Results 231 to 240 of about 67,194 (299)

A high-efficiency transient expression system mediated by Agrobacterium tumefaciens in Spinacia oleracea leaves. [PDF]

Plant Methods
Zhang Y, Qiu L, Zhang Y, Wang Y, Fu C, Dai S, Sun M.   +6 more
europepmc   +1 more source

Extracts From Perilla frutescens and Artemisia argyi Offer a Novel Strategy for Sustainable Integrated Management of Citrus Huanglongbing

Journal of Sustainable Agriculture and Environment, Volume 5, Issue 3, September 2026.
Schematic summarizing the triple functions of Perilla frutescent and Artemisia argyi extract against the citrus HLB, encompassing psyllid repellency, plant immune activation and pathogen reduction. ABSTRACT Citrus Huanglongbing (HLB), a devastating global citrus disease caused by the unculturable phloem‐limited bacterium Candidatus Liberibacter ...
Libo Wu, Weiping Zhu, Shaolong Zhang, Hang Chen, Yu Shi, Chunxi Zhao, Gecheng Ouyang, Hao Liu, Xiaolin Xie, Wenli Chen   +9 more
wiley   +1 more source

An Effective Somatic-Cell Regeneration and Genetic Transformation Method Mediated by Agrobacterium tumefaciens for Portulaca oleracea L. [PDF]

Plants (Basel)
Xu M, Zhao X, Fang J, Yang Q, Li P, Yan J.   +5 more
europepmc   +1 more source

Electroporation of Agrobacterium tumefaciens

, 1995
Dulk, H. den, Hooykaas, P.J.J.
openaire   +1 more source

Natural variation in the atypical resistance gene Ptr confers broad‐spectrum neck blast resistance in rice

The Plant Genome, Volume 19, Issue 3, September 2026.
Abstract Neck blast (NB), caused by Magnaporthe oryzae, infects rice (Oryza sativa L.) and reduces yield. Knowledge of NB resistance remains limited due to the lack of reliable resistance evaluation methods. Here, we applied a newly established neck injection method and performed a genome‐wide association study (GWAS) on 335 diverse accessions from the
Ian Paul Navea, Mohammad Abdul Monsur, Mary Jeanie Telebanco‐Yanoria, Dianne Gene De La Rosa, Sherry Lou Hechanova, Arvin Paul Tuaño, Christian Joseph Cumagun, Il‐Ryong Choi, Suresh Kadaru, Sung‐Ryul Kim, Bo Zhou, Van Schepler‐Luu   +11 more
wiley   +1 more source

A peptidoglycan N-deacetylase specific for anhydroMurNAc chain termini in Agrobacterium tumefaciens. [PDF]

J Biol Chem
Gilmore MC, Yadav AK, Espaillat A, Gust AA, Williams MA, Brown PJB, Cava F.   +6 more
europepmc   +1 more source

Coupling of both a transactivation module and a double‐stranded DNA‐binding domain boosts Cas12i3 variant‐based cytosine and adenine editing in plants

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3196-3207, September 2026.
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia   +7 more
wiley   +1 more source

Simple and Fail-safe Method to Transform Miniprep Escherichia coli Strain K12 Plasmid DNA Into Viable Agrobacterium tumefaciens EHA105 Cells for Plant Genetic Transformation. [PDF]

Bio Protoc
Siamalube B, Ehinmitan E, Ngotho M, Onguso J, Runo S.   +4 more
europepmc   +1 more source

Enhanced exonuclease–Cas9 systems promote multiple nucleotide deletions with higher efficiency and broader targeting scope in plants

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3316-3328, September 2026.
Cas9 preferentially generates small indels. By tethering an exonuclease and a DNA‐binding domain (DBD) to Cas9, the Exo‐DBD‐Cas9 system efficiently promotes multiple nucleotide deletions. These expanded deletions enable effective disruption of miRNA‐mediated regulation and relieve translational repression through targeted editing of 3′ untranslated ...
Rui Zhang, Xu Tang, Yao He, Wei Wang, Qiurong Ren, Yiping Qi, Yong Zhang   +6 more
wiley   +1 more source

Synergistic engineering of Casδ nuclease for robust genome editing

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3231-3242, September 2026.
A hierarchical engineering strategy was used to enhance the editing activity of Casδ, yielding an optimized variant termed enCasδ. This variant enables robust genome editing in animal cells and plants, with overall editing performance comparable to that of SpCas9 and other Cas12 nucleases.
Fanghui Ge, Chenchen Peng, Yang Du, Ying Chen, Zilong Zhao, Meixia Yu, Huairu Feng, Yuyang Xie, Siwei Sun, Shengnan Liu, Beibei Xin, Haiming Zhao, Sen Wu, Chao Bian, Zhijia Yang, Jinsheng Lai, Jian Chen   +16 more
wiley   +1 more source