Results 11 to 20 of about 1,424,412 (299)

Microfluidic polymerase chain reaction [PDF]

open access: yes, 2008
We implement microfluidic technology to miniaturize a thermal cycling system for amplifying DNA fragments. By using a microfluidic thermal heat exchanger to cool a Peltier junction, we have demonstrated rapid heating and cooling of small volumes of ...
Gomez, Alvaro   +4 more
core   +1 more source

Efficiency of polymerase chain reaction processes: A stochastic model [PDF]

open access: yes, 2006
A stochastic model for the efficiency of polymerase chain reaction (PCR) processes is presented. The model is based on the assumption that the i’th nucleotide incorporation on the DNA template takes τi seconds, where τi has an exponential distribution ...
Hassibi, Arjang, Sharif, Masoud
core   +1 more source

Development of nested polymerase chain reaction-based diagnosis of duck enteritis virus and detection of DNA polymerase gene from non-descriptive duck breeds of West Bengal, India [PDF]

open access: yesVeterinary World, 2017
Aim: The study was undertaken to detect the clinical signs, postmortem lesions of embryonated duck plague (DP) infected eggs, and histopathological changes of chorioallantoic membrane (CAM) in non-descriptive ducks of West Bengal with special reference ...
Partha Sarathi Mandal   +6 more
doaj   +1 more source

Case Report: Identification of Maternal Low-Level Mosaicism in the Dystrophin Gene by Droplet Digital Polymerase Chain Reaction

open access: yesFrontiers in Genetics, 2021
Germline mosaicism should be suspected when the same de novo mutations are identified in a second pregnancy with asymptomatic parents. Our study aims to find a feasible approach to reveal the existence of germline mosaicism.
Pengzhen Jin   +17 more
doaj   +1 more source

Contribution of neutrophil/lymphocyte ratio to the diagnostic efficiency of computed tomography and polymerase chain reaction in COVID-19 patients

open access: yesSAGE Open Medicine, 2021
Background: 6.5% of the country’s population was diagnosed with COVID-19 disease. Computed tomography scanning and polymerase chain reaction tests are considered reliable methods for the detection of COVID-19.
Serhat Örün, Mustafa Numan Erdem
doaj   +1 more source

On joint maximum-likelihood estimation of PCR efficiency and initial amount of target [PDF]

open access: yes, 2007
We consider the problem of estimating unknown parameters of the real-time polymerase chain reaction (RTPCR) from noisy observations. The joint ML estimator of the RT-PCR efficiency and the initial number of DNA target molecules is derived.
Hassibi, A., Hassibi, B., Vikalo, H.
core   +1 more source

Polymerase Chain Reaction [PDF]

open access: yesSSRN Electronic Journal, 2020
The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. PCR is now a common and often indispensable technique used in medical and biological research labs for a ...
Aakanchha Jain, Richa Jain, Sourabh Jain
  +4 more sources

DNA extraction and polymerase chain reaction

open access: yesJournal of Cytology, 2019
DNA extraction and polymerase chain reaction (PCR) are the basic techniques employed in the molecular laboratory. This short overview covers various physical and chemical methods used for DNA extraction so as to obtain a good-quality DNA in sufficient ...
Nalini Gupta
doaj   +1 more source

Validation of a Polymerase Chain Reaction technique for Kidd blood group genotyping [PDF]

open access: yes, 2015
The Kidd blood group antigens, Jkª and Jkᵇ , are two of the main surface markers which are found on the membrane of red blood cells. The determination of whether a donor or a recipient has the Jkª and/or the Jkᵇ antigens is crucially important to ...
Borg, Joseph J.   +2 more
core   +1 more source

Identifikasi Cendawan Endofit Menggunakan Teknik Polymerase Chain Reaction (Detection of Endophytic Fungi Using Polymerase Chain Reaction Technique)

open access: yesJurnal Fitopatologi Indonesia, 2013
Yellow leaf curl disease, caused by a member of Begomovirus (Geminiviridae), is one of important diseases of chilli pepper in Indonesia. Exploration of endophytic fungi was initiated in order to find biological control agents for an alternative control ...
Tuti Susanti Legiastuti, Tri Aminingsih
doaj   +1 more source

Home - About - Disclaimer - Privacy