Results 1 to 10 of about 652,487 (129)

Suspending samples over carbon holey films increases heterogeneity of molecular orientations in negative stain electron microscopy

MethodsX, 2019
Negative stain electron microscopy (NSEM) is a simple and cost effective method to analyze a wide variety of specimens, especially proteins. In traditional NSEM, the protein sample is applied to and supported by a continuous carbon film.
Katayoun Mansouri, Robert J. Edwards
doaj   +3 more sources

Evaluation of Pot’s Technique and Sodium Hypochlorite Concentration Technique in Smear Negative Sputum Samples for Increased Sensitivity of Sputum Microscopy [PDF]

Journal of Clinical and Diagnostic Research, 2019
Introduction: In developing countries, diagnosis of pulmonary tuberculosis is primarily based on microscopy owing to its simplicity, less cost and rapidity.
Priya Singhania, Surender Kaur, Navinchandra M Kaore, Shantaram K Gautam, Shilpa N Kaore, Anwari Tayyaba   +5 more
doaj   +2 more sources

Performance of Xpert MTB/RIF and acid-fast bacilli smear microscopy for the diagnosis of pulmonary tuberculosis using bronchoalveolar lavage samples in negative or sputum-scarce adults in Colombia: a retrospective diagnostic accuracy study

BMC Infectious Diseases
Background Limited literature exists on the diagnostic yield of traditional and molecular tuberculosis (TB) tests using bronchoalveolar lavage (BAL) specimens in high burden South American countries. The aim of the study is to determine the value of acid-
Vanessa Sabella-Jiménez, Valentina L. Sabella-Jiménez, Valentina Restrepo-Espinosa, Juanita Eljadue-Flórez, Claredit Valentina Gallardo-Castro, Andrea Alexandra Silvera, Carlos Otero-Herrera, Hugo Andrés Macareno Arroyo, Jorge Acosta-Reyes, Jorge Luis Quintero Barrios   +9 more
doaj   +3 more sources

Rapid Semiquantitative Method for Screening Large Numbers of Virus Samples by Negative Staining Electron Microscopy [PDF]

Applied Microbiology, 1970
A rapid, semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy is presented. Results obtained by this method are compared with results obtained by the pseudoreplica method and a measureddrop method. A figure is presented which represents the limit of detectability for virus by negative staining.
J H, Monroe, P M, Brandt
openaire   +3 more sources

Prevalence of Pfhrp2/3 gene deletions among false negative rapid antigen test results in central India

Journal of Vector Borne Diseases, 2021
Background &objectives: The diagnosis of Plasmodium falciparum malaria is widely dependent on the P. falciparum histidine rich protein 2 (PfHRP2) antigens based rapid diagnostic tests.
Sarita Kumari, Md Zohaib Ahmed, Supriya Sharma, Veena Pande, Anupkumar R Anvikar   +4 more
doaj   +1 more source

Low frequency of Plasmodium falciparum hrp2/3 deletions from symptomatic infections at a primary healthcare facility in Kilifi, Kenya

Frontiers in Epidemiology, 2023
There is a growing concern for malaria control in the Horn of Africa region due to the spread and rise in the frequency of Plasmodium falciparum Histidine-rich Protein (hrp) 2 and 3 deletions.
Dorcas Okanda, Leonard Ndwiga, Victor Osoti, Nicole Achieng, Juliana Wambua, Caroline Ngetsa, Peter Lubell-Doughtie, Anuraj Shankar, Philip Bejon, Philip Bejon, Lynette Isabella Ochola-Oyier   +10 more
doaj   +1 more source

Comparison of Light Emitting Diode (LED) Fluorescent Microscopy with Ziehl-Neelsen Microscopy on Sputum Specimens for Diagnosis of Pulmonary Tuberculosis Keeping Culture as ‘Gold Standard’

Pakistan Armed Forces Medical Journal, 2022
Objective: To compare LED fluorescence microscopy and Ziehl-Neelsen staining in terms of their diagnostic performance in diagnosing pulmonary tuberculosis, taking sputum specimens from patients suspected of pulmonary tuberculosis.
Fatima Tuz Zahra, Muhammad Zeeshan Saleem, Irfan Ali Mirza, Fatima Sana, Ashfaq Hussain, Syed Qamar Zaman   +5 more
doaj   +1 more source

DIAGNOSIS OF MALARIA BY REAL-TIME POLYMERASE CHAIN REACTION IN CASES OF NEGATIVE MALARIAL PARASITE ON MICROSCOPY

Pakistan Armed Forces Medical Journal, 2020
Objective: To determine the frequency of malaria by polymerase chain reaction in malarial parasite negative samples on microscopy. Study Design: Cross-sectional study.
Hina Mushtaq, Mariam Raza, Makki Muhammad, Tarique Arian, Imran Khan, Nighat Afridi   +5 more
doaj   +4 more sources

Comparison of Polymerase Chain Reaction With Fluorescent Microscopy for Detecting Pulmonary Tuberculosis in Smear-Negative Sputum Samples in Quetta, Pakistan

International Journal of Enteric Pathogens, 2021
Background: One of the common clinical manifestations of extra-pulmonary tuberculosis is tuberculous meningitis (TBM). New methods, which are simple and effective, are necessary for early diagnosis of TBM. Objective: This study aimed to assess the use of fluorescence microscopy and polymerase chain reaction (PCR) techniques targeting pncA and IS6110 ...
Muhammad Rizwan, Abdul Samad, Farah Sabeen Bugti, Muhammad Naeem, Safiullah Khan Achakzia, Mahmoud E. Gadalla, Asmaa Ibrahim   +6 more
openaire   +1 more source

No evidence of false-negative Plasmodium falciparum rapid diagnostic results in Monrovia, Liberia

Malaria Journal, 2021
Background Malaria diagnosis in many malaria-endemic countries relies mainly on the use of rapid diagnostic tests (RDTs). The majority of commercial RDTs used in Africa detect the Plasmodium falciparum histidine-rich protein 2 (PfHRP2).
Mandella King, Alexander E. George, Pau Cisteró, Christine K. Tarr-Attia, Beatriz Arregui, Senga Omeonga, Haily Chen, Ana Meyer García-Sípido, Adelaida Sarukhan, Quique Bassat, Dawoh Peter Lansana, Alfredo Mayor   +11 more
doaj   +1 more source