Results 11 to 20 of about 6,425 (174)

A bacterial tungsten-containing aldehyde oxidoreductase forms an enzymatic decorated protein nanowire [PDF]

Science Advances, 2023
Abstract Aldehyde oxidoreductases (AOR) are tungsten enzymes catalysing the oxidation of many different aldehydes to the corresponding carboxylic acids. In contrast to other known AORs, the enzyme from the denitrifying betaproteobacterium Aromatoleum aromaticum (AOR
Agnieszka Winiarska, Fidel Ramírez-Amador, Dominik Hege, Yvonne Gemmecker, Simone Prinz, Georg Hochberg, Johann Heider, Maciej Szaleniec, Jan Michael Schuller   +8 more
openaire   +7 more sources

Identification of crucial amino acids in mouse aldehyde oxidase 3 that determine substrate specificity. [PDF]

PLoS ONE, 2013
In order to elucidate factors that determine substrate specificity and activity of mammalian molybdo-flavoproteins we performed site directed mutagenesis of mouse aldehyde oxidase 3 (mAOX3).
Martin Mahro, Natércia F Brás, Nuno M F S A Cerqueira, Christian Teutloff, Catarina Coelho, Maria João Romão, Silke Leimkühler   +6 more
doaj   +3 more sources

Highly stable and reusable immobilized formate dehydrogenases: Promising biocatalysts for in situ regeneration of NADH [PDF]

Beilstein Journal of Organic Chemistry, 2016
This study aimed to prepare robust immobilized formate dehydrogenase (FDH) preparations which can be used as effective biocatalysts along with functional oxidoreductases, in which in situ regeneration of NADH is required.
Barış Binay, Dilek Alagöz, Deniz Yildirim, Ayhan Çelik, S. Seyhan Tükel   +4 more
doaj   +2 more sources

Purification and characterization of a benzylviologen-linked, tungsten-containing aldehyde oxidoreductase from Desulfovibrio gigas [PDF]

Journal of Bacteriology, 1995
Desulfovibrio gigas NCIMB 9332 cells grown in ethanol-containing medium with 0.1 microM tungstate contained a benzylviologen-linked aldehyde oxidoreductase. The enzyme was purified to electrophoretic homogeneity and found to be a homodimer with a subunit M(r) of 62,000. It contained 0.68 +/- 0.08 W, 4.8 Fe, and 3.2 +/- 0.2 labile S per subunit.
HENSGENS, CMH, HAGEN, WR, HANSEN, TA
core   +7 more sources

Nicotinoprotein [NAD(P)‐containing] alcohol/aldehyde oxidoreductases [PDF]

European Journal of Biochemistry, 1993
Extracts of Gram‐positive bacteria like Rhodococcus rhodochrous, Rhodococcus erythropolis and Amycolatopsis methanolica, but not those of several Gram‐negative ones, showed dehydrogenase activity for ethanol as well as for methanol when 4‐nitroso‐N, N‐dimethylaniline (NDMA) was used as electron acceptor.
P W, Van Ophem, J, Van Beeumen, J A, Duine   +2 more
openaire   +4 more sources

Coupled immobilized bi-enzymatic flow reactor employing cofactor regeneration of NAD+ using a thermophilic aldehyde dehydrogenase and lactate dehydrogenase [PDF]

, 2023
he use of enzymes in biochemical processes is of interest due to their ability to work under mild conditions while attaining high reaction rates. A limitation in the use of enzymes such as oxidoreductases on a large scale lies with their requirement for ...
Xinxin Xiao (4849261), EDMOND MAGNER (12353191), Serguei Belochapkine (2119438), Kim Leah Shortall (14716204), Simin Arshi (14127330), Simon Bendl (14013256), DENISE DEMURTAS (12353140), TEWFIK SOULIMANE (12351892)   +7 more
core   +4 more sources

Electrocatalytic Aldehyde Oxidation by a Tungsten Dependent Aldehyde Oxidoreductase from Aromatoleum Aromaticum

Chemistry – A European Journal, 2023
AbstractIn contrast to their molybdenum dependent relatives, tungsten enzymes operate at significantly lower redox potentials, and in some cases they can carry out reversible redox transformations of their substrates and products. Still, the electrochemical properties of W enzymes have received much less attention than their Mo relatives.
Palraj Kalimuthu, Dominik Hege, Agnieszka Winiarska, Yvonne Gemmecker, Maciej Szaleniec, Johann Heider, Paul V. Bernhardt   +6 more
openaire   +4 more sources

Electron Microscopic Analysis and Structural Characterization of Novel NADP(H)-Containing Methanol: N,N'-Dimethyl-4-Nitrosoaniline Oxidoreductases from the Gram-Positive Methylotrophic Bacteria Amycolatopsis methanolica and Mycobacterium gastri MB19 [PDF]

, 1993
The quaternary protein structure of two methanol:N,N'-dimethyl-4-nitrosoaniline (NDMA) oxidoreductases purified from Amycolatopsis methanolica and Mycobacterium gastri MB19 was analyzed by electron microscopy and image processing.
Bruggen, Ernst F.J. van,, Bystrykh, Leonid V, Bystrykh, Leonid V.,, Bruggen, Ernst F.J. van, Govorukhina, Natalya I.,, Duine, Johannis A., Vonck, Janet,, Dijkhuizen, Lubbert,, Dijkhuizen, Lubbert, Arfman, Nico,, Dijkhuizen, Lubbert; id_orcid, Van Beeumen, Jozef, Beeumen, Jozef van, Duine, Johannis A.,, Arfman, Nico, Bystrykh, Leonid V., van Bruggen, Ernst FJ, Govorukhina, Natalya I, Samyn, Bart, Duine, Johannis A, Beeumen, Jozef van,, Govorukhina, Natalya I., Vonck, Janet, Samyn, Bart,   +23 more
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Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase [PDF]

European Journal of Biochemistry, 2004
This work reports on the direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase (DgAOR), a molybdenum enzyme of the xanthine oxidase family that contains three redox‐active cofactors: two [2Fe‐2S] centers and a molybdopterin cytosine dinucleotide cofactor.
Margarida M, Correia dos Santos, Patrícia M P, Sousa, M Lurdes S, Gonçalves, M João, Romão, Isabel, Moura, José J G, Moura   +5 more
openaire   +2 more sources

Membrane electrochemical reactors (MER) for NADH regeneration in HLADH-catalysed synthesis: comparison of effectiveness [PDF]

, 2004
Two membrane electrochemical reactors(MER) were designed and applied to HLADH-catalysed reduction of cyclohexanone to cyclohexanol. The regeneration of the cofactor NADH was ensured electrochemically, using either methyl viologen or a rhodium complex as
Basséguy, Régine, Bergel, Alain, Délécouls-Servat, Karine   +2 more
core   +1 more source