Results 61 to 70 of about 1,497 (165)
, 2022 For each of the two conditions, i.e. without (BBTV-alpha) and with (BBTV+alpha) alphasatellite, Illumina 75 nt reverse reads of the three biological replicates (leaf tissues of three plants) were combined and mapped simultaneously onto the reference ...
Huong Cam Chu (12407572) +10 more
core +1 more source
Use of the cotton leaf curl Multan alphasatellite as a silencing or expression vector
Acta virologica, 2019 Alphasatellites, formerly known as DNA 1, are a satellite-like components associated with begomoviruses (the family Geminiviridae) that require betasatellite for symptom induction but depend on DNA-A for systemic movement. We have converted alphasatellite into gene-silencing vector (modified alphasatellite (∆DNA 1)) by deleting its A-rich region that ...
M S, Shahid, S, Mansoor, R W, Briddon
openaire +3 more sources
Impact of DRC alphasatellite on viral DNA concentrations in plants and aphids.
, 2022 Viral DNA concentrations for each BBTV component (C, M, N, R, S, U3) and alphasatellite (a) and for total helper virus without (BBTV) or with (BBTVa) alphasatellite were measured by quantitative PCR using equal amounts of total DNA in (A) plants without (
Huong Cam Chu (12407572) +10 more
core +1 more source
Construction of BBTV and alphasatellite infectious clones.
, 2022 The complete sequences of BBTV components and alphasatellite from the DRC-2016 aphids (sample JGF-5, S2 Fig) were cloned as partial (DNAs C, M_v1, N, S_v1, U3_v1) or complete (DNA-R) dimers first in pBluescript SK(-) (A) and then in pCambia2300 (B).
Huong Cam Chu (12407572) +10 more
core +1 more source
, 2022 Transcription rates of viral mRNAs were calculated by dividing mRNA counts in reads per million by viral DNA loads (measured by qPCR) and by mRNA unit sizes in nucleotides (alphasatellite = 902 nts; C = 549 nts; M = 526 nts; N = 511 nts; R = 883 nts; S =
Huong Cam Chu (12407572) +10 more
core +1 more source
Molecular Plant-Microbe Interactions, 2011 Begomoviruses (family Geminiviridae) are single-stranded DNA viruses transmitted by the whitefly Bemisia tabaci. Many economically important diseases in crops are caused by begomoviruses, particularly in tropical and subtropical environments.
Imran Amin +8 more
doaj +1 more source
Origins and features of alphasatellite and betasatellite clones obtained.
, 2016 Origins and features of alphasatellite and betasatellite clones obtained.
Rob W. Briddon (143034) +6 more
core +1 more source
Phylogenetic analysis of DRC alphasatellite.
, 2022 A maximum likelihood phylogenetic tree of complete nucleotide sequences of the two isolates of DRC alphasatellite and all isolates of other alphasatellites associated with helper viruses of the families Nanoviridae, Metaxiviridae and Geminiviridae is ...
Huong Cam Chu (12407572) +10 more
core +1 more source
Journal of Materials Research and Technology, 2021 The development of portable and sensitive biosensors for the label-free detection of DNA has influenced fundamental biological research as well as advanced applications.
Amna Rafiq +10 more
doaj +1 more source
, 2022 (A) Duplex PCR analysis with BBTV DNA-R and DRC alphasatellite (α) specific primers of total DNA from symptomatic leaves of N. benthamiana agroinoculated with six BBTV components (lanes 3–6), six BBTV components and alphasatellite (lanes 7–10) or empty ...
Huong Cam Chu (12407572) +10 more
core +1 more source