Using long ssDNA polynucleotides to amplify STRs loci in degraded DNA samples. [PDF]
PLoS ONE, 2017 Obtaining informative short tandem repeat (STR) profiles from degraded DNA samples is a challenging task usually undermined by locus or allele dropouts and peak-high imbalances observed in capillary electrophoresis (CE) electropherograms, especially for ...
Martín E Mautner +5 more
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DNA Probes and Primers in Dental Practice [PDF]
Clinical Infectious Diseases, 2002 In clinical microbiology, molecular genetic techniques are increasingly being used to detect and/or differentiate uncultivable, anaerobic, or fastidious microorganisms. During the past decade, DNA probe hybridization and in vitro amplification by polymerase chain reaction have also been introduced to detect oral pathogens.
openaire +2 more sources
Indonesian Journal of Human Nutrition, 2021 Composition adulteration in a product is still a serious problem. For that we need a DNA-based test method that is fast, specific and simple. In this study, a rapid test technique with the loop-mediated isothermal amplification method was applied to ...
Rahma Micho Widyanto +6 more
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Environmental Status Assessment Using DNA Metabarcoding: Towards a Genetics Based Marine Biotic Index (gAMBI) [PDF]
, 2014 Aylagas, E., Á. Borja, N. Rodríguez-Ezpeleta, 2014. Environmental Status Assessment Using DNA Metabarcoding: Towards a Genetics Based Marine Biotic Index (gAMBI). PLoS ONE, 9(3): e90529.
Rodríguez-Ezpeleta, Naiara +5 more
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Scientific Reports, 2023 16S rRNA amplicon sequencing or, more recently, metatranscriptomic analysis are currently the only preferred methods for microbial profiling of samples containing a predominant ratio of human to bacterial DNA. However, due to the off-target amplification
Tereza Deissová +7 more
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Trypanosoma evansi: Genetic variability detected using amplified restriction fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) analysis of Kenyan isolates [PDF]
, 2006 We compared two methods to generate polymorphic markers to investigate the population genetics of Trypanosoma evansi; random amplified polymorphic DNA (RAPD) and amplified restriction fragment length polymorphism (AFLP) analyses.
Ndung’u, K. +6 more
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Primer1: Primer design Web service for Tetra-Primer ARMS-PCR [PDF]
, 2012 Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of ...
Ke, Xiayi, Collins, Andrew
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Distribution of TT virus (TTV), TTV-like minivirus, and related viruses in humans and nonhuman primates [PDF]
, 2003 TT virus (TTV) and TTV-like minivirus (TLMV) are small DNA viruses with single-stranded, closed circular, antisense genomes infecting man. Despite their extreme sequence heterogeneity (>50%), a highly conserved region in the untranslated region (UTR ...
Thom, K +4 more
core +1 more source
Accidental Amplification of the Avian Influenza N4 Gene Segment by N1-Specific Primers
مجلة الانبار للعلوم البيطريةBackground: Avian influenza is a worldwide animal and public health risk. With increasing bird-to-human transmission, it has become a significant infectious agent.
Khlef, Evan Latef
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Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers
BMC Biotechnology, 2011 Background MicroRNAs are important regulators of gene expression at the post-transcriptional level and play an important role in many biological processes.
Busk Peter K +2 more
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