Results 81 to 90 of about 9,389 (220)
The procedure for vacuum infiltration of cultivars A. caudatus L. and hybrids of A. caudatus L. xA. paniculatus L. was optimized. The functioning of gene construction pCBv19 in the Amaranthus leaves was evaluated by the transient expression after vacuum ...
Olha Mikolaivna Yaroshko +1 more
doaj +1 more source
AGROBACTERIUM-MEDIATED TRANSFORMATION OF COMPOSITAE PLANTS. I. CONSTRUCTION OF TRANSGENIC PLANTS AND «HAIRY» ROOTS WITH NEW PROPERTIES [PDF]
The review explores some of the recent advances and the author's own researchs concerning biotechnological approaches for Agrobacterium tumefaciens- and A. rhizogenes-mediated transformation of Compositae family plants.
N. A.Matvieieva
doaj +1 more source
ABSTRACT Hairy‐root transformation is widely used to generate transgenic plant roots for genetic functional characterisation studies. However, transformation efficiency can be limited, largely due to the use of binary vectors. Here, we report on the development of novel integrative vectors that significantly increase the transformation efficiency of ...
Huanan Su +3 more
wiley +1 more source
En el mejoramiento de los cultivos ornamentales la Ingeniería Genética y la transformación genética de plantas son herramientas de gran utilidad para incrementar, artificialmente, la variabilidad genética.
María Cristina Gennarelli +2 more
doaj +1 more source
Establishing monoxenic culture of arbuscular mycorrhizal fungus Glomus intraradices through root organ culture [PDF]
Arbuscular mycorrhizal fungi are soil fungi distributed worldwide, forming symbiosis with most of the vascular plants for their growth and survival, which is used for sustainable agriculture and ecosystem management.
Kumar, K. +3 more
core +2 more sources
ABSTRACT Evidence suggests that the metabolism of inositol and its derivatives may be involved in various biological processes including salt tolerance, but there has been limited understanding. Ectopic expression of Gs5PTase8, an inositol polyphosphate 5‐phosphatase cloned from wild soybean (Glycine soja), significantly enhanced salt tolerance in ...
Qi Jia +12 more
wiley +1 more source
The promoter from SlREO, a highly-expressed, root-specific Solanum lycopersicum gene, directs expression to cortex of mature roots [PDF]
Root-specific promoters are valuable tools for targeting transgene expression, but many of those already described have limitations to their general applicability.
Abe +68 more
core +1 more source
ABSTRACT Isoflavone synthase (IFS), a cytochrome P450 monooxygenase of the CYP93C subfamily, catalyzes the conversion of flavanones into isoflavones, the first committed step in the biosynthesis of isoflavonoid phytoalexins. In pea (Pisum sativum L.), the phytoalexin pisatin plays a pivotal role in defense against pathogens.
Muhammad Sufyan Tahir +4 more
wiley +1 more source
Agrobacterium rhizogenes Transformation of the Phaseolus spp.: A Tool for Functional Genomics
A fast, reproducible, and efficient transformation procedure employing Agrobacterium rhizogenes was developed for Phaseolus vulgaris L. wild accessions, landraces, and cultivars and for three other species belonging to the genus Phaseolus: P.
Georgina Estrada-Navarrete +12 more
doaj +1 more source
A Universal Hairy Root Transformation Protocol for Evaluating Editing Efficiency in Dicot Plants
A streamlined workflow for the rapid evaluation of CRISPR‐Cas9 editing efficiency using a universal Agrobacterium rhizogenes–mediated hairy root transformation system. The process encompasses seed germination, explant preparation, bacterial infection, hairy root induction, and comprehensive validation of editing events via Sanger sequencing, PCR ...
Hong Yang +5 more
wiley +1 more source

