Results 181 to 190 of about 87,604,756 (316)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Porcine-derived materials in urology Practical ethical guidance for caring for Muslim and Jewish patients. [PDF]

Can Urol Assoc J
Khan MZ, Ismail A, Kotb A.
europepmc   +1 more source

Why Have Alternatives to Animal Skin Testing Lagged Behind Alternatives to Animal Eye Testing? [PDF]

Journal of Toxicology: Cutaneous and Ocular Toxicology, 1993
openaire   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Towards an in vitro test system for developmental neurotoxicity: a new test method using mouse embryonic stem cells

, 2007
Today, on a large number of chemicals we lack information on their adverse effects on the developing nervous system. Existing in vivo test methods are time consuming, laborious, expensive and require high numbers of laboratory animals. Thus, standardized,
Slawik,B., Seiler,A., Visan,A., Spielmann,H., Hayess,K.   +4 more
core  

Collaboration between Human and Veterinary Research and Medical Experts Could Provide a Balanced Discussion on the Ethics of Using Animals in Research. [PDF]

J Am Assoc Lab Anim Sci
Clemons DJ, Leland S.
europepmc   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Preventing regrettable substitutions: a proposal for a tiered testing approach for BPA alternatives under the PARC initiative. [PDF]

Front Toxicol
Aiello-Holden K, Bartmann K, Buratti FM, Cabaton NJ, Coppola L, Corsini E, De la Vieja A, Dirven H, Dusinska M, El Yamani N, Franko N, Gostner JM, Grova N, Gütter L, Iglesias-Hernández P, Iulini M, Kodila A, Kohl Y, Lori G, Louro H, Marcon F, Mhaouty-Kodja S, Mposhi A, Naulé L, Palleschi S, Rossi B, Rundén-Pran E, Salani F, Santori N, Silva MJ, Dolenc MS, Testai E, Torres-Ruiz M, Turner J, Viguié C, Zalko D, Tait S.   +36 more
europepmc   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source