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Chloroplast-specific universal primers and their uses in plant studies [PDF]
Biologia Plantarum, 2011 Universal (consensus) primers are those primers that have the ability to amplify the targeted region of DNA across a broad range of individuals in a certain group of organisms. In plants, such universal primers have been designed to target regions in the
N. Haider
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Ecology and EvolutionConventional dietary assessments are challenging in hematophagous species, particularly in sea lamprey (Petromyzon marinus). However, recent technological developments and molecular approaches have provided an attractive alternative through the use of ...
Conor O'Kane +5 more
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BMC Ecology and EvolutionMonitoring mollusk biodiversity is a great challenge due to their large diversity and broad distribution. Environmental DNA (eDNA) technology is increasingly applied for biodiversity monitoring, but relevant studies on marine mollusks are still limited ...
Xiaojing Shi +3 more
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Universal Primers for Sequencing DNA Restriction Fragments [PDF]
BioTechniques, 1997 Yeeru J. Chen, Manuel S. Valenzuela
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Indonesian Journal of Halal Research, 2022 Adulterating meat products with several species, including non-halal species, is often found in commercial products. This study aims to design and validate the Cytochrome c oxidase subunit I (CO1) primers to detect the non-halal species.
Joni Kusnadi +5 more
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Improved efficiency and robustness in qPCR and multiplex end-point PCR by twisted intercalating nucleic acid modified primers. [PDF]
PLoS ONE, 2012 We introduce quantitative polymerase chain reaction (qPCR) primers and multiplex end-point PCR primers modified by the addition of a single ortho-Twisted Intercalating Nucleic Acid (o-TINA) molecule at the 5'-end.
Uffe Vest Schneider +5 more
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Allele-Specific PCR for PIK3CA Mutation Detection Using Phosphoryl Guanidine Modified Primers
Diagnostics, 2023 Phosphoryl guanidine (PG) is the novel uncharged modification of internucleotide phosphates of oligonucleotides. Incorporating PG modification into PCR primers leads to increased discrimination between wild-type and mutated DNA, providing extraordinary ...
Alexey S. Chubarov +5 more
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ISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations [PDF]
BIO Web of Conferences, 2021 Using the Diamond DNA kit, high quality nuclear DNA was isolated from dry leaves of the endemic species Scutellaria tuvensis. The selection of primers for ISSR analysis of genetic polymorphism of natural populations is described.
Muraseva Dinara S., Guseva Alexandra A.
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Real-time primer design for DNA chips [PDF]
Proceedings International Parallel and Distributed Processing Symposium, 2004 AbstractThe design of PCR or DNA chip experiments is a time‐consuming process where bioinformatics is extensively used. The selection of the primers, which are immobilized on the DNA chip, requires a complex algorithm. Based on several parameters an optimized set of primers is automatically determined for a given gene sequence.This paper describes a ...
Harald Simmler +2 more
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Comparing Microsporidia-targeting primers for environmental DNA sequencing
Parasite, 2023 Metabarcoding is a powerful tool to detect classical, and well-known “long-branch” Microsporidia in environmental samples. Several primer pairs were developed to target these unique microbial parasites, the majority of which remain undetected when using ...
Doliwa Annemie +3 more
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