Single‐Cell CRISPR: An Efficient Strategy for Decoding Plant Cis‐Regulatory Complexity
ABSTRACT The generation of complex traits involves the coordinated interplay of multiple gene networks. Elucidating the function of transcriptional cis‐regulatory elements (CREs) in regulating gene expression is crucial for understanding complex regulatory pathways and improving our ability to modify macro‐phenotypes.
Yuanhang Zhang +5 more
wiley +1 more source
Simple and Fail-safe Method to Transform Miniprep Escherichia coli Strain K12 Plasmid DNA Into Viable Agrobacterium tumefaciens EHA105 Cells for Plant Genetic Transformation. [PDF]
Siamalube B +4 more
europepmc +1 more source
ABSTRACT To safeguard global food security against rapid population growth and a warming world, the effective genetic improvement of cereals is imperative. Flower opening time (FOT) critically affects the seed setting rate. In this study, we identified a gene, EARLY‐MORNING FLOWERING 3 (EMF3), in which single‐nucleotide substitutions strongly modulate ...
Takuma Ishizaki +18 more
wiley +1 more source
A peptidoglycan N-deacetylase specific for anhydroMurNAc chain termini in Agrobacterium tumefaciens. [PDF]
Gilmore MC +6 more
europepmc +1 more source
ABSTRACT Plant glandular trichomes are specialised epidermal structures capable of synthesising, storing and secreting numerous varieties of secondary metabolites in different classes and are central to plant defence and the biosynthesis of high‐value metabolites.
Hai Liu +4 more
wiley +1 more source
Control of biofilm formation by an Agrobacterium tumefaciens pterin-binding periplasmic protein conserved among diverse Proteobacteria. [PDF]
Greenwich JL +11 more
europepmc +1 more source
CitPH4 Confers Resistance to Citrus Canker by Activating Papain‐Like Cysteine Protease
ABSTRACT Citrus canker, a devastating disease caused by Xanthomonas citri subsp. citri (Xcc), poses a significant threat to global citrus production due to the high susceptibility of nearly all commercial citrus cultivars to it. Although transcription factor Citrus PH4 (CitPH4) is well known for regulating fruit acidity, its potential role in plant ...
Tao Yuan +20 more
wiley +1 more source
Structure-function analysis of the cyclic β-1,2-glucan synthase from Agrobacterium tumefaciens. [PDF]
Sedzicki J +4 more
europepmc +1 more source
Electroporation of Agrobacterium tumefaciens
Dulk, H. den, Hooykaas, P.J.J.
openaire +1 more source
ABSTRACT Anthracnose caused by Colletotrichum gloeosporioides is a major threat to tea cultivation; however, the molecular mechanism underlying different resistance among tea cultivars remains unclear. We identified distinct expression patterns of CsMYB82 between anthracnose‐resistant and susceptible varieties after infection with anthracnose from ...
Rui Han +14 more
wiley +1 more source

