Results 181 to 190 of about 65,231 (230)
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Transformation of Solanum nigrum L. protoplasts by Agrobacterium rhizogenes
Plant Cell Reports, 1986Solanum nigrum protoplasts were co-cultivated with Agrobacterium rhizogenes harboring agropine-type Ri plasmid (pRi15834). A large number of transformed calli were obtained on Murashige and Shoog's (MS) medium lacking plant growth regulators. Frequency of transformation was about 3.5×10(-3).
Z M, Wei, H, Kamada, H, Harada
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Journal of Visualized Experiments, 2019
Agrobacterium sp. is one of the most widely used methods to obtain transgenic plants as it has the ability to transfer and integrate its own T-DNA into the plant's genome.
S. Fernández-Piñán +5 more
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Agrobacterium sp. is one of the most widely used methods to obtain transgenic plants as it has the ability to transfer and integrate its own T-DNA into the plant's genome.
S. Fernández-Piñán +5 more
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Grapevine root transformation with Agrobacterium rhizogenes
1990VITIS - Journal of Grapevine Research, Vol. 29 (1990): Vitis (Special Issue)
Gribaudo I, SCHUBERT, Andrea
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Plasmid, 1982
Abstract Plasmids from two virulent strains of Agrobacterium rhizogenes belonging to biotypes 1 and 2 are compared for DNA homology with the nopaline Ti plasmid from Agrobacterium tumefaciens C58 by means of Southern blot hybridizations. We find that both A. rhizogenes plasmids share strong sequence homology with regions of the Ti plasmid that affect
RISULEO, Gianfranco +2 more
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Abstract Plasmids from two virulent strains of Agrobacterium rhizogenes belonging to biotypes 1 and 2 are compared for DNA homology with the nopaline Ti plasmid from Agrobacterium tumefaciens C58 by means of Southern blot hybridizations. We find that both A. rhizogenes plasmids share strong sequence homology with regions of the Ti plasmid that affect
RISULEO, Gianfranco +2 more
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Agrobacterium rhizogenes-mediated Transformation and Regeneration of the Apocynum venetum
Chinese Journal of Biotechnology, 2008A system for the Agrobacterium rhizogenes-mediated transformation and plant regeneration of A. venetum has been developed. The highest transformation frequency was 100%, achieved by using strain LBA9402 with root explants. The highest density of hairy roots reached 22 when root explants transformed by R1000 cultured in the dark.
Haiyan, Jia +3 more
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The Agrobacterium Rhizogenes Root-Inducing System
1987In sensitive dicotyledoneous plants, Agrobacterium rhizogenes inoculation in wounds result first in the formation of a small callus that resembles the lesions induced in the same plant hosts by the related pathogen Agrobacterium tumefaciens. The fundamental difference between both pathogens is that in the former, a population of callus cells promptly ...
F. Richaud +7 more
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Infection of turnip and radish storage roots with Agrobacterium rhizogenes
Plant Cell Reports, 1985Within about 10 days after inoculation with Agrobacterium rhizogenes, the vascular bundles of storage root disks of turnip or radish developed small outgrowths with numerous root hairs. Thereafter, adventitious roots (hairy roots) emerged extensively from these outgrowths.
N, Tanaka +4 more
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Characterization of ornamental Datura plants transformed by Agrobacterium rhizogenes
In Vitro Cellular & Developmental Biology - Plant, 1997Datura arborea and D. sanguinea hairy roots were produced by cocultivation of leaf fragments with Agrobacterium rhizogenes strain NCPP 1855. Adventitious buds emerged spontaneously, without exogenous growth regulators, from seven hairy root clones of D. arborea and from one hairy root clone of D. sanguinea.
GIOVANNINI A. +3 more
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Cucurbita pepo L. can be transformed by Agrobacterium rhizogenes
Plant Cell, Tissue and Organ Culture, 1997Two-week-old in vitro grown Cucurbita pepo L. intact plants and cotyledons (detached and undetached from the mother-plant) were transformed by Agrobacterium rhizogenes strain NCPPB 1855, grown for 48 h at 25 °C on YMB medium. All infected material formed vigorous hairy roots in about seven days.
SANITA DI TOPPI L. +2 more
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Organization and characterization of the virCD genes from Agrobacterium rhizogenes
Molecular and General Genetics MGG, 1988We have precisely localized virulent (vir) genes of the hairy root-inducing plasmid pRiA4b on the basis of sequence similarity with the tumor-inducing plasmid pTiA6NC, and shown that the overall organizations of vir genes in both plasmids are fairly analogous, although sizes and spacer lengths in some genes differ from each other.
T, Hirayama +3 more
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