Results 31 to 40 of about 2,987,670 (314)

PCR primers for metazoan nuclear 18S and 28S ribosomal DNA sequences. [PDF]

open access: yesPLoS ONE, 2012
BACKGROUND: Metagenetic analyses, which amplify and sequence target marker DNA regions from environmental samples, are increasingly employed to assess the biodiversity of communities of small organisms. Using this approach, our understanding of microbial
Ryuji J Machida, Nancy Knowlton
doaj   +1 more source

Primer initiation and extension by T7 DNA primase [PDF]

open access: yesThe EMBO Journal, 2006
T7 DNA primase is composed of a catalytic RNA polymerase domain (RPD) and a zinc-binding domain (ZBD) connected by an unstructured linker. The two domains are required to initiate the synthesis of the diribonucleotide pppAC and its extension into a functional primer pppACCC (de novo synthesis), as well as for the extension of exogenous AC ...
Udi, Qimron   +3 more
openaire   +2 more sources

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

open access: yesFEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens   +8 more
wiley   +1 more source

Primers, probes, DNA oligo templates.

open access: yes, 2019
Primers, probes, DNA oligo templates.
Marcello Marelli (759717)   +4 more
core   +1 more source

STUDI PERBANDINGAN PEMAKAIAN PRIMER HBS1 DAN HBS2 DENGAN PRIMER P7 DAN P8 DALAM DETEKSI DNA VIRUS HEPATITIS B PADA PENDONOR DARAH

open access: yesFlorea: Jurnal Biologi dan Pembelajarannya, 2020
Examination of the hepatitis B virus as a disease of hepatitis serologically has been carried out a lot, but this serological examination can experience problems if there is a low window period, so even though a negative test result is still possible in ...
Supiana Dian Nurtjahyani   +2 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

open access: yesFEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler   +2 more
wiley   +1 more source

Primers Used for DNA Sequence Analysis.

open access: yes, 2015
Note. Primers were HPLC purified, 50 nmoles from InvitrogenPrimers Used for DNA Sequence Analysis.
Erin Stokes (696333)   +4 more
core   +1 more source

Comparison of different primes for PCR-based diagnosis of cutaneous leishmaniasis

open access: yesBrazilian Journal of Infectious Diseases
OBJECTIVE: The objective of this study was to analyze different primers that are commonly used in epidemiological studies for the detection of Leishmania DNA by PCR, and to compare them to the conventional direct parasite search for American cutaneous ...
Diego Molina de Oliveira   +3 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

open access: yesFEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj   +3 more
wiley   +1 more source

Primers and probes targeting DNA of diarrheagenic agents.

open access: yes, 2022
Primers and probes targeting DNA of diarrheagenic agents.
François-Xavier Weill (157212)   +12 more
core   +1 more source

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