Results 1 to 10 of about 55,695 (299)

A Rapid and Visual Soybean Hairy Root Transformation Protocol Using the RUBY Reporter [PDF]

Bio-Protocol
Agrobacterium rhizogenes–mediated hairy root transformation provides a rapid platform for gene function analysis prior to stable whole-plant transformation.
Zhirui Zhang, Qiushi Wang, Yu Geng, Jiuhai Zhao   +3 more
doaj   +2 more sources

Injection-based hairy root induction and plant regeneration techniques in Brassicaceae [PDF]

Plant Methods
Background Hairy roots constitute a valuable tissue culture system for species that are difficult to propagate through conventional seed-based methods. Moreover, the generation of transgenic plants derived from hairy roots can be facilitated by employing
Veronika Jedličková, Marie Štefková, Terezie Mandáková, Juan Francisco Sánchez López, Marek Sedláček, Martin A. Lysak, Hélène S. Robert   +6 more
doaj   +2 more sources

Compounds from medicinal plants produced in hairy root and transgenic hairy root cultures: a review. [PDF]

PeerJ
Background Plants produce a wide variety of molecules, and some of them are of medicinal importance. Biotechnological systems such as suspended cells and hairy roots (HR) are used to produce plant compounds in a stable and controlled manner.
Urquiza-López A, Bazaldúa C, Cardoso-Taketa AT, Villarreal ML.   +3 more
europepmc   +3 more sources

Reprogramming Hairy Root Cultures: A Synthetic Biology Framework for Precision Metabolite Biosynthesis [PDF]

Plants
Hairy root cultures induced by Agrobacterium rhizogenes (Rhizobium rhizogenes) provide a sustainable approach to meet the growing demand for economically valuable plant-derived compounds in the face of depleting natural resources.
Chang Liu, Naveed Ahmad, Ye Tao, Hamad Hussain, Yue Chang, Abdul Wakeel Umar, Xiuming Liu   +6 more
doaj   +2 more sources

Elicitor-enhanced steroidal sapogenin accumulation in hairy root cultures of Trigonella foenum-graecum [PDF]

Scientific Reports
In current work, we studied hairy root induction in Trigonella foenum graecum, which is an important medicinal plant, and examined the impact of different elicitors on some phytochemical characteristics and metabolites production in hairy root cultures ...
Poorak Rezazadehfar, Maryam Rezayian, Vahid Niknam, Masoud Mirmasoumi   +3 more
doaj   +2 more sources

Metabolic engineering of hairy root cultures in Beta vulgaris for enhanced production of vanillin, 4-hydroxybenzoic acid, and vanillyl alcohol [PDF]

Frontiers in Bioengineering and Biotechnology
The flavor of vanilla is a complex blend of compounds, with vanillin as the most prominent, along with vanillyl alcohol and 4-hydroxybenzoic acid. Natural vanillin extracted from vanilla beans is expensive, so researchers use heterologous synthesis to ...
Zakir Husain, Zafar Iqbal Warsi, Sana Khan, Ganesan Mahendran, Shama Afroz, Ashish Chandran, Praveen Kumar Kashyap, Kahkashan Khatoon, Gazala Parween, Sudeep Tandon, Laiq Ur Rahman   +10 more
doaj   +2 more sources

Hairy CRISPR: Genome Editing in Plants Using Hairy Root Transformation [PDF]

Plants, 2021
CRISPR/Cas-mediated genome editing is a powerful tool of plant functional genomics. Hairy root transformation is a rapid and convenient approach for obtaining transgenic roots. When combined, these techniques represent a fast and effective means of studying gene function.
Alexey S. Kiryushkin, Elena L. Ilina, Elizaveta D. Guseva, Katharina Pawlowski, Kirill N. Demchenko   +4 more
openaire   +3 more sources

First Report of Agrobacterium rhizogenes-induced Hairy Root Formation in Selaginella bryopteris: a Pteridophyte Recalcitrant to Genetic Transformation [PDF]

Brazilian Archives of Biology and Technology, 2020
we report A. rhizogenes-induced hairy root formation in S. bryopteris, a medicinally and commercially important plant. A. rhizogenes strain LBA1334 co-cultivated with explants (root, rhizophore, stem portion near the root, and stem with intact fronds ...
Ravi Shankar Singh, Vikash Kumar Jha, Tirthartha Chattopadhyay, Ujjwal Kumar, Devanand Pralhad Fulzele, Prabhash Kumar Singh   +5 more
doaj   +1 more source

Assessment of hairy roots induction in Echium khuzistanicum by different strains of Agrobacterium rhizogenes [PDF]

مجله بیوتکنولوژی کشاورزی, 2020
Objective In this study for the first time, different strains of Agrobacterium rhizogenes, co-cultivation times, and concentrations of acetosyringon in leaf explants of Echium khuzistanicum were examined to produce hairy roots.
samira mohammadi, Raheem Haddad, Vahid Mohammadi   +2 more
doaj   +1 more source

Targeted metabolomics unveil alteration in accumulation and root exudation of flavonoids as a response to interspecific competition

Journal of Plant Interactions, 2021
This study aimed to elucidate the chemical response of hairy vetch (Vicia villosa Roth) to interspecific competition by rye (Secale cereale L.). Hairy vetch plants were grown as target plants in a mixture with different densities of rye plants (0, 1, 2 ...
Hossein Hazrati, Inge S. Fomsgaard, Per Kudsk   +2 more
doaj   +1 more source