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Agrobacterium tumefaciens-Mediated Transformation of Tomato
2018Tomato is both an important food crop and serves as a model plant species that is used for various research investigations including understanding gene function. Transformation is commonly utilized to facilitate these investigations in combination with all the extensive genetic and genomic resources available for tomato.
Joyce, Van Eck +2 more
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Agrobacterium tumefaciens-Mediated Transformation
2014The use of Agrobacterium tumefaciens-mediated transformation for achieving genetic transformation of fungi has steadily increased over the last decade, and has proven to be almost universally applicable technique once suitable selection markers have been developed.
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2014
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???????????????? ???????????????????????? ???????????????????? ?????????????????????? ???????????????????? ???? ?????????????????? ?? ????????????????, ???????????????????????????? A. tumefaciens ?? ???????????????? ?????????????????? ?????????????? ??????????????????. ?? ???????????? ???????????????????? ???????????? ?????????????????? ????????????, ??
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Agrobacterium tumefaciens-mediated transformation ofMucor circinelloides
Folia Microbiologica, 2005The Agrobacterium tumefaciens-mediated transformation of the zygomycetous fungus Mucor circinelloides is described. A method was also developed for the hygromycin B-based selection of Mucor transformants. Transformation with the hygromycin B phosphotransferase gene of Escherichia coli controlled by the heterologous Aspergillus nidulans trpC promoter ...
I, Nyilasi +4 more
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2019
?????????????? ?????????????? ????-?????????????????? ???????????????????????? ???????????? A. tumefaciens ???? ?????????????? ???????????????? ???????????????????? ?????????? ?? ????-????????????????, ?????????????????????????????? ?? ?????????????? ?????????????????????????? ????????????????. ????????????????, ?????? ?????????????????????????? ???????
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?????????????? ?????????????? ????-?????????????????? ???????????????????????? ???????????? A. tumefaciens ???? ?????????????? ???????????????? ???????????????????? ?????????? ?? ????-????????????????, ?????????????????????????????? ?? ?????????????? ?????????????????????????? ????????????????. ????????????????, ?????? ?????????????????????????? ???????
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Exploration of Agrobacterium tumefaciens
1992Bacteria of the genus Agrobacterium are soil-borne plant pathogens. Their unique contribution to biology consists of their natural system for delivering DNA into host plants, where it becomes integrated and thereby stabilized in the genome. As a consequence of expression of bacterial DNA in the plants, the normal developmental pattern is changed ...
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Preparation of Chemocompetent Agrobacterium tumefaciens Cells
Agrobacterium tumefaciens, a soilborne bacterium known for its natural ability to transfer genetic material into plant cells, revolutionized molecular biology and plant genetic engineering. To harness this transformative potential, researchers require competent Agrobacterium cells capable of efficiently delivering desired genetic constructs into plant ...Samuel, Parra, Claudia, Stange Klein
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Transposon-Induced Catalase-Deficient Agrobacterium tumefaciens
Current Microbiology, 1997Agrobacterium tumefaciens MKR, a nonpathogenic strain, has three catalase isozymes and one superoxide dismutase but no detectable peroxidase activity. A large number (8400) of transconjugants were obtained with pSUP1011::Tn5 suicide vector. The transposition frequencies were found to be greater in biparental mating than in triparental mating with ...
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The Oncogenes of Agrobacterium Tumefaciens and Agrobacterium Rhizogenes
2008The common soil bacteria Agrobacterium tumefaciens and Agrobacterium rhizogenes are unique genetic pathogens capable of fundamentally redirecting plant metabolism in order to generate macroscopic tissue masses (crown galls and hairy roots, respectively) which support the growth of large populations of Agrobacteria.
Monica T. Britton +2 more
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Agrobacterium tumefaciens and Interkingdom Genetic Exchange
Annual Review of Phytopathology, 1989This review considers four fundamental questions that comprise the heart of the agrobacterium story. How does interkingdom genetic exchange occur? How do the host and pathogen communicate, and what do they say to each other? How do enzymes encoded by the T-DNAs of A. tumefaciens and A. rhizogenes transform host cells into tumor cells?
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