Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Sugar and ethanol production potential of sweet potato (Ipomoea batatas) as an alternative energy feedstock: processing and physicochemical characterizations. [PDF]
Salelign K, Duraisamy R.
europepmc +1 more source
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young +7 more
wiley +1 more source
The short-circuit current of renewable energy sources (RESs) is limited in amplitude, controlled in phase, and accompanied by complex harmonic components. The unique fault characteristics of RESs and the influence of line distributed capacitance, results
Tao Zheng, Ruozhu Zhang, Yilin Sun
doaj +1 more source
Beyond mitochondria: Alternative energy-producing pathways from all strata of life. [PDF]
Auger C +3 more
europepmc +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Pilot Protection Based on Amplitude Comparison for Renewable Power Teed Lines
Ke Jia +6 more
doaj +1 more source
Evaluation of waste plastic and waste cooking oil as a potential alternative fuel in diesel engine
The generation of plastic waste and waste cooking oil is a serious environmental concern because of worldwide waste disposal issues. At the same time, increasing demand and contemporary geopolitics make fossil fuels a significant worldwide problem.
Amit Kumar +2 more
doaj +1 more source
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
Modern Hybrid Rye, as an Alternative Energy Source for Broiler Chickens, Improves the Absorption Surface of the Small Intestine Depending on the Intestinal Part and Xylanase Supplementation. [PDF]
Donaldson J +11 more
europepmc +1 more source

