Results 81 to 90 of about 3,341,032 (343)
A comparison of different ventilation strategies for dwellings in terms of airflow rates and airflow paths [PDF]
, 2016 The context of ventilation in Belgian dwellings has changed since the publication of the Belgian standard NBN D 50-001:1991. Due to the higher energy performance of these dwellings, ventilation plays nowadays a more essential role in maintaining a good ...
Caillou, Samuel +2 more
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Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
FEBS Open Bio, EarlyView.Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar +3 more
wiley +1 more source
Air and Water Quality under Pressure in Africa [PDF]
, 2016 The African continent has large untapped environmental resources: together with Latin America and the Caribbean, the continent has the largest reserve of agricultural land, accounting for 80 per cent of the global total.Africa is home to important ...
core
FEBS Open Bio, EarlyView.Excess Ca2+ ions activate the Calcium‐Sensing Receptor (CaSR), which subsequently drives the uptake of excess inorganic phosphate (Pi) via the Pi transporter (Pit−1) in chondrocytes. This mechanism causes a toxic increase in intracellular Pi concentration, ultimately leading to chondrocyte apoptosis and pathological mineralization. Excess extracellular
Sachie Nakatani +7 more
wiley +1 more source
SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Simple traffic measures significantly reduce the exposure of primary school children to NO2 [PDF]
, 2019 According to Public Health England (PHE, 2019) air quality is the largest environmental health risk in the UK; long-term exposure to air pollution claims some 28-36,000 premature deaths each year.
Cameron, Valerie +3 more
core
FEBS Open Bio, EarlyView.This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso +4 more
wiley +1 more source
FEBS Open Bio, EarlyView.KLK7, a tissue kallikrein‐related peptidase, is elevated in advanced colorectal cancer and associated with shorter survival. High KLK7 levels in ascites correlate with peritoneal metastasis. In mice, KLK7 overexpression increases metastasis. In vitro, KLK7 enhances cancer cell proliferation, migration, adhesion, and spheroid formation, driving ...
Yosr Z. Haffani +6 more
wiley +1 more source
AtmosphereThe Model for Prediction Across Scales-Atmosphere (MPAS) was used to simulate meteorological conditions for a two-week winter episode during 10–23 January 2013, and a two-week summer episode during 18–31 July 2016, using both as a global model and a ...
Kemal Gürer +3 more
doaj +1 more source