Results 1 to 10 of about 12,672 (167)
Signal and noise in metabarcoding data.
PLoS ONE, 2023 Metabarcoding is a powerful molecular tool for simultaneously surveying hundreds to thousands of species from a single sample, underpinning microbiome and environmental DNA (eDNA) methods.
Zachary Gold +14 more
doaj +4 more sources
Environmental DNA, 2020 Fine‐scale estimation of trophic interactions is an important subject in the field of ecology. Diet analysis based on fecal DNA metabarcoding has been accepted as a noninvasive, accurate, and time‐ and cost‐effective tool to determine animal diets. Here,
Haruka Mukai +2 more
exaly +2 more sources
Ecological Indicators, 2023 Environmental DNA (eDNA) metabarcoding has been used to study fish diversity in various aquatic ecosystems. However, studies on fish community structure in estuarine ecosystems have not been well corroborated by eDNA metabarcoding, and comparisons with ...
Zuozhi Chen
exaly +3 more sources
PIMBA: A PIpeline for MetaBarcoding Analysis [PDF]
, 2021 ABSTRACT Summary DNA metabarcoding is an emerging monitoring method capable of assessing biodiversity from environmental samples (eDNA). Advances in computational tools have been required due to the increase of Next-Generation Sequencing data.
Renato R. M. Oliveira +3 more
openaire +1 more source
Toward quantitative metabarcoding
Ecology, 2022 Abstract Amplicon-sequence data from environmental DNA (eDNA) and microbiome studies provides important information for ecology, conservation, management, and health. At present, amplicon-sequencing studies – known also as metabarcoding studies, in which the primary data consist of targeted, amplified fragments of DNA sequenced from ...
Andrew Olaf Shelton +10 more
openaire +3 more sources
The power of metabarcoding: Can we improve bioassessment and biodiversity surveys of stream macroinvertebrate communities? [PDF]
Metabarcoding and Metagenomics, 2021 Most stream bioassessment and biodiversity surveys are currently based on morphological identification of communities. However, DNA metabarcoding is emerging as a fast and cost-effective alternative for species identification. We compared both methods in
Jarno Turunen +5 more
doaj +3 more sources
Diversity, 2022 DNA metabarcoding has proven to be an accessible, cost-effective, and non-invasive tool for dietary analysis of predators in situ. Although DNA metabarcoding provides numerous benefits in characterizing diet—such as detecting prey animals that are ...
Adam J. D. Marques +2 more
doaj +1 more source
An Optimized Metabarcoding Method for Mimiviridae [PDF]
Microorganisms, 2020 Mimiviridae is a group of viruses with large genomes and virions. Ecological relevance of Mimiviridae in marine environments has been increasingly recognized through the discoveries of novel isolates and metagenomic studies. To facilitate ecological profiling of Mimiviridae, we previously proposed a meta-barcoding approach based on 82 degenerate primer
Florian Prodinger +12 more
openaire +4 more sources
VTAM: A robust pipeline for validating metabarcoding data using controls
Computational and Structural Biotechnology Journal, 2023 To obtain accurate estimates for biodiversity and ecological studies, metabarcoding studies should be carefully designed to minimize both false positive (FP) and false negative (FN) occurrences.
Aitor González +7 more
doaj +1 more source
Quantification of marine benthic communities with metabarcoding [PDF]
Molecular Ecology Resources, 2021 AbstractDNA metabarcoding methods have been implemented in studies aimed at detecting and quantifying marine benthic biodiversity. In such surveys, universal barcodes are amplified and sequenced from environmental DNA. To quantify biodiversity with DNA metabarcoding, a relation between the number of DNA sequences of a species and its biomass and/or the
Lise Klunder +5 more
openaire +3 more sources