Results 81 to 90 of about 39,002 (241)

Engineering Gypsophila elegans hairy root cultures to produce endosomal escape‐enhancing saponins

Plant Biotechnology Journal, EarlyView.
Summary The limited cytosolic delivery of DNA and protein‐based therapeutics due to endosomal entrapment reduces drug efficacy, increasing treatment costs and possible side effects in human and veterinary medicine as a consequence of higher administered dosages.
Elia Lacchini, Tongtong Qu, Tessa Moses, Alexander N. Volkov, Alain Goossens   +4 more
wiley   +1 more source

LRM3 positively regulates stem lodging resistance by degradating MYB6 transcriptional repressor in soybean

Plant Biotechnology Journal, EarlyView.
Summary Stem lodging resistance plays a critical role in maintaining soybean yield stability, yet the molecular mechanisms governing stem development and lodging tolerance remain poorly understood. Here, we report the characterization of lodging‐related mutant 3 (lrm3), a weak‐stemmed soybean line exhibiting increased lodging susceptibility.
Yongheng Ye, Zhiyuan Cheng, Xinjing Yang, Suxin Yang, Kuanqiang Tang, Hui Yu, Jinshan Gao, Yaohua Zhang, Jiantian Leng, Wei Zhang, Ye Zhang, Moran Bu, Zhengwei Liang, Zhicheng Dong, Zhonghui Zhang, Xianzhong Feng   +15 more
wiley   +1 more source

Improved Agrobacterium rhizogenes-mediated hairy root culture system of Withania somnifera (L.) Dunal using sonication and heat treatment

3 Biotech, 2015
An improved Agrobacterium rhizogenes-mediated genetic transformation of Withania somnifera (L.) Dunal was developed using the bacterial strain R1000 with leaf segment explants of in vitro raised plantlets.
Chandrasekaran Thilip, C. Soundar Raju, K. Varutharaju, A. Aslam, A. Shajahan   +4 more
semanticscholar   +1 more source

Dt1 interacts with HB40 to affect lateral root primordium development by regulating CDC48 in soybean

Plant Biotechnology Journal, EarlyView.
Summary Roots act as the plants' primary determinants of the uptake of water and nutrients, while the root structure largely depends on the repeated formation of new lateral roots (LR). Lateral root primordium (LRP) formation defines the organization and function of LRs.
Haiyang Li, Sijia Fang, Lanxin Li, Jingwen Peng, Ziyuan Deng, Chenyang Lin, Chuanjie Gou, Ke Zhang, Baohui Liu, Fanjiang Kong, Weijun Ye, Huan Liu   +11 more
wiley   +1 more source

New Integrative Vectors Increase Agrobacterium rhizogenes Transformation and Help Characterise Roles for Soybean GmTML Gene Family Members

Plant, Cell &Environment, EarlyView.
ABSTRACT Hairy‐root transformation is widely used to generate transgenic plant roots for genetic functional characterisation studies. However, transformation efficiency can be limited, largely due to the use of binary vectors. Here, we report on the development of novel integrative vectors that significantly increase the transformation efficiency of ...
Huanan Su, Mengbai Zhang, Estelle B. Grundy, Brett J. Ferguson   +3 more
wiley   +1 more source

Recent Advances in Cotton Transformation and Genome Editing Techniques: The Prospects and Challenges

Modern Agriculture, Volume 3, Issue 1, June 2025.
Application of transformed cotton plants. Cotton transformation yields desirable traits, including: (1) strengthening insect and pest resistance, (2) enhancing the resistance to abiotic stress such as drought, heat, cold and salinity, (3) improving herbicide tolerance, (4) increasing the cotton lint and seed yield, (5) boosting nutrient uptake ...
Oluwaseun Olayemi Aluko, Vincent Ninkuu, Zhixin Liu, Aizhi Qin, Hao Liu, Jincheng Yang, Mengke Hu, Xiongfeng Ma, Xuwu Sun   +8 more
wiley   +1 more source

IMPROVEMNET OF HAIRY ROOT INDUCTION IN Artemisia annua BY VARIOUS STRAINS OF Agrobacterium rhizogenes

, 2017
Plants have been considered as a significant source of drugs since ancient times. Secondary metabolites derived from plants have revealed therapeutic properties.
S. Hassan, Zahra Belb Belbasi
semanticscholar   +1 more source

Establishment of Hairy Root Cultures by Agrobacterium Rhizogenes Mediated Transformation of Isatis Tinctoria L. for the Efficient Production of Flavonoids and Evaluation of Antioxidant Activities

PLoS ONE, 2015
In this work, Isatis tinctoria hairy root cultures (ITHRCs) were established as an alternative source for flavonoids (FL) production. I. tinctoria hairy root line V was found to be the most efficient line and was further confirmed by the PCR ...
Qing-Yan Gai, Jiao Jiao, Meng Luo, Zuo-fu Wei, Y. Zu, Wei Ma, Yu-jie Fu   +6 more
semanticscholar   +1 more source

Effect of pectinase on the production of sesquiterpene lactones in the hairy root culture of Lactuca virosa L.

Acta Societatis Botanicorum Poloniae, 2014
Direct addition of pectinase as an elicitor to the hairy root culture of Lactuca virosa L. transformed with Agrobacterium rhizogenes stimulates the accumulation of constitutive sesquiterpene lactones - lactuside A and crepidiaside B.
Janusz Malarz, Wanda Kisiel
doaj   +1 more source

Overexpression of the GmERF071 gene confers resistance to soybean cyst nematode in soybean

The Plant Genome, Volume 18, Issue 2, June 2025.
Abstract Soybean cyst nematode (SCN) is one of the most harmful pests, causing major reductions in soybean yield globally. The validation and functional characterization of SCN resistance genes are crucial to improving soybean yield worldwide. Herein, we describe an SCN resistance gene, GmERF071 (Glyma.19g262700).
Erhui Xiong, Jiaqi Xu, Pingzhang Feng, Yun Lian, Xiaoling Zhi, Ke Li, Erhan Zhang, Bing Li, Shijie Zhao, Changzhong Liu, Chengyu Wei, Panpan Li, Yaping Zhao, Lipei Zhao, Mengwei Zheng, Heng Zhang, Yi Li, Shanshan Chu, Yongqing Jiao   +18 more
wiley   +1 more source