Results 31 to 40 of about 53,713 (267)
FEBS Letters, EarlyView.We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee +3 more
wiley +1 more source
Tailoring strength and ductility in additively manufactured 410 ferritic stainless steel
Materials Research ExpressIn this work, the microstructure and mechanical properties of as-printed and heat-treated additively manufactured ferritic stainless steels (FSS) (AM410SS) with high strength is investigated.
Harveen Bongao +4 more
doaj +1 more source
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Enhanced precipitation strengthening of multi-principal element alloys by κ- and B2-phases
Materials & Design, 2021 Discoveries of new multi-principal element alloys (MPEAs) with outstanding performance have been challenging due to the large complexity of the compositional space.
Fabian Kies +4 more
doaj +1 more source
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
FEBS Letters, EarlyView.An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley +1 more source
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Low-loss phase-change material-based programmable mode converter for photonic computing
NPG Asia MaterialsPhase-change materials (PCMs)-based integrated photonic memory offers a viable pathway for the development of neuromorphic computing chips. The sizable optical contrast in the telecom band between amorphous and crystalline phases of PCM, in particular ...
Xueyang Shen +5 more
doaj +1 more source
FFT-based interface decohesion modelling by a nonlocal interphase
Advanced Modeling and Simulation in Engineering Sciences, 2018 In this paper, two nonlocal approaches to incorporate interface damage in fast Fourier transform (FFT) based spectral methods are analysed. In FFT based methods, the discretisation is generally non-conforming to the interfaces and hence interface ...
Luv Sharma +4 more
doaj +1 more source
Frontiers in Materials, 2020 The solidification microstructure of Al0.8CrCuFeNi2 high entropy alloy consists of primary fcc dendrites decorated with interdendritic bcc and B2 phases.
Julien Zollinger +3 more
doaj +1 more source