5-Methylcytosine (5mC) in DNA CpG islands is an important epigenetic biomarker for mammalian gene regulation. It is oxidized to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC) by the ten-eleven translocation (TET ...
Ke Bian+9 more
semanticscholar +1 more source
Identity and mechanisms of alkane-oxidizing metalloenzymes from deep-sea hydrothermal vents
Six aerobic alkanotrophs (organism that can metabolize alkanes as their sole carbon source) isolated from deep-sea hydrothermal vents were characterized using the radical clock substrate norcarane to determine the metalloenzyme and reaction mechanism ...
Rachel Narehood Austin+5 more
doaj +1 more source
Modulating the import of medium-chain alkanes in E. coli through tuned expression of FadL [PDF]
BACKGROUND: In recent years, there have been intensive efforts to develop synthetic microbial platforms for the production, biosensing and bio-remediation of fossil fuel constituents such as alkanes.
Akhtar, MK+3 more
core +1 more source
Efficient production of the Nylon 12 monomer ω-aminododecanoic acid methyl ester from renewable dodecanoic acid methyl ester with engineered Escherichia coli [PDF]
The expansion of microbial substrate and product scopes will be an important brick promoting future bioeconomy. In this study, an orthogonal pathway running in parallel to native metabolism and converting renewable dodecanoic acid methyl ester (DAME) via
Aßmann, M+5 more
core +1 more source
Proliferation of hydrocarbon-degrading microbes at the bottom of the Mariana Trench [PDF]
Background: The Mariana Trench is the deepest known site in the Earth’s oceans, reaching a depth of ~ 11,000m 20 at the Challenger Deep. Recent studies reveal that hadal waters harbor distinctive microbial planktonic communities.
A Schirmer+45 more
core +3 more sources
Structural basis for substrate discrimination byE. colirepair enzyme, AlkB
Positive charge on methylated nucleotides is a prime criterion for substrate recognition byE. coliAlkB.
Namrata Jayanth+3 more
openaire +4 more sources
Enhanced crude oil depletion by constructed bacterial consortium comprising bioemulsifier producer and petroleum hydrocarbon degraders. [PDF]
The aim of this work was to study the production of bioemulsifier by Rhodococcus erythropolis OSDS1, and the improvement of crude oil depletion efficiency using a consortium of petroleum hydrocarbon degraders and OSDS1.
Chakraborty, Romy+4 more
core
Alkbh8 Regulates Selenocysteine-Protein Expression to Protect against Reactive Oxygen Species Damage [PDF]
Environmental and metabolic sources of reactive oxygen species (ROS) can damage DNA, proteins and lipids to promote disease. Regulation of gene expression can prevent this damage and can include increased transcription, translation and post translational
Begley, Thomas J.+8 more
core +3 more sources
Functional characterization of genes involved in alkane oxidation by Pseudomonas aeruginosa [PDF]
Most clinical isolates identified as Pseudomonas aeruginosa grow on long-chain n-alkanes, while environmental P. aeruginosa isolates often grow on medium- as well as long-chain n-alkanes.
Smits, Theo+2 more
core
Microbial Degradation of Petroleum Hydrocarbons by Acinetobacter sp. BZ-15,Isolated from Contaminated Soil [PDF]
本研究旨在从土壤中筛选高效石油烃降解菌株,并对其系统分类和降解特性进行研究,为石油污染的原位修复奠定基础.该研究从滨州油井溢油污染土壤样品中分离得到一株高效石油烃降解菌株BZ-15,对菌株BZ-15进行形态观察、16S r RNA基因序列分析及系统发育树分析;对该菌株的生长特性进行研究;通过GC-MS分析其对原油组分中不同碳原子饱和烃的降解特性;同时研究吐温-20对其生长及降解特性的影响;对该菌株中的烷烃羟化酶基因alk M进行了克隆.结果表明,菌株BZ-15为不动杆菌属(Acinetobacter ...
刘玉华+2 more
core +1 more source