Results 41 to 50 of about 1,066,757 (310)

GST-PRIME: a genome-wide primer design software for the generation of gene sequence tags [PDF]

, 2001
The availability of sequenced genomes has generated a need for experimental approaches that allow the simultaneous analysis of large, or even complete, sets of genes.
Varotto, Claudio, Leister, Dario, Salamini, Francesco, Richly, Erik   +3 more
core   +1 more source

STUDI PERBANDINGAN PEMAKAIAN PRIMER HBS1 DAN HBS2 DENGAN PRIMER P7 DAN P8 DALAM DETEKSI DNA VIRUS HEPATITIS B PADA PENDONOR DARAH

Florea: Jurnal Biologi dan Pembelajarannya, 2020
Examination of the hepatitis B virus as a disease of hepatitis serologically has been carried out a lot, but this serological examination can experience problems if there is a low window period, so even though a negative test result is still possible in ...
Supiana Dian Nurtjahyani, Mohammad Amin, Retno Handajan   +2 more
doaj   +1 more source

PCR primers for metazoan nuclear 18S and 28S ribosomal DNA sequences. [PDF]

PLoS ONE, 2012
BACKGROUND: Metagenetic analyses, which amplify and sequence target marker DNA regions from environmental samples, are increasingly employed to assess the biodiversity of communities of small organisms. Using this approach, our understanding of microbial
Ryuji J Machida, Nancy Knowlton
doaj   +1 more source

Detection of herpes simplex virus 2: a SYBR-Green-based real-time PCR assay [version 2; peer review: 2 approved]

F1000Research, 2021
The prevalence of Herpes simplex virus 2 (HSV2) is increasing at an alarming rate in the world. Most of the HSV2 cases are not diagnosed properly, although a range of molecular and serological diagnoses exist.
Modhusudon Shaha, Mohammad Ariful Islam, Bithi Roy   +2 more
doaj   +1 more source

Clinical, Histopathological, and Molecular Characterization of Pediatric MN1::ZNF341‐Associated Cancer

Pediatric Blood &Cancer, EarlyView.
ABSTRACT A lethal round‐cell malignancy with an MN1::ZNF341 fusion has recently been reported in three infants. Here, we describe four further tumors, three in newborns (including monozygotic twins), and one in an adolescent. Detailed clinical, radiological, and histopathological data differentiate these tumors from their main mimics, neuroblastoma and
Thomas R. W. Oliver, Dyanne Rampling, Barbara Walkowiak, Nathaniel D. Anderson, Manas Dave, Henry Lee‐Six, Sadie Baskind, Pooja Balasubramanian, Glenn Anderson, Conor Parks, Toochi Ogbonnah, Emilia R. Robertson, Simon Hannam, Rohan Verma, John C. Achermann, Liina Palm, Neil Sebire, Thomas Jacques, Tom Watson, Carryl Dryden, Karin Straathof, Mette Jorgensen, Olga Slater, Sam Behjati, Sarita Depani, J. Ciaran Hutchinson   +25 more
wiley   +1 more source

DNA extraction from formalin-fixed tissue: new light from the Deep-Sea [PDF]

, 2010
DNA samples were extracted from ethanol and formalin-fixed decapod crustacean tissue using a new method based on Tetramethylsilane (TMS)-Chelex. It is shown that neither an indigestible matrix of cross-linked protein nor soluble PCR inhibitors impede PCR
Thatje, S., Johnston, J., Johnston, David, Mackenzie-Dodds, Jackie, Clark, P., Hall, S., Palero, F., Mackenzie-Dodds, J., Hall, Sally, Clark, Paul, Palero, Ferran, Mackenzie Dodds, Jackie, Clark, Paul F., Palero, Ferran ; https://orcid.org/, Thatje, Sven   +14 more
core   +2 more sources

Comparison of different primes for PCR-based diagnosis of cutaneous leishmaniasis

Brazilian Journal of Infectious Diseases
OBJECTIVE: The objective of this study was to analyze different primers that are commonly used in epidemiological studies for the detection of Leishmania DNA by PCR, and to compare them to the conventional direct parasite search for American cutaneous ...
Diego Molina de Oliveira, Maria Valdrinez Campana Lonardoni, Ueslei Teodoro, Thais Gomes Verzignassi Silveira   +3 more
doaj   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Primers, probes, DNA oligo templates. [PDF]

, 2019
Primers, probes, DNA oligo templates.
Marcello Marelli (759717), Andrew Garcia (2268778), Christine Kiefer (675617), Susan Wilson (134263), Gargi Roy (4386271)   +4 more
core   +1 more source

Blocking primers to enhance PCR amplification of rare sequences in mixed samples – a case study on prey DNA in Antarctic krill stomachs

Frontiers in Zoology, 2008
Background Identification of DNA sequence diversity is a powerful means for assessing the species present in environmental samples. The most common molecular strategies for estimating taxonomic composition depend upon PCR with universal primers that ...
Jarman Simon N, Vestheim Hege
doaj   +1 more source