Results 41 to 50 of about 903,495 (253)

Improving Yield of a Recombinant Biologic in a Brassica Hairy Root Manufacturing Process. [PDF]

, 2022
International audienceHairy root systems have proven to be a viable alternative for recombinant protein production. For recalcitrant proteins, maximizing the productivity of hairy root cultures is essential.
Jean‐Pierre Ele‐Ekouna, Noemi Gutierrez‐Valdes, Florian Cardon, Ele‐Ekouna, Jean‐Pierre, Gutierrez‐Valdes, Noemi, Groot, Jonas,, Marina Guillet, Camille Lemasson, Ritala, Anneli, Cardon, Florian, Ele-Ekouna, Jean-Pierre, Suvi T. Häkkinen, Groot, Jonas, De, Gutierrez-Valdes, Noemi, Häkkinen, Suvi T.; id_orcid, Guillet, Marina, Jonas de Groot, Lemasson, Camille, De Groot, Jonas, Anneli Ritala, Häkkinen, Suvi T.   +20 more
core   +1 more source

Targeting CD300a Signaling With a Phosphatidylserine‐Presenting Hydrogel Rescues Macrophage Dysfunction and Accelerates Diabetic Wound Healing

Advanced Healthcare Materials, EarlyView.
Schematic illustration of the proposed mechanism: PEG/RGD‐PSLs mimic apoptotic cells to engage PS receptors (notably CD300a), transducing an inhibitory signal that suppresses the MyD88/NF‐κB pathway, leading to global anti‐inflammatory and pro‐reparative effects.
Lele Wu, Xuetong Hao, Yang Zou, Xinglin Chen, Junyan Kong, Chunning Gu, Wenjie Ma, Zhongjun Yang, Huifen Liu, Mengmeng Liu, Xin Tong   +10 more
wiley   +1 more source

Advancing Human Skin Equivalents: The Crucial Role of Neurovascular Integration

Advanced Healthcare Materials, EarlyView.
This review discusses the importance of integrating vascular and peripheral nerve systems into human skin equivalents (HSEs) to better recapitulate native skin physiology. Recent advances in vascularized, innervated, and neurovascularized HSEs are highlighted, together with emerging bioengineering strategies, current challenges, and future ...
Hao Wu, Lorenzo Moroni, Nicolas Lebonvallet, Vincent Flacher, Paul Wieringa   +4 more
wiley   +1 more source

Self‐Healing Hydrogel‐Enabled Modular Assembly of Bilayered Skin Construct for Hair Follicle Regeneration

Advanced Healthcare Materials, EarlyView.
Self‐healing assembly of two cell‐laden hydrogel layers creates a biomimetic skin construct that restores both dermal and epidermal functions, resulting in accelerated wound healing and enhanced hair follicle regeneration in a full‐thickness skin wound model. ABSTRACT Alopecia remains a pervasive clinical challenge, largely owing to the limited ability
JaeWook Park, Su Yong Kim, MinSu Jeong, Beom Joon Kim, Shin Hyuk Kang, Won‐Gun Koh   +5 more
wiley   +1 more source

Near‐Infrared Light‐Activated Polydopamine Nanoparticles Conjugated With Transferrin Peptides for Enhanced Brain Accumulation

Advanced Healthcare Materials, EarlyView.
This study demonstrates that Tf‐PDA NPs combine a receptor‐mediated targeting with mild photothermal activation to enhance nanoparticle accumulation in the brain. NIR‐activated Tf‐PDA NPs increase BBB permeability to NPs in vitro and in vivo without inducing cytotoxicity or adverse immune responses.
Rafaela Ferrão, Susana Simões, Paulo Rodrigues‐Santos, Vitor Francisco, Akhilesh Rai, Lino Ferreira   +5 more
wiley   +1 more source

Sugar availability modulates polyisoprenoid and phytosterol profiles in Arabidopsis thaliana hairy root culture. [PDF]

, 2013
Sugars are recognized as signaling molecules regulating the biosynthesis of secondary metabolites in plants. Here, a modulatory effect of sugars on dolichol and phytosterol profiles was noted in the hairy roots of Arabidopsis thaliana.
Dydak, Marta, Swiezewska, Ewa, Kania, Magdalena, Jóźwiak, Adam, Skorupinska-Tudek, Karolina, Ples, Magdalena, Danikiewicz, Witold   +6 more
core   +1 more source

Production of hairy root cultures of lettuce (Lactuca sativa L.)

, 2014
Hairy root cultures of lettuce (Lactuca sativa L.) were obtained by inoculation of cotyledonary leaves of in vitro lettuce seedlings (cvs. Nansen and Ljubljanska ledenka) with Agrobacterium rhizogenes A4M70GUS.
Marija Petrić, Tadić, Vojin, Subotić, Angelina, Dragana Antonić, Cingel, Aleksandar, Jovan Tadić, Petrić, Marija, Trifunović-Momčilov, Milana, Antonić Reljin, Dragana, Vojin Tadić, Jovan, Tadic, Milošević, Snežana, Aleksandar Cingel, Angelina Subotić, Antonić, Dragana, Milana Trifunović, Snežana Milošević   +16 more
core   +1 more source

Bioinspired Adaptive Sensors: A Review on Current Developments in Theory and Application

Advanced Materials, EarlyView.
This review comprehensively summarizes the recent progress in the design and fabrication of sensory‐adaptation‐inspired devices and highlights their valuable applications in electronic skin, wearable electronics, and machine vision. The existing challenges and future directions are addressed in aspects such as device performance optimization ...
Guodong Gong, You Zhou, Qingxiu Li, Wenyu Zhao, Sunyingyue Geng, Hangfei Li, Yan‐Bing Leng, Shirui Zhu, Guanglong Ding, Yongbiao Zhai, Ziyu Lv, Ye Zhou, Su‐Ting Han   +12 more
wiley   +1 more source

In Vivo Monitoring of Thrombo‐Inflammatory Biomarkers via Molecularly Imprinted Polymer‐Integrated Hydrogel Microneedles

Advanced Materials, EarlyView.
A wearable electrochemical microneedle patch integrates Prussian Blue redox transduction with molecularly imprinted polymer recognition for reagent‐free sampling and detection of thrombo‐inflammatory biomarkers in dermal interstitial fluid. The platform tracks thrombin and inflammatory cytokines with sensitive in vitro, ex vivo, and in vivo performance,
Mahmoud Ayman Saleh, Peyman GhavamiNejad, Arash Khorrami Jahromi, Sanjana Srikant, Sripadh Guptha Yedire, Fatemeh Keyvani, Roozbeh Siavash Moakhar, Sajad Shiekh, Mackenzie Graham, Joe Quadrilatero, Mahla Poudineh, Sara Mahshid   +11 more
wiley   +1 more source

Reproduction of root-knot nematodes on 16D10 transgenic hairy root lines.

, 2013
(A) Eggs per hairy root. (B) Eggs per gram hairy root. NC is the negative control (pART27 0). 42-L1 and 271-L5 represent the abbreviations of pART27-42 line 1 and pART27-271 line 5, respectively.
Demosthenis Chronis (433792), Gan-Yuan Zhong (433794), Peter Cousins (433793), Yingzhen Yang (87978), Yingyos Jittayasothorn (433791), Xiaohong Wang (93777)   +5 more
core   +1 more source