Results 121 to 130 of about 77,503 (330)

Identification and characterization of a cucumber accession resistant to tomato leaf curl New Delhi virus

open access: yesAnnals of Applied Biology, EarlyView.
Tomato leaf curl New Delhi virus (ToLCNDV) causes significant yield and fruit quality losses in cucurbit crops, particularly in the Mediterranean region. We identified a new source of resistance to ToLCNDV in cucumber (accession ABS.PE.045) and mapped resistance to four genomic intervals in chromosomes 1, 2, 6, and 7.
Livia Donaire   +5 more
wiley   +1 more source

Detection of mutations induced by CRISPR/Cas9 in cabbage protoplasts using qPCR and dPCR

open access: yesAnnals of Applied Biology, EarlyView.
This study evaluated two PCR‐based methods—qPCR and dPCR—for detecting CRISPR‐induced mutations in transformed cabbage protoplasts. Both methods distinguished edited from wild‐type protoplasts, offering rapid and cost‐efficient alternatives for mutation detection, with dPCR providing higher sensitivity and closer agreement with NGS results.
Ester Stajič   +2 more
wiley   +1 more source

Factors affecting genetic transformation via Agrobacterium tumefaciens in Phaseolus vulgaris L.

open access: yesBiotecnología Vegetal, 2013
Phaseolus genus, as well as other legumes, has proved recalcitrant plant regeneration in vitro and mainly to genetic transformation via Agrobacterium tumefaciens.
Amanda Martirena-Ramírez   +1 more
doaj  

Solanum bulbocastanum nucleotide‐binding leucine‐rich repeat receptor evolution reveals functional variants and critical residues in Rpi‐blb1/RB

open access: yesJournal of Integrative Plant Biology, EarlyView.
Natural variants of the nucleotide‐binding leucine‐rich repeat protein Rpi‐blb1/RB in Solanum bulbocastanum recognize the Phytophthora infestans effector IPI‐O4, as well as two non‐functional variants. The S347N mutation disrupts IPI‐O1 recognition, Rpi‐blb1/RB self‐association and subcellular relocalisation, thereby compromising resistance.
Jie Li   +18 more
wiley   +1 more source

TaIRE1‐mediated unconventional splicing of the TabZIP60 mRNA and the miR172 precursor regulates heat stress tolerance in wheat

open access: yesJournal of Integrative Plant Biology, EarlyView.
In wheat, INOSITOL‐REQUIRING ENZYME 1 (IRE1) helps plants cope with heat stress by regulating unconventional splicing of the mRNA encoding the bZIP transcription factor TabZIP60, which activates heat‐responsive genes, and cleavage of the precursor of the microRNA miR172 during heat stress.
Haoran Li   +15 more
wiley   +1 more source

Development of cytosine and adenine base editors for maize precision breeding

open access: yesJournal of Integrative Plant Biology, EarlyView.
A series of maize cytosine and adenine base editors was developed using various deaminase variants, enabling efficient multiplex genome editing in maize. Precise editing of the ZmACC1/2 genes generated herbicide‐tolerant maize germplasm, offering a solution for weed management in maize‐soybean intercropping systems.
Xiao Fu   +8 more
wiley   +1 more source

U.S. Patent Literature Survey of Agrobacterium-Mediated Transformation of Sweet Potato (Ipomoea Batatas) [PDF]

open access: yes, 2007
A team of researchers and patent information scientists at Franklin Pierce Law Center were asked to evaluate the patent and literature landscape related to the Agrobacterium-mediated transformation in sweet potato with respect to the U.S.
Cavicchi, Jon R.   +3 more
core   +1 more source

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