Results 21 to 30 of about 1,480,527 (305)
Using long ssDNA polynucleotides to amplify STRs loci in degraded DNA samples. [PDF]
PLoS ONE, 2017 Obtaining informative short tandem repeat (STR) profiles from degraded DNA samples is a challenging task usually undermined by locus or allele dropouts and peak-high imbalances observed in capillary electrophoresis (CE) electropherograms, especially for ...
Martín E Mautner +5 more
doaj +1 more source
Intra- and interspecific polymorphisms ofLeishmania donovani andL. tropica minicircle DNA [PDF]
, 1994 A pair of degenerate polymerase chain reaction (PCR) primers (LEI-1, TCG GAT CC[C,T] [G,C]TG GGT AGG GGC GT; LEI-2, ACG GAT CC[G,C] [G,C][A,C]C TAT [A,T]TT ACA CC) defining a 0.15-kb segment ofLeishmania minicircle DNA was constructed.
AJ Magill +16 more
core +1 more source
Indonesian Journal of Human Nutrition, 2021 Composition adulteration in a product is still a serious problem. For that we need a DNA-based test method that is fast, specific and simple. In this study, a rapid test technique with the loop-mediated isothermal amplification method was applied to ...
Rahma Micho Widyanto +6 more
doaj +1 more source
Identification and Validation of EST-Derived Molecular Markers, TRAP and VNTRs, for Banana Research [PDF]
, 2011 The advent of high-throughput sequencing technology has generated abundant information on DNA sequences for the genomes of many plant species. Expressed Sequence Tags (ESTs), which are unique DNA sequences derived from a cDNA library and therefore ...
Ferreira, C.F. +6 more
core +2 more sources
Scientific Reports, 2023 16S rRNA amplicon sequencing or, more recently, metatranscriptomic analysis are currently the only preferred methods for microbial profiling of samples containing a predominant ratio of human to bacterial DNA. However, due to the off-target amplification
Tereza Deissová +7 more
doaj +1 more source
Template-dependent multiple displacement amplification for profiling human circulating RNA [PDF]
, 2017 Multiple displacement amplification (MDA) is widely used in whole-genome/transcriptome amplification. However, template-independent amplification (TIA) in MDA is a commonly observed phenomenon, particularly when using high concentrations of random ...
Crosby, Seth D +6 more
core +2 more sources
High-throughput sequencing of 16S rRNA gene amplicons : effects of extraction procedure, primer length and annealing temperature [PDF]
, 2012 The analysis of 16S-rDNA sequences to assess the bacterial community composition of a sample is a widely used technique that has increased with the advent of high throughput sequencing.
Cogan, Tristan +4 more
core +3 more sources
Accidental Amplification of the Avian Influenza N4 Gene Segment by N1-Specific Primers
مجلة الانبار للعلوم البيطريةBackground: Avian influenza is a worldwide animal and public health risk. With increasing bird-to-human transmission, it has become a significant infectious agent.
Khlef, Evan Latef
doaj +1 more source
Eliminating mitochondrial DNA competition for nuclear DNA primers. [PDF]
Genome Research, 1993 Mitochondrial DNA (mtDNA) sequences were synthesized with nuclear DNA (nucDNA) sequence-tagged site (STS) primers by mismatch priming in three independent studies of the human nuclear genome. Mismatch primer binding sites on the mtDNA were identified with from 6- to 10-bp identity at the 3' ends of the primers.
S, Zullo +8 more
openaire +2 more sources
Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers
BMC Biotechnology, 2011 Background MicroRNAs are important regulators of gene expression at the post-transcriptional level and play an important role in many biological processes.
Busk Peter K +2 more
doaj +1 more source