Results 101 to 110 of about 2,389,287 (216)

Modelling stem cell differentiation related processes—A practical overview for biologists

FEBS Letters, EarlyView.
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar, Xinqi Yan, Lucas Jansen Klomp, Stefano Schivo, Janine N. Post   +4 more
wiley   +1 more source

Automatic classification of experimental models in biomedical literature to support searching for alternative methods to animal experiments. [PDF]

J Biomed Semantics, 2023
Neves M, Klippert A, Knöspel F, Rudeck J, Stolz A, Ban Z, Becker M, Diederich K, Grune B, Kahnau P, Ohnesorge N, Pucher J, Schönfelder G, Bert B, Butzke D.   +14 more
europepmc   +1 more source

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Development and Comparison of Alternative Methods for the Purification of Adalimumab Directly from Harvested Cell Culture Fluid. [PDF]

Food Technol Biotechnol, 2023
Vaskó D, Domján J, Szűcs B, Bakk L, Hajdinák P, Marosi G, Nagy ZK, Hirsch E, Fehér C.   +8 more
europepmc   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

Differences in characteristics and use of complementary and alternative methods for coping with endometriosis-associated acyclic pelvic pain across adolescence and adulthood. [PDF]

Front Reprod Health, 2023
Mongiovi JM, Wallace B, Goodwin M, Vitonis AF, Karevicius S, Shafrir AL, Sasamoto N, DiVasta AD, Sieberg CB, Terry KL, Missmer SA.   +10 more
europepmc   +1 more source

Mixed‐class J‐domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly

FEBS Letters, EarlyView.
Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley   +1 more source

Alternative Methods as Tools for Obesity Research: In Vitro and In Silico Approaches. [PDF]

Life (Basel), 2022
Pamplona JH, Zoehler B, Shigunov P, Barisón MJ, Severo VR, Erich NM, Ribeiro AL, Horinouchi CDDS, Suzukawa AA, Robert AW, Abud APR, Aguiar AM.   +11 more
europepmc   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Usage of Complementary and Alternative Methods, Lifestyle, and Psychological Variables in Cancer Care. [PDF]

In Vivo, 2023
Hoppe C, Buntzel J, VON Weikersthal LF, Junghans C, Zomorodbakhsch B, Stoll C, Prott FJ, Fuxius S, Micke O, Richter A, Sallmann D, Hubner J.   +11 more
europepmc   +1 more source