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Woody Plant Transformation: Current Status, Challenges, and Future Perspectives. [PDF]

Plants (Basel)
Maharjan BK, Islam MT, Muzaffar A, Tschaplinski TJ, Tuskan GA, Chen JG, Yang X.   +6 more
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Genetic transformation using Agrobacterium rhizogenes

Physiologia Plantarum, 1990
Agrobacterium rhizogenes transfers genes encoding phenotypic alterations that serve as morphological markers for the presence of foreign DNA. These alterations facilitate root culture and thus studies of roots and their interactions with their biological and physical environments. They also provide insights into how plants develop.
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Agrobacterium rhizogenes-mediated transformation of Echinacea purpurea

Plant Cell Reports, 1991
Echinacea purpurea seedlings were inoculated with several Agrobacterium rhizogenes strains in order to obtain hairy roots. Infection with A. rhizogenes strains LMG63 and LMG150 resulted in callus formation. Upon infection with strains ATCC 15834 and R1601 hairy roots were obtained. Opine detection confirmed transformation of E. purpurea.
M, Trypsteen, M, Van Lijsebettens, R, Van Severen, M, Van Montagu   +3 more
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Opine biosynthesis and catabolism genes of Agrobacterium tumefaciens and Agrobacterium rhizogenes

Russian Journal of Genetics, 2015
Agrobacterium is a genus of soil bacteria with the ability to transform plant cells by a T-DNA-sequence located on the pTi/pRi- plasmid containing a set of genes expressed in plant cells. Expression of these genes leads to a proliferation of transformed cells, with the subsequent formation of tumors or growths of roots and the synthesis of opines ...
I A, Vladimirov, T V, Matveeva, L A, Lutova   +2 more
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The Oncogenes of Agrobacterium Tumefaciens and Agrobacterium Rhizogenes

2008
The common soil bacteria Agrobacterium tumefaciens and Agrobacterium rhizogenes are unique genetic pathogens capable of fundamentally redirecting plant metabolism in order to generate macroscopic tissue masses (crown galls and hairy roots, respectively) which support the growth of large populations of Agrobacteria.
Monica T. Britton, Matthew A. Escobar, Abhaya M. Dandekar   +2 more
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Tumor formation and rhizogenicity of Agro bacterium rhizogenes carrying Ti plasmids

Gene, 1980
Octopine Ti plasmids were introduced into different Agrobacterium rhizogenes strains. Transconjugants became able to utilize octopine and to induce aberrant tumors, the surface of which was all covered wioth roots of different size and morphology. The plasmids endogenous in A. rhizogenes were shown to be compatible with incRH-1 Ti plasmids.
COSTANTINO, Paolo, Hooykaas, P. J. J., den Dulk Ras, A. , Schilperoort, R.   +4 more
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Transformation of Aster sedifolius via Agrobacterium rhizogenes

2004
In recent years, different strategies of metabolic engineering have been adopted to improve the production of phytochemicals useful for different purposes: pharmaceuticals, cosmetics, food additives, agrochemicals. In this context, the transformation of plants by Agrobacterium rhizogenes is an important achievement.
Cammareri M, Lotti E, Filippone E, Errico A, Conicella C   +4 more
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Regeneration of flax plants transformed by Agrobacterium rhizogenes

Plant Molecular Biology, 1988
Regeneration of flax (Linum usitatissimum) following transformation by either Agrobacterium tumefaciens carrying a disarmed Ti-plasmid vector, or Agrobacterium rhizogenes carrying an unmodified Ri plasmid, was examined. Hypocotyl and cotyledon explants inoculated with A.
X C, Zhan, D A, Jones, A, Kerr
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Agrobacterium rhizogenes-mediated DNA transfer inPinus halepensis Mill.

Plant Cell Reports, 1996
Agrobacterium rhizogenes strain LBA9402 was used to transformPinus halepensis embryos, seedlings and shoots. Mature embryos exhibited susceptibility to the agrobacterium as monitored by β-glucurortidase (GUS) expression, with more than 85% showing considerable transient GUS expression in the radicle.
T, Tzfira, O, Yarnitzky, A, Vainstein, A, Altman   +3 more
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Regions of homology between tumorigenic plasmids from Agrobacterium rhizogenes and Agrobacterium tumefaciens

Plasmid, 1982
Abstract Plasmids from two virulent strains of Agrobacterium rhizogenes belonging to biotypes 1 and 2 are compared for DNA homology with the nopaline Ti plasmid from Agrobacterium tumefaciens C58 by means of Southern blot hybridizations. We find that both A. rhizogenes plasmids share strong sequence homology with regions of the Ti plasmid that affect
RISULEO, Gianfranco, P. Battistoni, COSTANTINO, Paolo   +2 more
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