Results 301 to 310 of about 157,874 (342)
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Assessing Rosa persica genetic diversity using amplified fragment length polymorphisms analysis
Scientia Horticulturae, 2009Abstract The genetic diversity among 128 Iranian Rosa persica (R. persica) accessions in the different populations was analyzed. Amplified fragment length polymorphisms (AFLP) technique was used to produce 171 polymorphic fragments. The number of polymorphic loci ranged from 101 to 147 and the polymorphism information content (PIC) varied from 0.289 ...
T. Basaki +7 more
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Fluorescent Amplified Fragment Length Polymorphism (fAFLP) Analysis of Listeria monocytogenes
2014Fluorescent amplified fragment length polymorphism (fAFLP) is based on the selective PCR amplification of restriction fragments from a digest of total genomic DNA. Genomic DNA extracted from a purified bacterial isolate is completely digested with two endonucleases generating fragments which are ligated to specific double-stranded adaptors. The ligated
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Journal of Microbiological Methods, 2011
Fifty Listeria monocytogenes strains were genotyped by sAFLP and PCR products were separated by agarose gel and automated chip-based microfluidic electrophoresis. A high congruency of results was observed comparing the two techniques, although for some cultures a better separation of sAFLP fragments was achieved with microfluidic system, which proved ...
SAMMARCO, Michela Lucia +4 more
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Fifty Listeria monocytogenes strains were genotyped by sAFLP and PCR products were separated by agarose gel and automated chip-based microfluidic electrophoresis. A high congruency of results was observed comparing the two techniques, although for some cultures a better separation of sAFLP fragments was achieved with microfluidic system, which proved ...
SAMMARCO, Michela Lucia +4 more
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Amplified fragment length polymorphism analysis of the VNTR locus D1S80 in central Spain
International Journal of Legal Medicine, 1993The polymorphism of the D1S80 locus has been analyzed in a population sample of 203 unrelated individuals living in Madrid (central Spain) by PCR and subsequent semi-dry discontinuous polyacrylamide gel electrophoresis (Tris-chloride/Tris-glycine buffer system) followed by silver staining.
A, Alonso +3 more
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Diagnostic Microbiology and Infectious Disease, 2001
Despite major progress in their treatment and prevention, bacterial infections remain a significant cause of morbidity and mortality worldwide. In responding to a disease outbreak, rapid and accurate identification of the bacterial species involved is of paramount importance.
N, Velappan +4 more
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Despite major progress in their treatment and prevention, bacterial infections remain a significant cause of morbidity and mortality worldwide. In responding to a disease outbreak, rapid and accurate identification of the bacterial species involved is of paramount importance.
N, Velappan +4 more
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Beneficial Microbes, 2014
Lactic acid bacteria strains are commonly used for animal and human consumption due to their probiotic properties. One of the major genera used is Lactobacillus, a highly diverse genus comprised of several closely related species. The selection of new strains for probiotic use, especially strains of Lactobacillus, is the focus of several research ...
S H C, Sandes +7 more
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Lactic acid bacteria strains are commonly used for animal and human consumption due to their probiotic properties. One of the major genera used is Lactobacillus, a highly diverse genus comprised of several closely related species. The selection of new strains for probiotic use, especially strains of Lactobacillus, is the focus of several research ...
S H C, Sandes +7 more
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Automated Analysis of Fluorescent Amplified Fragment Length Polymorphisms for DNA Typing
1992We have utilized fluorescently labeled PCR primers to amplify 20 ng of genomic DNA at three different VNTR loci. By using an instrument capable of discriminating the fluorescence emitted from four different dyes, it was possible to analyze the fluorescent PCR products from the three VNTR loci along with an in-lane fluorescently labeled size standard ...
J. Robertson +3 more
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1994
Genetic typing of Variable Number of Tandem Repeat (VNTR) loci is today feasible by the use of fluorescent primers in otherwise typical PCR reactions, and by subsequent laser-detection of amplified products during the electrophoretic run. These facilities, derived from procedures now currently used in automated DNA sequencing, allow for rapid and ...
DOBOSZ, Marina +4 more
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Genetic typing of Variable Number of Tandem Repeat (VNTR) loci is today feasible by the use of fluorescent primers in otherwise typical PCR reactions, and by subsequent laser-detection of amplified products during the electrophoretic run. These facilities, derived from procedures now currently used in automated DNA sequencing, allow for rapid and ...
DOBOSZ, Marina +4 more
openaire +2 more sources