Results 231 to 240 of about 10,817 (260)

Aberrant activity of the DNA repair enzyme AlkB

Journal of Inorganic Biochemistry, 2004
Escherichia coli AlkB is a DNA/RNA repair enzyme containing a mononuclear Fe(II) site that couples the oxidative decomposition of alpha-ketoglutarate (alphaKG) to the hydroxylation of 1-methyladenine or 3-methylcytosine lesions in DNA or RNA, resulting in release of formaldehyde and restoration of the normal bases.
Robert P. Hausinger, Michael Feig, Timothy F. Henshaw   +2 more
openaire   +3 more sources

Alkane biodegradation in Pseudomonas aeruginosa strains isolated from a polluted zone: identification of alkB and alkB-related genes

Research in Microbiology, 2002
Pseudomonas aeruginosa strains that grow on crude oil as the sole source of carbon and energy were isolated from an environment in Morocco polluted by petroleum refinery effluents. The twenty isolates grew on saturated alkanes from C12 to C22. Three of the isolates were also able to grow on low molecular weight C6 to C10 n-alkanes, but the other 17 ...
Nicole Desnoues, Claudine Elmerich, Abdelhaq Belhaj   +2 more
openaire   +3 more sources

AlkB demethylases flip out in different ways

DNA Repair, 2008
Aberrant methylations in DNA are repaired by base excision repair (BER) and direct repair by a methyltransferase or by an oxidative demethylase of the AlkB type. Yang et al. [Nature 452 (2008) 961-966] have now solved the crystal structure of AlkB and human AlkB homolog 2 (hABH2) in complex with DNA using an ingenious crosslinking strategy to stabilize
Vivi Talstad, Cathrine Broberg Vågbø, Geir Slupphaug, Ottar Sundheim, Hans E. Krokan   +4 more
openaire   +2 more sources

High expression of AlkB homolog 5 suppresses the progression of non‐small cell lung cancer by facilitating ferroptosis through m6A demethylation of SLC7A11

Environmental Toxicology
Non‐small cell lung cancer (NSCLC) is a prevailing LC characterized by poor outcomes. AlkB homolog 5 (ALKBH5) functions as a tumor suppressor in several cancers. This study delved into the role of ALKBH5 in NSCLC development.
Zhangzhou Huang, Gen Lin, Yaping Hong, Lihong Weng, Kai Zhu, Zhuang Wu   +5 more
semanticscholar   +1 more source

Computational Insights into the Non-Heme Diiron Alkane Monooxygenase Enzyme AlkB: Electronic Structures, Dioxygen Activation, and Hydroxylation Mechanism of Liquid Alkanes.

Inorganic Chemistry
Alkane monooxygenase (AlkB) is a membrane-spanning metalloenzyme that catalyzes the terminal hydroxylation of straight-chain alkanes involved in the microbially mediated degradation of liquid alkanes.
Yue Wang, Yongjun Liu
semanticscholar   +1 more source

A novel, divergent alkane monooxygenase (alkB) clade involved in crude oil biodegradation.

Environmental Microbiology Reports, 2021
Alkanes are ubiquitous in marine ecosystems and originate from diverse sources ranging from natural oil seeps to anthropogenic inputs and biogenic production by cyanobacteria. Enzymes that degrade cyanobacterial alkanes (typically C15-C17 compounds) such
S. Karthikeyan, J. Hatt, Minjae Kim, J. Spain, M. Huettel, J. Kostka, K. Konstantinidis   +6 more
semanticscholar   +1 more source

Selective Inhibitors of AlkB Family of Nucleic Acid Demethylases

Biochemistry, 2019
The α-ketoglutarate-dependent (AlkB) superfamily of FeII/2-oxoglutarate (2-OG)-dependent dioxygenases consists of a unique class of nucleic acid repair enzymes that reversibly remove alkyl substituents from nucleobases through oxidative dealkylation.
Li-Jun Xie, Li Liu, Liang Cheng
openaire   +3 more sources

Genetic Diversity at alkB Locus in Brucella abortus

Journal of Veterinary Medicine, Series B, 2003
SummaryDNA polymorphism of the alkB gene, a DNA repair gene, was assessed by PCR on Brucella abortus biovars 1 (strains 99, S19, 45/20, RB51 and 2308), 3 (Tulya strain), 5 (B3196 strain) and 6 (870 strain). A DNA repetitive element, named IS711, was detected in all studied biovars 1 and its complete nucleotide sequence was determined. We found that the
Michele Muscillo, G. La Rosa, Rosanna Adone, Franco Ciuchini, Paolo Pasquali, Cinzia Marianelli   +5 more
openaire   +3 more sources

Molecular cloning and characterization of the alkB gene of Escherichia coli

Molecular and General Genetics MGG, 1985
Using methods of in vitro recombination we constructed hybrid plasmids that can suppress the increased methylmethane sulfonate sensitivity caused by alkB mutation. Since the cloned DNA fragment was mapped at 47 min on the Escherichia coli K12 genetic map, an area where the alkB gene is located, we concluded that the cloned DNA fragment contains the ...
Mutsuo Sekiguchi, Hiroko Kataoka
openaire   +2 more sources

Attenuation of petroleum hydrocarbon fractions using rhizobacterial isolates possessing alkB, C23O, and nahR genes for degradation of n-alkane and aromatics

Journal of Environmental Science and Health. Part A: Toxic/Hazardous Substances and Environmental Engineering, 2021
This work assessed the catabolic versatility of functional genes in hydrocarbon-utilizing bacteria obtained from the rhizosphere of plants harvested in aged polluted soil sites in Ogoni and their attenuation efficacy in a bioremediation study ...
J. E. Agbaji, E. Nwaichi, G. Abu
semanticscholar   +1 more source