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Primers for Animal Mitochondrial DNA: The Importance of Species-Specific Primers
1998In this chapter we outline the procedure for designing oligonucleotide primers for the direct-sequencing of in vitro amplified animal mitochondrial DNA (mtDNA). Although this chapter deals with animal mtDNA in particular, the approach is of course applicable to any gene, nuclear or mitochondrial. There are four main ‘steps’:identification of a suitable
Palsboll, Per, ARCTANDER, P
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Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Science, 1988A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus , greatly simplifies the procedure and, by enabling the amplification reaction to be performed at higher temperatures, significantly improves the specificity ...
R K, Saiki +7 more
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Blood, 2015
Abstract RNA-guided Cas9 nucleases based on the prokaryotic CRISPR-Cas systems provide an unprecedented ease in being able to edit the genomes of diverse organisms. As a single unifying factor capable of co-localizing RNA, DNA, and protein, I believe tools and techniques based on Cas9 will grant exquisite control over cellular ...
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Abstract RNA-guided Cas9 nucleases based on the prokaryotic CRISPR-Cas systems provide an unprecedented ease in being able to edit the genomes of diverse organisms. As a single unifying factor capable of co-localizing RNA, DNA, and protein, I believe tools and techniques based on Cas9 will grant exquisite control over cellular ...
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Introduction: DNA Basics—A Primer on DNA
2020To better understand the contents of this book, the lay reader needs to know some basic facts about DNA, and the aim of the following brief introduction is to provide such a necessary background. The reader may also bump in the next chapters into some unusual terms so the Glossary at the end of this book should help him/her to understand the meaning of
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An Environmental DNA Primer for Microbial and Restoration Ecology
Microbial Ecology, 2023Environmental DNA (eDNA) sequencing-DNA collected from the environment from living cells or shed DNA-was first developed for working with microbes and has greatly benefitted microbial ecologists for decades since. These tools have only become increasingly powerful with the advent of metabarcoding and metagenomics.
Michael Tessler +4 more
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Universal COI primers for DNA barcoding amphibians
Molecular Ecology Resources, 2011AbstractDNA barcoding is a proven tool for the rapid and unambiguous identification of species, which is essential for many activities including the vouchering tissue samples in the genome 10K initiative, genealogical reconstructions, forensics and biodiversity surveys, among many other applications.
Jing, Che +7 more
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Effect of Irradiation of the Primer DNA on the Incorporation of Precursors
International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1961SummaryThe rate of enzymatic incorporation of 3H-labelled thymidine into primer DNA was studied after the DNA had been irradiated with doses up to 360 000 roentgens of gamma-rays. Non-irradiated DNA-synthesizing enzyme obtained from regenerating rat-liver was used together with DNA prepared by the method of Kay, Simmons and Dounce (1952) in the ...
C M, WHEELER, S, OKADA
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Ribosomal DNA Probes and Primers
1998Ribosomal DNA (rDNA) is the region of the genome coding for the RNA component of ribosomes. The ubiquity and conservation of rDNA sequences has made these DNA regions important tools for reconstructing phylogenetic relationships. In eukaryotes, rDNA sequences may be found in the nucleus and organelles.
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“Primer” in DNA Polymerase Reactions
1963Publisher Summary This chapter reviews two degrees of primer activation. The first concerns the secondary structure of the DNA molecule, and for the calf thymus and phage-induced E. coli polymerase this type of activation, typically produced by heat, acid, or alkaline denaturation, results in rate increases of one to two orders of magnitude.
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DNA Extraction and Primer Selection
2013Talk regarding pitfalls in DNA extraction and 16S amplicon primer choice when performing community analysis of complex microbial communities. The talk was a part of Workshop 2 "Principles, Potential, and Limitations of Novel Molecular Methods in Water Engineering; from Amplicon Sequencing to -omics Methods" given at the MEWE 2013 conference in Ann ...
Karst, Søren Michael; id_orcid 0000-0002-0233-160X +3 more
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