Results 111 to 120 of about 80,673 (331)

Transformación eficiente de Solanum tuberosum cv. Spunta mediada por Agrobacterium tumefaciens utilizando higromicina como agente selectivo

open access: yesRIA: Revista Investigaciones Agropecuarias, 2020
La transformación genética de plantas es una herramienta biotecnológica de alto impacto en el mejoramiento vegetal que brinda la posibilidad de obtener nuevos cultivos que se adapten a las demandas de producción y de consumo. Actualmente la estrategia de
C. DÉCIMA ONETO   +5 more
doaj  

Cellulose Synthesis in Agrobacterium tumefaciens [PDF]

open access: yes, 2004
We have cloned the celC gene and its homologue from E. coli, yhjM, in an expression vector and expressed the both genes in E. coli; we have determined that the YhjM protein is able to complement in vitro cellulose synthesis by extracts of A. tumefaciens celC mutants, we have purified the YhjM protein product and are currently examining its enzymatic ...
White, Alan R., Matthysse, Ann G.
openaire   +3 more sources

Control of two sucking insect pests, a whitefly (Bemisia tabaci) and a thrips (Frankliniella occidentalis), infesting hot peppers by spraying LDH‐formulated dsRNA

open access: yesPest Management Science, EarlyView.
A vacuolar type ATPase subunit B (vATPase‐B) gene is identified and expressed in the midgut of Bemisia tabaci and Frankliniella occidentalis. The double‐stranded RNA (dsRNA) specific to vATPase‐B was expressed in hot peppers by virus‐induced gene silencing (VIGS) and killed both whitefly and thrips.
Falguni Khan   +2 more
wiley   +1 more source

Evaluation of fluorescence-activated cell sorting technology in agrobacterium biocontrol

open access: yesJournal of Crop Protection, 2021
Fluorescence-activated cell sorting (FACS) as a novel and sensitive technology was used to evaluate the biocontrol efficiency of Bacillus subtilis against Agrobacterium tumefaciens, a very destructive plant pathogen.
Fahimeh Nazari   +2 more
doaj  

A Method for High‐Throughput Screening of Monoclonal Antibody Internalization Using a DNA/Protein Molecular Staple

open access: yesSmall Methods, EarlyView.
Internalization of antibodies plays a critical role in targeted drug delivery and the activity of antibody therapeutics. A simple, one‐pot, chemical modification free approach is developed to quantify antibody internalization into cells. Abstract The use of monoclonal antibodies (mAbs) as therapeutics or for targeted delivery to specific cells is ...
Lara M. Mollé   +4 more
wiley   +1 more source

Inhibition of Agrobacterium-Induced Cell Death by Antiapoptotic Gene Expression Leads to Very High Transformation Efficiency of Banana

open access: yesMolecular Plant-Microbe Interactions, 2007
The death of plant cells in culture following exposure to Agrobacterium tumefaciens remains a major obstacle in developing Agrobacterium-mediated transformation into a highly efficient genotype-independent technology.
Harjeet K. Khanna   +4 more
doaj   +1 more source

Ecological graph theory: Simulating competition and coexistence on graphs

open access: yesMethods in Ecology and Evolution, EarlyView.
Abstract Since its inception, the evolutionary graph theory (EGT) has been extensively used to study the fixation probability, the rate of evolution, game‐theoretical topics and related questions in a spatially structured framework. However, few attempts have been made to use this framework in ecology.
Ata Kalirad, Ralf J. Sommer
wiley   +1 more source

Utilisation d'Agrobacterium tumefaciens et Agrobacterium rhizogenes comme vecteurs de clonage d'un gène dans la tomate et le tournesol

open access: yesRevue Marocaine des Sciences Agronomiques et Vétérinaires, 1995
L'introduction d'un gène Pl provenant d'un. champignon Cladosporum fulvum,dans le génome de deux plantes supérieures, la tomate et le tournesol, a été réalisée en utilisant Agrobacterium tumefaciens et Agrobacterium rhizogenes comme vecteurs. Le gène Pl
C. Alem   +3 more
doaj  

Agrobacterium Mediated Transformation of Maize (Zea mays L.) [PDF]

open access: yesJournal of Sciences, Islamic Republic of Iran, 2010
Agrobacterium tumefaciens mediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize plants. This gene delivery system results in a greater proportion of stable, low-copy number transgenic events ...
H. Rahnama
doaj  

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