Results 1 to 10 of about 1,054,678 (119)
Indonesian Journal of Halal Research, 2022 Adulterating meat products with several species, including non-halal species, is often found in commercial products. This study aims to design and validate the Cytochrome c oxidase subunit I (CO1) primers to detect the non-halal species.
Joni Kusnadi +5 more
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Improved efficiency and robustness in qPCR and multiplex end-point PCR by twisted intercalating nucleic acid modified primers. [PDF]
PLoS ONE, 2012 We introduce quantitative polymerase chain reaction (qPCR) primers and multiplex end-point PCR primers modified by the addition of a single ortho-Twisted Intercalating Nucleic Acid (o-TINA) molecule at the 5'-end.
Uffe Vest Schneider +5 more
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Allele-Specific PCR for PIK3CA Mutation Detection Using Phosphoryl Guanidine Modified Primers
Diagnostics, 2023 Phosphoryl guanidine (PG) is the novel uncharged modification of internucleotide phosphates of oligonucleotides. Incorporating PG modification into PCR primers leads to increased discrimination between wild-type and mutated DNA, providing extraordinary ...
Alexey S. Chubarov +5 more
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ISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations [PDF]
BIO Web of Conferences, 2021 Using the Diamond DNA kit, high quality nuclear DNA was isolated from dry leaves of the endemic species Scutellaria tuvensis. The selection of primers for ISSR analysis of genetic polymorphism of natural populations is described.
Muraseva Dinara S., Guseva Alexandra A.
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Comparing Microsporidia-targeting primers for environmental DNA sequencing
Parasite, 2023 Metabarcoding is a powerful tool to detect classical, and well-known “long-branch” Microsporidia in environmental samples. Several primer pairs were developed to target these unique microbial parasites, the majority of which remain undetected when using ...
Doliwa Annemie +3 more
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Zanco Journal of Pure and Applied Sciences, 2020 The aim of present study to determine DNA polymorphism, between and within two different color (white) and (black) guinea fowl lines. Blood samples were collected about 3 ml of 30 white guinea fowl and 30 black guinea fowl, in six different local farms.
Hurea S. Abdulrazaq
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Computational and Structural Biotechnology Journal, 2023 Polymerase chain reaction (PCR) is a powerful molecular biology assay for gene detection and quantification. Conventional DNA primers for PCR often suffer from poor sensitivity in specific gene detection.
Yi-Chen Tsai +2 more
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Optimization of ISSR-PCR reaction system and selection of primers in Bryum argenteum
上海师范大学学报. 自然科学版, 2017 In order to determine optimum ISSR-PCR reaction system for moss Bryum argenteum,the concentrations of template DNA primers,dNTPs,Mg2+ and Taq DNA polymerase were optimized in four levels by PCR orthogonal experimental method. The appropriate primers were
Ma Xiaoying +3 more
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Journal of Food Quality and Hazards Control, 2021 Background: Species identification in commercially processed food and feed products is one of the important issues. This study was conducted to develop a genetic method for the detection of pig and cattle species in processed food and feed products using
D.C. Roy +7 more
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Comparison of different primes for PCR-based diagnosis of cutaneous leishmaniasis
Brazilian Journal of Infectious Diseases, 2011 Objective: The objective of this study was to analyze different primers that are commonly used in epidemiological studies for the detection of Leishmania DNA by PCR, and to compare them to the conventional direct parasite search for American cutaneous ...
Diego Molina de Oliveira, M.D. +3 more
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