Investigation of thePseudomonas aeruginosa ampRgene and its role at the chromosomalampCβ-lactamase promoter [PDF]
The complete sequence of the Pseudomonas aeruginosa beta-lactamase regulator gene ampR has been determined. The amino acid sequence encoded by this gene is compared with other members of the LysR family of transcription factors. The P. aeruginosa ampC promoter was subcloned and gel mobility shift assays were used to demonstrate specific binding of AmpR
J, Lodge, S, Busby, L, Piddock
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Characterisation of CMY-4, an AmpC-type plasmid-mediated β-lactamase in a Tunisian clinical isolate ofProteus mirabilis [PDF]
A strain of Proteus mirabilis resistant to beta-lactams, including cefoxitin, was isolated from the urine of a woman from Tunisia. Its antibiotic susceptibility pattern and that of the Escherichia coli transconjugant suggested the presence of an AmpC-type beta-lactamase. Two bands of beta-lactamase activity (pI 5.4 and 9.2) were detected by isoelectric
C, Verdet +5 more
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Mutations in theampCpromoter ofEscherichia coliisolates resistant to oxyiminocephalosporins without extended spectrum β-lactamase production [PDF]
Escherichia coli strains showing an increased resistance to oxyiminocephalosporins without an extended spectrum beta-lactamase production have been screened for mutations in their ampC beta-lactamase gene promoter. Mutations were found by direct sequencing of seven promoters at positions -42, -32 (box -35), -18, -1, +5, +24 (attenuator), +31 ...
N, Caroff +4 more
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Plasmid typing and genetic context of AmpC β-lactamases in Enterobacteriaceae lacking inducible chromosomal ampC genes: findings from a Spanish hospital 1999–2007 [PDF]
To gain insights into ampC transmission between bacterial strains.We examined the genetic context of 117 acquired ampC genes from 27119 Enterobacteriaceae collected between 1999 and 2007. Plasmid analysis was carried out by PCR-based replicon or relaxase typing, S1-PFGE and Southern hybridization.
Mata, Caterina +7 more
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Expression of ESBL, MBL and AmpC β lactamases by extra intestinal Escherichia coli isolates: correlation with treatment and clinical outcome [PDF]
Objective:We investigated the expression of Extended Spectrum β-Lactamases (ESBLs), AmpC β lactamases and Carbapenemases in extraintestinal pathogenic Escherichia coli (ExPEC) isolates and correlated with treatment and outcome of the patients. Methods:
Arindam Chakraborty +6 more
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Detection of AmpC β-lactamases in gram-negative bacteria
AmpC β-lactamase genes are clinically important because they often confer resistance to most β-lactams other than 4th-generation cephalosporins and carbapenems.
Qian Zhou +5 more
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Multidrug-resistantPseudomonas aeruginosastrains harbouring R-plasmids and AmpC β-lactamases isolated from hospitalised burn patients in a tertiary care hospital of North India [PDF]
The present study was designed to determine resistance rates and patterns in Pseudomonas aeruginosa isolates obtained from hospitalised burn patients in an Indian tertiary care hospital. To that end, we isolated plasmid(s) from the multidrug-resistant isolates, demonstrated the plasmid-mediated resistance by curing and transformation experiments, and ...
M, Shahid, Abida, Malik, , Sheeba
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The presence of extended-spectrum beta-lactamase-producing Escherichia coli in food animals is a public health concern. The aim of this study was to phenotypically detect the extended β-lactamase and AmpC β-lactamase-producing E.
Marinela TIPIȘCĂ +6 more
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Modification of the three-dimensional method for the detection of AmpC β-lactamase in Enterobacter spp. and Escherichia coli [PDF]
Laboratory failure in the detection of Ambler class C (AmpC) β-lactamases in Gram negative bacteria represents a major cause for its spreading and may be associated with low therapeutic outcome.
Sirwa Mustafa mohammed
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: Objectives: Although Sri Lanka belongs to a region with a high prevalence of extended-spectrum β-lactamase (ESBL), AmpC β-lactamase and carbapenemase-producing Enterobacteriaceae, data regarding antimicrobial resistance (AMR) is limited.
Pandithage Dona Vindya Madushika Perera +7 more
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