Results 171 to 180 of about 80,673 (331)

Highly efficient transgene‐free ErCas12a RNP‐protoplast genome editing and single‐cell regeneration in Nicotiana benthamiana for glyco‐engineering

open access: yesPlant Biotechnology Journal, EarlyView.
Summary Nicotiana benthamiana serves as a unique platform for biopharmaceutical production, offering advantages such as efficient and scalable protein synthesis. In addition, custom N‐glycans can be engineered on biopharmaceutical glycoproteins. Yet, plant‐native glycosyltransferases and glycoside hydrolases need to be removed to prevent undesired ...
Lukas C. Blumberg   +8 more
wiley   +1 more source

Expression of a constitutively active nitrate reductase increases SARS‐CoV‐2 Spike protein production in Nicotiana benthamiana leaves that otherwise show traits of senescence

open access: yesPlant Biotechnology Journal, EarlyView.
Summary The production of coronavirus disease 2019 vaccines can be achieved by transient expression of the Spike (S) protein of Severe Acute Respiratory Syndrome Coronavirus 2 in agroinfiltrated leaves of Nicotiana benthamiana, a process promoted by the co‐expression of viral silencing suppressor P19.
Louis‐Philippe Hamel   +9 more
wiley   +1 more source

Agrobacterium tumefaciens-Mediated Transformation of the Aquatic Fungus Phialemonium inflatum FBCC-F1546. [PDF]

open access: yesJ Fungi (Basel), 2023
Yoon J   +12 more
europepmc   +1 more source

Complex engineering of Solanum alkaloids structural diversity in Nicotiana benthamiana

open access: yesPlant Biotechnology Journal, EarlyView.
Summary Transient expression in Nicotiana benthamiana offers a powerful and versatile platform for rapid production of complex specialized metabolites. Steroidal glycoalkaloids (SGAs) and steroidal saponins produced by members of the Solanaceae family are known for their diverse structures and activities including antimicrobial, anticancer, antiviral ...
Sachin A. Gharat   +6 more
wiley   +1 more source

A plant cell‐based platform for the expression of complex proteins with fucose‐reduced sialylated N‐glycans

open access: yes
Plant Biotechnology Journal, EarlyView.
Saeideh Dianatkhah   +10 more
wiley   +1 more source

Developing downstream processes for the purification of recombinant proteins and small molecules from Nicotiana benthamiana biomass

open access: yesPlant Biotechnology Journal, EarlyView.
Summary Nicotiana benthamiana (Australian tobacco) has become a major host for plant‐based recombinant protein production, especially using transient expression. Once a candidate protein has been designed and produced in a suitable variety (e.g. facilitated humanized glycosylation) under reproducible conditions (e.g.
J. F. Buyel
wiley   +1 more source

A Study on the Use of the Phyto-Courier Technology in Tobacco Leaves Infected by Agrobacterium tumefaciens. [PDF]

open access: yesInt J Mol Sci, 2023
Gutsch A   +7 more
europepmc   +1 more source

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