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Exopolysaccharides of Agrobacterium tumefaciens
2018Agrobacterium exopolysaccharides play a major role in the life of the cell. Exopolysaccharides are required for bacterial growth as a biofilm and they protect the bacteria against environmental stresses. Five of the exopolysaccharides made by A. tumefaciens have been characterized extensively with respect to their structure, synthesis, regulation, and ...
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Transformation of Poplar by Agrobacterium tumefaciens
Nature Biotechnology, 1986The ability to regenerate plants from poplar cells cultured in vitro suggests that poplars may prove a valuable model system for the application of recombinant DNA technology to deciduous trees. We report here the transformation of a hybrid Populus trichocarpa × deltoides with two strains of Agrobacterium tumefaciens, as demonstrated by phytohormone ...
R. F. Stettler+4 more
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Transformation of rice mediated by Agrobacterium tumefaciens
1997Agrobacterium tumefaciens has been routinely utilized in gene transfer to dicotyledonous plants, but monocotyledonous plants including important cereals were thought to be recalcitrant to this technology as they were outside the host range of crown gall. Various challenges to infect monocotyledons including rice with Agrobacterium had been made in many
Yukoh Hiei+2 more
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Small Noncoding RNAs in Agrobacterium tumefaciens
2018During the last decade, small noncoding RNAs (ncRNAs) have emerged as essential post-transcriptional regulators in bacteria. Nearly all important physiological and stress responses are modulated by ncRNA regulators, such as riboswitches, trans-acting small RNAs (sRNAs), and cis-antisense RNAs.
Keunsub Lee, Kan Wang
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Specificity patterns of Agrobacterium tumefaciens phages
Archiv f�r Mikrobiologie, 1970Lysogeny was not detected in 10 strains of A. tumefaciens by plating techniques or ultra-violet induction. Fifteen phages were isolated from raw sewage against 13 cultures of A. tumefaciens and purified by single-plaque selections. No phage lysed all of the strains of A.
R. J. Boyd+2 more
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THE QUANTITATIVE DETERMINATION OF THE INFECTIVITY OF AGROBACTERIUM TUMEFACIENS
American Journal of Botany, 1965A bioassay relating number of Agrobacterium tumefaciens cells in the inoculum quantitatively to the number of crown‐gall tumors initiated on primary pinto bean leaves is described. Variability in estimation of infectious titers by this assay is similar to that observed in comparable plant virus assays, most determinations showing standard errors of 20%
James A. Lippincott, Gary T. Heberlein
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l-Sorbose metabolism in Agrobacterium tumefaciens
Antonie van Leeuwenhoek, 1976The pathway of L-sorbose metabolism in Agrobacterium tumefaciens strain B6 was determined to be: L-sorbose leads to D-glucitol (sorbitol) leads to D-fructose leads to D-fructose-6-phosphate leads to D-glucose-6-phosphate. The reduction of L-sorbose and the oxidation of D-glucitol were mediated by NADPH- and NAD+-linked oxidoreductases, respectively ...
J. De Ley, Karel Kersters, C. Van Keer
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Agrobacterium tumefaciens-Mediated Plant Transformation: A Review
Molecular Biotechnology, 2023Shahnam Azizi-Dargahlou+1 more
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Exploration of Agrobacterium tumefaciens
1992Bacteria of the genus Agrobacterium are soil-borne plant pathogens. Their unique contribution to biology consists of their natural system for delivering DNA into host plants, where it becomes integrated and thereby stabilized in the genome. As a consequence of expression of bacterial DNA in the plants, the normal developmental pattern is changed ...
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Agrobacterium tumefaciens-Mediated Wheat Transformation
Cereal Research Communications, 2003An efficient and reproducible Agrobacterium tumefaciens-mediated system for wheat transformation was developed and genetically transformed wheat plants were produced using precultured immature embryos as the expiant. The embryos were inoculated with a disarmed A.
Haliloglu, K, Baenziger, PS
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