A Rapid and Visual Soybean Hairy Root Transformation Protocol Using the RUBY Reporter [PDF]
Agrobacterium rhizogenes–mediated hairy root transformation provides a rapid platform for gene function analysis prior to stable whole-plant transformation.
Zhirui Zhang +3 more
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Injection-based hairy root induction and plant regeneration techniques in Brassicaceae [PDF]
Background Hairy roots constitute a valuable tissue culture system for species that are difficult to propagate through conventional seed-based methods. Moreover, the generation of transgenic plants derived from hairy roots can be facilitated by employing
Veronika Jedličková +6 more
doaj +2 more sources
Reprogramming Hairy Root Cultures: A Synthetic Biology Framework for Precision Metabolite Biosynthesis [PDF]
Hairy root cultures induced by Agrobacterium rhizogenes (Rhizobium rhizogenes) provide a sustainable approach to meet the growing demand for economically valuable plant-derived compounds in the face of depleting natural resources.
Chang Liu +6 more
doaj +2 more sources
Elicitor-enhanced steroidal sapogenin accumulation in hairy root cultures of Trigonella foenum-graecum [PDF]
In current work, we studied hairy root induction in Trigonella foenum graecum, which is an important medicinal plant, and examined the impact of different elicitors on some phytochemical characteristics and metabolites production in hairy root cultures ...
Poorak Rezazadehfar +3 more
doaj +2 more sources
Metabolic engineering of hairy root cultures in Beta vulgaris for enhanced production of vanillin, 4-hydroxybenzoic acid, and vanillyl alcohol [PDF]
The flavor of vanilla is a complex blend of compounds, with vanillin as the most prominent, along with vanillyl alcohol and 4-hydroxybenzoic acid. Natural vanillin extracted from vanilla beans is expensive, so researchers use heterologous synthesis to ...
Zakir Husain +10 more
doaj +2 more sources
First Report of Agrobacterium rhizogenes-induced Hairy Root Formation in Selaginella bryopteris: a Pteridophyte Recalcitrant to Genetic Transformation [PDF]
we report A. rhizogenes-induced hairy root formation in S. bryopteris, a medicinally and commercially important plant. A. rhizogenes strain LBA1334 co-cultivated with explants (root, rhizophore, stem portion near the root, and stem with intact fronds ...
Ravi Shankar Singh +5 more
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Assessment of hairy roots induction in Echium khuzistanicum by different strains of Agrobacterium rhizogenes [PDF]
Objective In this study for the first time, different strains of Agrobacterium rhizogenes, co-cultivation times, and concentrations of acetosyringon in leaf explants of Echium khuzistanicum were examined to produce hairy roots.
samira mohammadi +2 more
doaj +1 more source
This study aimed to elucidate the chemical response of hairy vetch (Vicia villosa Roth) to interspecific competition by rye (Secale cereale L.). Hairy vetch plants were grown as target plants in a mixture with different densities of rye plants (0, 1, 2 ...
Hossein Hazrati +2 more
doaj +1 more source
Optimization of hairy root culture establishment in Chicory plants (Cichorium intybus) through inoculation by Agrobacterium rhizogenes [PDF]
Agrobacterium rhizogenes induced hairy roots gain of the potential of production and accumulation of plant secondary metabolites. In recent decades, many researchers have focused on the biosynthesis of valuable secondary metabolites in hairy roots ...
Sara kabirnotaj +3 more
doaj +1 more source
Effect of iron oxide nanoparticles on hairy root induction and antioxidant activity in Purslane (Portulaca oleracea) [PDF]
Objective Many secondary metabolites produced by plants are used in manufacturing drugs and new treatments, but are too difficult, expensive or impossible to prepared by chemical synthesis.
Mehdi Mohebodini, Roghayeh Fathi
doaj +1 more source

