Results 231 to 240 of about 10,203 (251)
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Research in Microbiology, 2002
Pseudomonas aeruginosa strains that grow on crude oil as the sole source of carbon and energy were isolated from an environment in Morocco polluted by petroleum refinery effluents. The twenty isolates grew on saturated alkanes from C12 to C22. Three of the isolates were also able to grow on low molecular weight C6 to C10 n-alkanes, but the other 17 ...
Abdelhaq, Belhaj +2 more
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Pseudomonas aeruginosa strains that grow on crude oil as the sole source of carbon and energy were isolated from an environment in Morocco polluted by petroleum refinery effluents. The twenty isolates grew on saturated alkanes from C12 to C22. Three of the isolates were also able to grow on low molecular weight C6 to C10 n-alkanes, but the other 17 ...
Abdelhaq, Belhaj +2 more
openaire +4 more sources
Oxidative demethylation by Escherichia coli AlkB directly reverts DNA base damage
Nature, 2002Methylating agents generate cytotoxic and mutagenic DNA damage. Cells use 3-methyladenine-DNA glycosylases to excise some methylated bases from DNA, and suicidal O(6)-methylguanine-DNA methyltransferases to transfer alkyl groups from other lesions onto a cysteine residue.
Robert P Hausinger
exaly +3 more sources
Aberrant activity of the DNA repair enzyme AlkB
Journal of Inorganic Biochemistry, 2004Escherichia coli AlkB is a DNA/RNA repair enzyme containing a mononuclear Fe(II) site that couples the oxidative decomposition of alpha-ketoglutarate (alphaKG) to the hydroxylation of 1-methyladenine or 3-methylcytosine lesions in DNA or RNA, resulting in release of formaldehyde and restoration of the normal bases.
Timothy F, Henshaw +2 more
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Science of Aging Knowledge Environment, 2002
After almost 2 decades of scrutiny, a mysterious DNA-repair protein has bared its inner workings. By mixing a set of ingredients never before known to be used by enzymes with that function, two research groups have deduced its mechanism. The studies reveal that the protein works differently from other DNA fix-it proteins and repairs defects in a single
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After almost 2 decades of scrutiny, a mysterious DNA-repair protein has bared its inner workings. By mixing a set of ingredients never before known to be used by enzymes with that function, two research groups have deduced its mechanism. The studies reveal that the protein works differently from other DNA fix-it proteins and repairs defects in a single
openaire +1 more source
Selective Inhibitors of AlkB Family of Nucleic Acid Demethylases
Biochemistry, 2019The α-ketoglutarate-dependent (AlkB) superfamily of FeII/2-oxoglutarate (2-OG)-dependent dioxygenases consists of a unique class of nucleic acid repair enzymes that reversibly remove alkyl substituents from nucleobases through oxidative dealkylation.
Li-Jun Xie, Li Liu, Liang Cheng
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alkB homologs in thermophilic bacteria of the genus Geobacillus
Molecular Biology, 2008Screening of alkane hydroxylase genes (alkB) was performed in the thermophilic aerobic bacteria of the genus Geobacillus. Total DNA was extracted from the biomass of 11 strains grown on the mixture of saturated C10-C20 hydrocarbons, PCR amplification of fragments of alkB genes was performed with degenerate oligonucleotide primers, PCR products were ...
T P, Turova +6 more
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Genetic Diversity at alkB Locus in Brucella abortus
Journal of Veterinary Medicine, Series B, 2003SummaryDNA polymorphism of the alkB gene, a DNA repair gene, was assessed by PCR on Brucella abortus biovars 1 (strains 99, S19, 45/20, RB51 and 2308), 3 (Tulya strain), 5 (B3196 strain) and 6 (870 strain). A DNA repetitive element, named IS711, was detected in all studied biovars 1 and its complete nucleotide sequence was determined. We found that the
C, Marianelli +5 more
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AlkB and Its Homologues – DNA Repair and Beyond
2015AlkB is an Fe(ii)/2-oxoglutarate-dependent dioxygenase that is part of the adaptive response to alkylating agents in Escherichia coli. AlkB hydroxylates a wide variety of alkylated DNA bases producing unstable intermediates which decompose to restore the non-alkylated bases. Homologues exist in other bacteria, metazoa (e.g.
Tina A. Müller, Robert P. Hausinger
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Molecular cloning and characterization of the alkB gene of Escherichia coli
Molecular and General Genetics MGG, 1985Using methods of in vitro recombination we constructed hybrid plasmids that can suppress the increased methylmethane sulfonate sensitivity caused by alkB mutation. Since the cloned DNA fragment was mapped at 47 min on the Escherichia coli K12 genetic map, an area where the alkB gene is located, we concluded that the cloned DNA fragment contains the ...
H, Kataoka, M, Sekiguchi
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