Results 191 to 200 of about 6,739 (216)

Computational Insights into the Non-Heme Diiron Alkane Monooxygenase Enzyme AlkB: Electronic Structures, Dioxygen Activation, and Hydroxylation Mechanism of Liquid Alkanes.

Inorganic Chemistry
Alkane monooxygenase (AlkB) is a membrane-spanning metalloenzyme that catalyzes the terminal hydroxylation of straight-chain alkanes involved in the microbially mediated degradation of liquid alkanes.
Yue Wang, Yongjun Liu
semanticscholar   +1 more source

High expression of AlkB homolog 5 suppresses the progression of non‐small cell lung cancer by facilitating ferroptosis through m6A demethylation of SLC7A11

Environmental Toxicology
Non‐small cell lung cancer (NSCLC) is a prevailing LC characterized by poor outcomes. AlkB homolog 5 (ALKBH5) functions as a tumor suppressor in several cancers. This study delved into the role of ALKBH5 in NSCLC development.
Zhangzhou Huang, Gen Lin, Yaping Hong, Lihong Weng, Kai Zhu, Zhuang Wu   +5 more
semanticscholar   +1 more source

Selective Inhibitors of AlkB Family of Nucleic Acid Demethylases

Biochemistry, 2019
The α-ketoglutarate-dependent (AlkB) superfamily of FeII/2-oxoglutarate (2-OG)-dependent dioxygenases consists of a unique class of nucleic acid repair enzymes that reversibly remove alkyl substituents from nucleobases through oxidative dealkylation.
Li-Jun Xie, Li Liu, Liang Cheng
openaire   +3 more sources

A novel, divergent alkane monooxygenase (alkB) clade involved in crude oil biodegradation.

Environmental Microbiology Reports, 2021
Alkanes are ubiquitous in marine ecosystems and originate from diverse sources ranging from natural oil seeps to anthropogenic inputs and biogenic production by cyanobacteria. Enzymes that degrade cyanobacterial alkanes (typically C15-C17 compounds) such
S. Karthikeyan, J. Hatt, Minjae Kim, J. Spain, M. Huettel, J. Kostka, K. Konstantinidis   +6 more
semanticscholar   +1 more source

Genetic Diversity at alkB Locus in Brucella abortus

Journal of Veterinary Medicine, Series B, 2003
SummaryDNA polymorphism of the alkB gene, a DNA repair gene, was assessed by PCR on Brucella abortus biovars 1 (strains 99, S19, 45/20, RB51 and 2308), 3 (Tulya strain), 5 (B3196 strain) and 6 (870 strain). A DNA repetitive element, named IS711, was detected in all studied biovars 1 and its complete nucleotide sequence was determined. We found that the
Michele Muscillo, G. La Rosa, Rosanna Adone, Franco Ciuchini, Paolo Pasquali, Cinzia Marianelli   +5 more
openaire   +3 more sources

Molecular cloning and characterization of the alkB gene of Escherichia coli

Molecular and General Genetics MGG, 1985
Using methods of in vitro recombination we constructed hybrid plasmids that can suppress the increased methylmethane sulfonate sensitivity caused by alkB mutation. Since the cloned DNA fragment was mapped at 47 min on the Escherichia coli K12 genetic map, an area where the alkB gene is located, we concluded that the cloned DNA fragment contains the ...
Mutsuo Sekiguchi, Hiroko Kataoka
openaire   +2 more sources

Attenuation of petroleum hydrocarbon fractions using rhizobacterial isolates possessing alkB, C23O, and nahR genes for degradation of n-alkane and aromatics

Journal of Environmental Science and Health. Part A: Toxic/Hazardous Substances and Environmental Engineering, 2021
This work assessed the catabolic versatility of functional genes in hydrocarbon-utilizing bacteria obtained from the rhizosphere of plants harvested in aged polluted soil sites in Ogoni and their attenuation efficacy in a bioremediation study ...
J. E. Agbaji, E. Nwaichi, G. Abu
semanticscholar   +1 more source

alkB homologs in thermophilic bacteria of the genus Geobacillus

Molecular Biology, 2008
Screening of alkane hydroxylase genes (alkB) was performed in the thermophilic aerobic bacteria of the genus Geobacillus. Total DNA was extracted from the biomass of 11 strains grown on the mixture of saturated C10-C20 hydrocarbons, PCR amplification of fragments of alkB genes was performed with degenerate oligonucleotide primers, PCR products were ...
Tamara N. Nazina, A. B. Poltaraus, A. V. Mashukova, T. A. Rodionova, E. M. Mikhailova, Tatyana P. Tourova, A. N. Ekimov   +6 more
openaire   +3 more sources

A Fix on AlkB

Science of Aging Knowledge Environment, 2002
After almost 2 decades of scrutiny, a mysterious DNA-repair protein has bared its inner workings. By mixing a set of ingredients never before known to be used by enzymes with that function, two research groups have deduced its mechanism. The studies reveal that the protein works differently from other DNA fix-it proteins and repairs defects in a single
openaire   +2 more sources

Expression of Rhodococcus opacus alkB genes in anhydrous organic solvents

Journal of Bioscience and Bioengineering, 2008
Rhodococcus opacus B-4 is a benzene-tolerant bacterium which was isolated from a gasoline-contaminated soil sample. We previously demonstrated that this organism was able to survive and exhibit biocatalytic activity in anhydrous organic solvents for at least 5 d.
Kohsuke Honda, Hisao Ohtake, Takeshi Omasa, Yuka Sameshima, Junichi Kato   +4 more
openaire   +3 more sources