Results 21 to 30 of about 2,987,670 (314)
Scientific Reports, 2023 16S rRNA amplicon sequencing or, more recently, metatranscriptomic analysis are currently the only preferred methods for microbial profiling of samples containing a predominant ratio of human to bacterial DNA. However, due to the off-target amplification
Tereza Deissová +7 more
doaj +1 more source
Molecular basis for the initiation of DNA primer synthesis
Nature, 2022 During the initiation of DNA replication, oligonucleotide primers are synthesized de novo by primases and are subsequently extended by replicative polymerases to complete genome duplication. The primase-polymerase (Prim-Pol) superfamily is a diverse grouping of primases, which includes replicative primases and CRISPR-associated primase-polymerases ...
Arthur W. H. Li +8 more
openaire +2 more sources
Accidental Amplification of the Avian Influenza N4 Gene Segment by N1-Specific Primers
مجلة الانبار للعلوم البيطريةBackground: Avian influenza is a worldwide animal and public health risk. With increasing bird-to-human transmission, it has become a significant infectious agent.
Khlef, Evan Latef
doaj +1 more source
Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers
BMC Biotechnology, 2011 Background MicroRNAs are important regulators of gene expression at the post-transcriptional level and play an important role in many biological processes.
Busk Peter K +2 more
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Primers used to amplify 16S DNA.
, 2014 Equimolar amounts of all 6 forward primers and all 6 reverse primers were pooled and used in PCR. Forward primers span nt 926–950; reverse primers span nt 1107–1129 (numbering relative to E. coli 16S DNA).Primers used to amplify 16S DNA.
Elizabeth E. Rogers (110615) +1 more
core +1 more source
F1000Research, 2021 The prevalence of Herpes simplex virus 2 (HSV2) is increasing at an alarming rate in the world. Most of the HSV2 cases are not diagnosed properly, although a range of molecular and serological diagnoses exist.
Modhusudon Shaha +2 more
doaj +1 more source
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
DNA primers used in this study.
, 2015 DNA primers used in this study.
Corrella S. Detweiler (366707) +3 more
core +2 more sources