causing bacterium-Agrobacterium rhizogenes. [PDF]
Agrobacterium rhizogenes induce the production of the hairy root through the transformation of plant genomes. In this article, we executed the transcriptome of A. rhizogenes through RNA-sequencing. RNA-sequencing of A. rhizogenes generated a total of 2.6
Yadav A +4 more
europepmc +2 more sources
A robust in-vitro and ex-vitro Agrobacterium rhizogenes-mediated hairy root transformation system in mungbean for efficient visual screening of transformants using the RUBY reporter. [PDF]
Background Mungbean is one of the most economically important grain legume crops in Asia. Functional genomics studies in mungbean are necessary to understand the molecular mechanisms behind agronomic traits, to advance the crop improvement. However, this
Kumar S +3 more
europepmc +2 more sources
Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation for gene functional and gene editing analysis in soybean. [PDF]
Background Agrobacterium-mediated genetic transformation is a widely used and efficient technique for gene functional research in crop breeding and plant biology.
Cheng Y +6 more
europepmc +2 more sources
Agrobacterium rhizogenes-mediated transformation of Pisum sativum L. roots as a tool for studying the mycorrhizal and root nodule symbioses. [PDF]
In this study, we demonstrated the successful transformation of two pea (Pisum sativum L.) cultivars using Agrobacterium rhizogenes, whereby transgenic roots in the resulting composite plants showed expression of the gene encoding the green fluorescent ...
Leppyanen IV, Kirienko AN, Dolgikh EA.
europepmc +3 more sources
Transformation and Detection of Soybean Hairy Roots
Agrobacterium rhizogenes is a soil bacteria with extensive infectivity, which can infect almost all dicotyledonous plants and a few monocotyledonous plants to induce root nodules.
Xing Xu +9 more
doaj +1 more source

