Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
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Phase separation as an emerging regulatory framework in antibody class switching and genome stability. [PDF]
Refaat AM, Shimizu H, Yasuhara T.
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Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
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Canonical and noncanonical Hippo signaling in C. elegans. [PDF]
Huynh L +4 more
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Effects of automated insulin delivery systems on glucose control in subgroups of adults with type 1 diabetes in clinical practice over 2 years in Sweden. [PDF]
Singh R +8 more
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[Automated insulin delivery (AID) systems, physical activity and sports in type 1 diabetes mellitus: joint guidelines of the DDG and ÖDG]. [PDF]
Moser O +19 more
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Measuring health financing vulnerability due to reductions in official development assistance: A conceptual framework with empirical application across 47 African countries. [PDF]
Asamani JA +9 more
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Efficacy and Safety of Open-Source Hybrid Closed-Loop Automated Insulin Delivery in Perioperative Patients. [PDF]
Ma D +20 more
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Hypothermia risk factors in patients with burns during emergency presentations: protocol for a retrospective cohort study. [PDF]
Vayada DD +17 more
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Correction: <i>Stepping into Play: a FIFA decision aid for football participation after childbirth</i>. [PDF]
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