Results 251 to 260 of about 77,163 (298)

Transfection and transformation of Agrobacterium tumefaciens

Molecular and General Genetics MGG, 1978
The freeze thaw transfection procedure of Dityatkin et al. (1972) was adapted for the transfection and transformation of A. tumefaciens. Transfection of the strains B6S3 and B6-6 with DNA of the temperate phage PS8cc186 yielded a maximum frequency of 2 10(-7) transfectants per total recipient population.
M, Holsters, D, de Waele, A, Depicker, E, Messens, M, van Montagu, J, Schell   +5 more
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Agrobacterium tumefaciens Peritonitis Mimicking Tuberculosis

Clinical Infectious Diseases, 1992
Agrobacterium species have been previously implicated in the development of clinical disease. We report what we believe to be the first case of ascites caused by Agrobacterium tumefaciens in a cirrhotic patient. Since the correct diagnosis was made only after laparoscopy-guided collection of specimens from two different tissues, we suggest that ...
F C, Ramirez, Z A, Saeed, R O, Darouiche, R M, Shawar, B, Yoffe   +4 more
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Agrobacterium tumefaciens-Mediated Wheat Transformation

Cereal Research Communications, 2003
An efficient and reproducible Agrobacterium tumefaciens-mediated system for wheat transformation was developed and genetically transformed wheat plants were produced using precultured immature embryos as the expiant. The embryos were inoculated with a disarmed A.
Haliloglu, K, Baenziger, PS
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Particulate Cytochrome c in Agrobacterium tumefaciens

Archives Internationales de Physiologie et de Biochimie, 1975
In Agrobacterium tumefaciens the main part of c-type cytochromes is tightly bound to the bacterial cell envelope structures. Several techniques were attempted to solubilize these cytochromes. The highest yield of cytochromes released is obtained by treatment of particle suspensions with 5% Triton X-100.
Van Den Branden, Christiane, Van Beeumen, J., De Ley, Jozef   +2 more
openaire   +3 more sources

Specificity patterns of Agrobacterium tumefaciens phages

Archiv f�r Mikrobiologie, 1970
Lysogeny was not detected in 10 strains of A. tumefaciens by plating techniques or ultra-violet induction. Fifteen phages were isolated from raw sewage against 13 cultures of A. tumefaciens and purified by single-plaque selections. No phage lysed all of the strains of A.
R J, Boyd, A C, Hildebrandt, O N, Allen
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l-Sorbose metabolism in Agrobacterium tumefaciens

Antonie van Leeuwenhoek, 1976
The pathway of L-sorbose metabolism in Agrobacterium tumefaciens strain B6 was determined to be: L-sorbose leads to D-glucitol (sorbitol) leads to D-fructose leads to D-fructose-6-phosphate leads to D-glucose-6-phosphate. The reduction of L-sorbose and the oxidation of D-glucitol were mediated by NADPH- and NAD+-linked oxidoreductases, respectively ...
C, Van Keer, K, Kersters, J, De Ley
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Exopolysaccharides of Agrobacterium tumefaciens

2018
Agrobacterium exopolysaccharides play a major role in the life of the cell. Exopolysaccharides are required for bacterial growth as a biofilm and they protect the bacteria against environmental stresses. Five of the exopolysaccharides made by A. tumefaciens have been characterized extensively with respect to their structure, synthesis, regulation, and ...
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Small Noncoding RNAs in Agrobacterium tumefaciens

2018
During the last decade, small noncoding RNAs (ncRNAs) have emerged as essential post-transcriptional regulators in bacteria. Nearly all important physiological and stress responses are modulated by ncRNA regulators, such as riboswitches, trans-acting small RNAs (sRNAs), and cis-antisense RNAs.
Keunsub, Lee, Kan, Wang
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Exploitation of Agrobacterium tumefaciens

1992
During invasion of wounded plants, soil agrobacteria transfer a defined segment of their Ti and Ri plasmids into the plants. The transferred DNA, termed T-DNA, is integrated into the plant nuclear genome. Genes encoded by Ti and Ri plasmid T-DNAs are expressed in plants and confer the synthesis of plant growth factors as well as sugar and amino acid ...
Csaba Koncz, Jozef Schell
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A chemotaxis cluster from Agrobacterium tumefaciens

Gene, 1998
We report the DNA sequence of a 9.6-kb region of the Agrobacterium tumefaciens chromosome containing a putative 8-kb chemotaxis operon. The putative operon begins with orf1, whose predicted protein product shows strong sequence identity to methyl-accepting chemotaxis proteins (MCPs), followed by orf2, cheY1, cheA, cheR, cheB, cheY2, orf9, orf10. All of
E L, Wright, W J, Deakin, C H, Shaw
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