Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]
, 1992 Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
A Gnirke +33 more
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DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity analysis in Citrus [PDF]
, 1995 We used three short repetitive nucleotide sequences [(GTG)5, (TAC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted Citrus DNA or as single primers in polymerase chain reaction amplification experiments with total genomic ...
Asif, M +7 more
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Optimization of ISSR-PCR reaction system and selection of primers in Bryum argenteum
上海师范大学学报. 自然科学版, 2017 In order to determine optimum ISSR-PCR reaction system for moss Bryum argenteum,the concentrations of template DNA primers,dNTPs,Mg2+ and Taq DNA polymerase were optimized in four levels by PCR orthogonal experimental method. The appropriate primers were
Ma Xiaoying +3 more
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16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform [PDF]
, 2016 peer-reviewedBackground Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the
Claesson, Marcus J. +4 more
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An efficient method for DNA extraction from Cladosporioid fungi [PDF]
, 2010 We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Abd-Elsalam, K.A. +3 more
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Comparison of different primes for PCR-based diagnosis of cutaneous leishmaniasis
Brazilian Journal of Infectious Diseases, 2011 Objective: The objective of this study was to analyze different primers that are commonly used in epidemiological studies for the detection of Leishmania DNA by PCR, and to compare them to the conventional direct parasite search for American cutaneous ...
Diego Molina de Oliveira, M.D. +3 more
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PlantID – DNA-based identification of multiple medicinal plants in complex mixtures [PDF]
, 2012 Background An efficient method for the identification of medicinal plant products is now a priority as the global demand increases. This study aims to develop a DNA-based method for the identification and authentication of plant species that can be ...
Bremner, Paul +7 more
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Journal of Food Quality and Hazards Control, 2021 Background: Species identification in commercially processed food and feed products is one of the important issues. This study was conducted to develop a genetic method for the detection of pig and cattle species in processed food and feed products using
D.C. Roy +7 more
doaj
Chloroplast-specific universal primers and their uses in plant studies
Biologia Plantarum, 2011 Universal (consensus) primers are those primers that have the ability to amplify the targeted region of DNA across a broad range of individuals in a certain group of organisms. In plants, such universal primers have been designed to target regions in the
N. Haider
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Ten polymorphic microsatellite primers in the tropical tree caimito, Chrysophyllum cainito (Sapotaceae). [PDF]
, 2014 UnlabelledPremise of the studyWe developed microsatellite primers for the tropical tree Chrysophyllum cainito (Sapotaceae) to determine the native range of the species, investigate the origin of cultivated populations, and examine the partitioning of ...
Parker, Ingrid M +2 more
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