Results 21 to 30 of about 2,585,724 (312)

Molecular characterization of Cryptosporidium isolates from humans and other animals using random amplified polymorphic DNA analysis [PDF]

, 1995
Genetic variation in 25 Cryptosporidium isolates was analyzed using the random amplified polymorphic DNA (RAPD) technique. Simple reproducible polymorphisms were generated (using five primers) from Cryptosporidium DNA that was free of contaminating ...
O'Donoghue, P., Meloni, BP, Meloni, B.P., Morgan, UM, Thompson, R.C.A., Morgan, U.M., R. C. Andrew Thompson, Peter O'Donoghue, Constantine, CC, Philip A. O'Brien, Clare C. Constantine, Constantine, C.C., Odonoghue, P, Obrien, PA, O'Brien, P.A., Bruno P. Meloni, Thompson, Rca, Una M. Morgan   +17 more
core   +1 more source

Molecular effects of site-specific phosphate-methylated primer on the structure and motions of Taq DNA polymerase

Computational and Structural Biotechnology Journal, 2023
Polymerase chain reaction (PCR) is a powerful molecular biology assay for gene detection and quantification. Conventional DNA primers for PCR often suffer from poor sensitivity in specific gene detection.
Yi-Chen Tsai, Wen-Yih Chen, Chi-cheng Chiu   +2 more
doaj   +1 more source

DNA-based faecal dietary analysis: A comparison of qPCR and high throughput sequencing approaches [PDF]

, 2011
The genetic analysis of faecal material represents a relatively non-invasive way to study animal diet and has been widely adopted in ecological research.
Michael Bunce, Haile, James, Roberto A Barrero, Jayne Houston, White Nicole E., Haile James, Belinda L. Cannell, Rebecca Oliver, Oliver, R., Dáithí C. Murray, Bellgard, M.I., Houston Jayne, Murray, D.C., Dáithí C Murray, Belinda L Cannell, White, N.E., Bellgard Matthew I., Barrero, R., Oliver Rebecca, Matthew I Bellgard, Murray Dáithí C., Cannell, B.L., Barrero Roberto A., Haile, J., Matthew I. Bellgard, White, Nicole, Houston, J., Bellgard, M., Murray, D., James Haile, Cannell, B., Barrero, R.A., Nicole E White, Bunce, M., Bunce Michael, Cannell Belinda L., Nicole E. White, Roberto A. Barrero, Bunce, Michael   +38 more
core   +1 more source

High-throughput sequencing of 16S rRNA gene amplicons : effects of extraction procedure, primer length and annealing temperature [PDF]

, 2012
The analysis of 16S-rDNA sequences to assess the bacterial community composition of a sample is a widely used technique that has increased with the advent of high throughput sequencing.
Sergeant Martin J., Penn, Charles W, Cogan Tristan, Pallen, Mark J., Charles W Penn, Chrystala Constantinidou (161352), Charles W. Penn (161371), Sergeant, MJ, Cogan, Tristan, Tristan Cogan (161360), Constantinidou Chrystala, Constantinido, C, Mark J Pallen, Tristan Cogan, Martin J. Sergeant (161344), Constantinidou, Chrystala, Sergeant, Martin J, Penn Charles W., Pallen, Mark J, Sergeant, Martin J., Chrystala Constantinidou, Mark J. Pallen (8904), Penn, CW, Pallen, MJ, Penn, Charles W., Pallen Mark J., Martin J Sergeant, Cogan, TA   +27 more
core   +2 more sources

Optimization of ISSR-PCR reaction system and selection of primers in Bryum argenteum

上海师范大学学报. 自然科学版, 2017
In order to determine optimum ISSR-PCR reaction system for moss Bryum argenteum,the concentrations of template DNA primers,dNTPs,Mg2+ and Taq DNA polymerase were optimized in four levels by PCR orthogonal experimental method. The appropriate primers were
Ma Xiaoying, Wei Wei, Zang Cheng, Yu Jing   +3 more
doaj   +1 more source

Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]

, 1992
Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
Dunham, Ian, Cremer, Thomas, Terry Featherstone, Christoph Lengauer, Featherstone, Terry, Lan Dunham, Lengauer, Christoph, Thomas Cremer   +7 more
core   +1 more source

Comparison of different primes for PCR-based diagnosis of cutaneous leishmaniasis

Brazilian Journal of Infectious Diseases, 2011
Objective: The objective of this study was to analyze different primers that are commonly used in epidemiological studies for the detection of Leishmania DNA by PCR, and to compare them to the conventional direct parasite search for American cutaneous ...
Diego Molina de Oliveira, M.D., Maria Valdrinez Campana Lonardoni, M.D.Ph.D., Ueslei Teodoro, Thais Gomes Verzignassi Silveira   +3 more
doaj   +1 more source

MCMC-ODPR : primer design optimization using Markov Chain Monte Carlo sampling [PDF]

, 2012
Background Next generation sequencing technologies often require numerous primer designs that require good target coverage that can be financially costly.
Kitchen, James, Kitchen James L, Allaby Robin G, Moore, Jonathan D., Palmer Sarah A, Allaby, Robin G., Moore Jonathan D, Palmer, Sarah A.   +7 more
core   +1 more source

Tracing the Pig and Cattle Origin in Processed Food and Feed Products Targeting Mitochondrial 12S rRNA Gene

Journal of Food Quality and Hazards Control, 2021
Background: Species identification in commercially processed food and feed products is one of the important issues. This study was conducted to develop a genetic method for the detection of pig and cattle species in processed food and feed products using
D.C. Roy, S. Akhter, A.K. Sarker, M.M.K. Hossain, C. Lyzu, L.C. Mohanta, D. Islam, M.A.A. Khan   +7 more
doaj  

Intra- and interspecific polymorphisms ofLeishmania donovani andL. tropica minicircle DNA [PDF]

, 1994
A pair of degenerate polymerase chain reaction (PCR) primers (LEI-1, TCG GAT CC[C,T] [G,C]TG GGT AGG GGC GT; LEI-2, ACG GAT CC[G,C] [G,C][A,C]C TAT [A,T]TT ACA CC) defining a 0.15-kb segment ofLeishmania minicircle DNA was constructed.
Dobner, P., Rinder, H., Löscher, Thomas
core   +1 more source