Results 51 to 60 of about 630,543 (264)
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Case Studies in Thermal EngineeringThe gurgling and hiss noise near thermal expansion valve (TXV) in automobile air conditioning gradually become prominent. However, the causes of noise from the flow-induced vibration (FIV) have not been confirmed.
Xin-Gang Zhu +7 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Flows of air, flows of electrons
The Anthropocene ReviewToday’s comprehension of the global climate and other elements of the Earth system is synonymous with the computation of their dynamic processes in digital machinery. Given their central role in assessing the criticality of the planetary- and epoch-scale changes currently underway, it seems crucial to fathom this conceptual and material basis of the ...
openaire +2 more sources
FEBS Open Bio, EarlyView.Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta +7 more
wiley +1 more source
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective The prognosis of glioblastoma (GBM) remains highly unfavorable, largely due to high tumor heterogeneity and an immunosuppressive microenvironment. However, the functional role of PANoptosis in this context is poorly understood. Methods Patients were stratified via K‐means clustering. A risk score model was constructed using prognosis‐
Langfei Tian +6 more
wiley +1 more source
The Effect of Air Pressure on the Technological Process of Grinding in the Grain Hammer Mill
Инженерные технологии и системыIntroduction. The main problem of the grain hammer mill is over-grinding of grain caused by untimely removal of the final product from the grinding chamber.
Petr A. Savinykh +3 more
doaj +1 more source
Airflow Management in Solid Oxide Electrolyzer (SOE) Operation: Performance Analysis
ChemEngineering, 2017 Hydrogen is being studied as a means of energy storage and can be synthetized to store renewable energy and successively used as a fuel for power production or transport purposes.
Linda Barelli +2 more
doaj +1 more source
Arthritis Care &Research, EarlyView.Objective The study aimed to identify symptom‐based predictors of dry eye disease (DED) signs in the Sjögren's International Collaborative Clinical Alliance (SICCA) cohort. Methods We performed a retrospective analysis examining 16 ocular symptoms (most graded 0–4) and artificial tear (AT) use (graded 0–3) as predictors of DED signs (abnormal ocular ...
Pragnya R. Donthineni +7 more
wiley +1 more source
Stem Cell Research & TherapyBackground The therapeutic status of allogeneic stem cell transplantation (allo-SCT) as a post-remission treatment for patients with high-risk acute myeloid leukemia (AML) was well-accepted. However, the optimal treatment for patients with low/favorable-
Wu Ye +7 more
doaj +1 more source