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The polymerase chain reaction [PDF]
The Polymerase Chain Reaction (PCR) is a technique for the in vitro synthesis of billions of copies of a specific nucleic acid sequence by performing successive rounds of in vitro nucleic acid replication.
Paulton, M P, Thomas, P C
core +1 more source
Detection of Brucella canis in blood, urine and seminal fluid of a naturally infected dog by PCR
A brucelose canina é uma doença bacteriana, infectocontagiosa que acomete cães de todo o mundo.O diagnóstico laboratorial é baseado em métodos diretos e indiretos e deve ser realizado quando há evidências de sinais clínicos e epidemiológicos.
Fabiana Sperb Volkweis +5 more
doaj +1 more source
Microbiota of Tayohounta, a fermented baobab flavour food of Benin [PDF]
The present work provides data on the microbial composition of Tayohounta, a product of natural fermentation of baobab seed kernels. Samples were collected from 3 different small scale producers from Benin at the end of the fermentation process ...
Chadare, F.J. +5 more
core +2 more sources
Computing by polymerase chain reaction [PDF]
A mathematical notation is introduced to represent, at a symbolic level, different mechanisms of DNA recombination, and a 'PCR lemma' is proven by analytically describing the combinatorial properties of the polymerase chain reaction process. This approach led to the discovery of novel techniques, based on a form of PCR which we called cross pairing PCR
MANCA, Vincenzo, FRANCO, Giuditta
openaire +3 more sources
Background Determining the infectiousness of patients with coronavirus disease 2019 is crucial for patient management. Medical staff usually refer to the results of reverse transcription polymerase chain reaction tests in conjunction with clinical ...
Masafumi Kanamoto +3 more
doaj +1 more source
Semi-quantitative digital analysis of polymerase chain reactionelectrophoresis gel: Potential applications in low-income veterinary laboratories [PDF]
Aim: The interpretation of conventional polymerase chain reaction (PCR) assay results is often limited to either positive or negative (non-detectable). The more robust quantitative PCR (qPCR) method is mostly reserved for quantitation studies and not a ...
John F. Antiabong +2 more
doaj +1 more source
Eradication of Helicobacter pylori Infection Using 7-day PCR-based Tailored Therapy [PDF]
Background/Aims Standard triple therapy (STT; proton pump inhibitor [PPI]+clarithromycin+amoxicillin) used for Helicobacter pylori (H. pylori) eradication has shown low treatment success rates in recent years, which is most likely attributable to ...
Youngwoo Kim +6 more
doaj +1 more source
Laboratory Detection of Novel Corona Virus 2019 Using Polymerase Chain Reaction [PDF]
Since mid-December 2019, several cases of a pneumonia like disease (with symptoms including fever, difficulty in breathing, cough and invasive lesions on both lungs) of unknown cause have emerged in the central Chinese city of Wuhan.
Chudhary, S. A. (Shafiq) +2 more
core
Effectiveness of rotavirus vaccination in prevention of hospital admissions for rotavirus gastroenteritis among young children in Belgium : case-control study [PDF]
Objective : To evaluate the effectiveness of rotavirus vaccination among young children in Belgium. Design : Prospective case-control study. Setting : Random sample of 39 Belgian hospitals, February 2008 to June 2010.
A. Vergison +18 more
core +1 more source
Digital polymerase chain reaction (dPCR) is an advanced molecular biology technique that provides a precise and absolute quantitation of target nucleic acids, providing significant improvements over traditional real-time PCR (qPCR) methods.
Dongsup LEE
doaj +1 more source

