Results 1 to 10 of about 218,617 (259)

Quantitative Polymerase Chain Reaction

open access: yes, 2007
Quantitative PCR (qPCR) has entered widespread use with the increasing availability of real-time PCR. By the incorporation of fluorescent dyes in the reaction mixture, increases in amplification products can be monitored throughout the reaction, enabling measurements to be taken in the exponential phase of the reaction, before the reaction plateau ...
Peirson, S, Butler, J
openaire   +3 more sources

Distinct characteristics on mixed infection of SARS-CoV-2 variants and other respiratory pathogens among patients with acute COVID-19 in central China

open access: yesFrontiers in Cellular and Infection Microbiology
BackgroundReports on mixed infection with different severe acute respiratory syndrome coronavirus 2 variants and other respiratory pathogens in patients with acute coronavirus disease in China remain scarce.
Yiman Geng   +11 more
doaj   +1 more source

Detecting beef meatball contamination with polymerase chain reaction [PDF]

open access: yesBiotika, 2017
The objective of the study was to describe how much rat and swine primer developed from cytochrome b could detect rat and pork in processed beef products sold in North Maluku.
Hoda A., Sapsuha Y., Syafie Y.
doaj  

A simple, rapid and economic method for detecting multidrug-resistant tuberculosis

open access: yesBrazilian Journal of Infectious Diseases, 2013
Objective: To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis. Methods: Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 ...
Xia Wang   +5 more
doaj   +1 more source

DIAGNOSIS OF MALARIA BY REAL-TIME POLYMERASE CHAIN REACTION IN CASES OF NEGATIVE MALARIAL PARASITE ON MICROSCOPY

open access: yesPakistan Armed Forces Medical Journal, 2020
Objective: To determine the frequency of malaria by polymerase chain reaction in malarial parasite negative samples on microscopy. Study Design: Cross-sectional study.
Hina Mushtaq   +5 more
doaj  

Polymerase chain reaction-based gene removal from plasmids

open access: yesData in Brief, 2015
This data article contains supplementary figures and methods to the research article entitled, “Multiplex gene removal by two-step polymerase chain reactions” (Krishnamurthy et al., Anal.
Vishnu Vardhan Krishnamurthy   +4 more
doaj   +1 more source

Design of an Improved Polymerase Chain Reaction (PCR) Assay for Molecular Detection of Streptococcus pneumonia

open access: yesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, 2013
Background and Objectives: Streptococcus pneumoniae accounts for bacterial meningitis and is an important cause of morbidity among children and elderly. Control of this disease depends on rapid detection of the causative bacteria.
Fahimeh Aghamiri   +3 more
doaj   +2 more sources

Development of Polymerase Chain Reaction assays with host-specific internal controls for Chlamydophila abortus

open access: yesVeterinární Medicína, 2015
Chlamydophila abortus (C. abortus) is one of the most important infectious agents causing abortion in ruminants. The bacterium is obligately intracellular, cannot grow on agar, but it needs cell culture or embryonated eggs for growth.
Z. Cantekin   +3 more
doaj   +1 more source

DIAGNOSIS OF CANINE TRYPANOSOMOSIS BY POLYMERASE CHAIN REACTION [PDF]

open access: yesJournal of Veterinary and Animal Sciences, 2013
A clinical case of trypanosomosis in a German Shepherd dog and its diagnosis by polymerase chain reaction(PCR) was described.The dog was presented with clinical signs such as pyrexia, bilateral corneal opacity, pale mucous membranes, emaciation and ...
P.V.Tresamol   +4 more
doaj  

High throughput oscillating-flow structure for polymerase chain reaction

open access: yesAIP Advances
Polymerase chain reaction (PCR) is a revolutionary biomolecule technology that drives the development of bioscience. The current work reports a high throughput oscillating-flow PCR structure.
Ranran Zhou   +7 more
doaj   +1 more source

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