Results 241 to 250 of about 10,817 (260)
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alkB homologs in thermophilic bacteria of the genus Geobacillus
Molecular Biology, 2008Screening of alkane hydroxylase genes (alkB) was performed in the thermophilic aerobic bacteria of the genus Geobacillus. Total DNA was extracted from the biomass of 11 strains grown on the mixture of saturated C10-C20 hydrocarbons, PCR amplification of fragments of alkB genes was performed with degenerate oligonucleotide primers, PCR products were ...
Tamara N. Nazina +6 more
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Science of Aging Knowledge Environment, 2002
After almost 2 decades of scrutiny, a mysterious DNA-repair protein has bared its inner workings. By mixing a set of ingredients never before known to be used by enzymes with that function, two research groups have deduced its mechanism. The studies reveal that the protein works differently from other DNA fix-it proteins and repairs defects in a single
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After almost 2 decades of scrutiny, a mysterious DNA-repair protein has bared its inner workings. By mixing a set of ingredients never before known to be used by enzymes with that function, two research groups have deduced its mechanism. The studies reveal that the protein works differently from other DNA fix-it proteins and repairs defects in a single
openaire +2 more sources
Expression of Rhodococcus opacus alkB genes in anhydrous organic solvents
Journal of Bioscience and Bioengineering, 2008Rhodococcus opacus B-4 is a benzene-tolerant bacterium which was isolated from a gasoline-contaminated soil sample. We previously demonstrated that this organism was able to survive and exhibit biocatalytic activity in anhydrous organic solvents for at least 5 d.
Kohsuke Honda +4 more
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AlkB and Its Homologues – DNA Repair and Beyond
2015AlkB is an Fe(ii)/2-oxoglutarate-dependent dioxygenase that is part of the adaptive response to alkylating agents in Escherichia coli. AlkB hydroxylates a wide variety of alkylated DNA bases producing unstable intermediates which decompose to restore the non-alkylated bases. Homologues exist in other bacteria, metazoa (e.g.
Robert P. Hausinger, Tina A. Müller
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Journal of the American Chemical Society
Alkane monooxygenase (AlkB) is the dominant enzyme that catalyzes the oxidation of liquid alkanes in the environment. Two recent structural models derived from cryo-electron microscopy (cryo-EM) reveal an unusual active site: a histidine-rich center that
Clorice R. Reinhardt +10 more
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Alkane monooxygenase (AlkB) is the dominant enzyme that catalyzes the oxidation of liquid alkanes in the environment. Two recent structural models derived from cryo-electron microscopy (cryo-EM) reveal an unusual active site: a histidine-rich center that
Clorice R. Reinhardt +10 more
semanticscholar +1 more source
A DFT Study of Nucleobase Dealkylation by the DNA Repair Enzyme AlkB
The Journal of Physical Chemistry B, 2009Oxidative dealkylation is a unique mechanistic pathway found in the alpha-ketoglutarate-Fe(II)-dependent AlkB family of enzymes to remove the alkylation damage to DNA bases and regenerate nucleobases to their native state. The B3LYP density functional combined with a self-consistent reaction field was used to explore the triplet, quintet, and septet ...
Haining Liu, Jorge Llano, James W. Gauld
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Structural and mechanistic insight into alkane hydroxylation by Pseudomonas putida AlkB
Biochemical Journal, 2014Pseudomonas putida GPo1 alkane hydroxylase (AlkB) is an integral membrane protein that catalyses the hydroxylation of medium-chain alkanes (C3–C12). 1-Octyne irreversibly inhibits this non-haem di-iron mono-oxygenase under turnover conditions, suggesting that it acts as a mechanism-based inactivator.
Alonso, Hernan +7 more
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AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli
Nature, 2002The bacterial AlkB protein is known to be involved in cellular recovery from alkylation damage; however, the function of this protein remains unknown. AlkB homologues have been identified in several organisms, including humans, and a recent sequence alignment study has suggested that these proteins may belong to a superfamily of 2-oxoglutarate ...
Erling Seeberg +2 more
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Evaluation of the Escherichia coli HK82 and BS87 strains as tools for AlkB studies
DNA Repair, 2016Within a decade the family of AlkB dioxygenases has been extensively studied as a one-protein DNA/RNA repair system in Escherichia coli but also as a group of proteins of much wider functions in eukaryotes. Two strains, HK82 and BS87, are the most commonly used E. coli strains for the alkB gene mutations.
D. Mielecki +7 more
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