Chloroplast-specific universal primers and their uses in plant studies
Biologia Plantarum, 2011 Universal (consensus) primers are those primers that have the ability to amplify the targeted region of DNA across a broad range of individuals in a certain group of organisms. In plants, such universal primers have been designed to target regions in the
N. Haider
doaj +2 more sources
Universal Primers for Sequencing DNA Restriction Fragments [PDF]
BioTechniques, 1997 Yeeru J. Chen, Manuel S. Valenzuela
doaj +3 more sources
Molecular characterization of Cryptosporidium isolates from humans and other animals using random amplified polymorphic DNA analysis [PDF]
, 1995 Genetic variation in 25 Cryptosporidium isolates was analyzed using the random amplified polymorphic DNA (RAPD) technique. Simple reproducible polymorphisms were generated (using five primers) from Cryptosporidium DNA that was free of contaminating ...
O'Donoghue, P. +17 more
core +1 more source
Real-time primer design for DNA chips [PDF]
Proceedings International Parallel and Distributed Processing Symposium, 2004 AbstractThe design of PCR or DNA chip experiments is a time‐consuming process where bioinformatics is extensively used. The selection of the primers, which are immobilized on the DNA chip, requires a complex algorithm. Based on several parameters an optimized set of primers is automatically determined for a given gene sequence.This paper describes a ...
Harald Simmler +2 more
openaire +2 more sources
DNA-based faecal dietary analysis: A comparison of qPCR and high throughput sequencing approaches [PDF]
, 2011 The genetic analysis of faecal material represents a relatively non-invasive way to study animal diet and has been widely adopted in ecological research.
Michael Bunce +38 more
core +1 more source
High-throughput sequencing of 16S rRNA gene amplicons : effects of extraction procedure, primer length and annealing temperature [PDF]
, 2012 The analysis of 16S-rDNA sequences to assess the bacterial community composition of a sample is a widely used technique that has increased with the advent of high throughput sequencing.
Sergeant Martin J. +27 more
core +2 more sources
Using long ssDNA polynucleotides to amplify STRs loci in degraded DNA samples. [PDF]
PLoS ONE, 2017 Obtaining informative short tandem repeat (STR) profiles from degraded DNA samples is a challenging task usually undermined by locus or allele dropouts and peak-high imbalances observed in capillary electrophoresis (CE) electropherograms, especially for ...
Martín E Mautner +5 more
doaj +1 more source
DNA Probes and Primers in Dental Practice [PDF]
Clinical Infectious Diseases, 2002 In clinical microbiology, molecular genetic techniques are increasingly being used to detect and/or differentiate uncultivable, anaerobic, or fastidious microorganisms. During the past decade, DNA probe hybridization and in vitro amplification by polymerase chain reaction have also been introduced to detect oral pathogens.
openaire +2 more sources
Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]
, 1992 Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
Dunham, Ian +7 more
core +1 more source
Indonesian Journal of Human Nutrition, 2021 Composition adulteration in a product is still a serious problem. For that we need a DNA-based test method that is fast, specific and simple. In this study, a rapid test technique with the loop-mediated isothermal amplification method was applied to ...
Rahma Micho Widyanto +6 more
doaj +1 more source