Results 21 to 30 of about 1,066,757 (310)
Journal of Food Quality and Hazards Control, 2021 Background: Species identification in commercially processed food and feed products is one of the important issues. This study was conducted to develop a genetic method for the detection of pig and cattle species in processed food and feed products using
D.C. Roy +7 more
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Intra- and interspecific polymorphisms ofLeishmania donovani andL. tropica minicircle DNA [PDF]
, 1994 A pair of degenerate polymerase chain reaction (PCR) primers (LEI-1, TCG GAT CC[C,T] [G,C]TG GGT AGG GGC GT; LEI-2, ACG GAT CC[G,C] [G,C][A,C]C TAT [A,T]TT ACA CC) defining a 0.15-kb segment ofLeishmania minicircle DNA was constructed.
Dobner, P., Rinder, H., Löscher, Thomas
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Using long ssDNA polynucleotides to amplify STRs loci in degraded DNA samples. [PDF]
PLoS ONE, 2017 Obtaining informative short tandem repeat (STR) profiles from degraded DNA samples is a challenging task usually undermined by locus or allele dropouts and peak-high imbalances observed in capillary electrophoresis (CE) electropherograms, especially for ...
Martín E Mautner +5 more
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Molecular basis for the initiation of DNA primer synthesis
Nature, 2022 During the initiation of DNA replication, oligonucleotide primers are synthesized de novo by primases and are subsequently extended by replicative polymerases to complete genome duplication. The primase-polymerase (Prim-Pol) superfamily is a diverse grouping of primases, which includes replicative primases and CRISPR-associated primase-polymerases ...
Arthur W. H. Li +8 more
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Direct amplification and differentiation of pathogenic and nonpathogenic Entamoeba histolytica DNA from stool specimens [PDF]
, 1994 Discrimination of pathogenic and nonpathogenic Entamoeba histolytica is of great clinical importance. A simple and rapid DNA extraction method that can be used directly with stool specimens was developed without the need for prior cultivation of the ...
Katzwinkel-Wladarsch, S. +5 more
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Environmental Status Assessment Using DNA Metabarcoding: Towards a Genetics Based Marine Biotic Index (gAMBI) [PDF]
, 2014 Aylagas, E., Á. Borja, N. Rodríguez-Ezpeleta, 2014. Environmental Status Assessment Using DNA Metabarcoding: Towards a Genetics Based Marine Biotic Index (gAMBI). PLoS ONE, 9(3): e90529.
Rodríguez-Ezpeleta, Naiara +5 more
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Indonesian Journal of Human Nutrition, 2021 Composition adulteration in a product is still a serious problem. For that we need a DNA-based test method that is fast, specific and simple. In this study, a rapid test technique with the loop-mediated isothermal amplification method was applied to ...
Rahma Micho Widyanto +6 more
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Trypanosoma evansi: Genetic variability detected using amplified restriction fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) analysis of Kenyan isolates [PDF]
, 2006 We compared two methods to generate polymorphic markers to investigate the population genetics of Trypanosoma evansi; random amplified polymorphic DNA (RAPD) and amplified restriction fragment length polymorphism (AFLP) analyses.
Ndung’u, K. +6 more
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Scientific Reports, 2023 16S rRNA amplicon sequencing or, more recently, metatranscriptomic analysis are currently the only preferred methods for microbial profiling of samples containing a predominant ratio of human to bacterial DNA. However, due to the off-target amplification
Tereza Deissová +7 more
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Primer initiation and extension by T7 DNA primase [PDF]
The EMBO Journal, 2006 T7 DNA primase is composed of a catalytic RNA polymerase domain (RPD) and a zinc-binding domain (ZBD) connected by an unstructured linker. The two domains are required to initiate the synthesis of the diribonucleotide pppAC and its extension into a functional primer pppACCC (de novo synthesis), as well as for the extension of exogenous AC ...
Udi, Qimron +3 more
openaire +2 more sources