Results 1 to 10 of about 30,204 (224)

Methodological trends and perspectives of animal dietary studies by noninvasive fecal DNA metabarcoding

open access: yesEnvironmental DNA, 2020
Fine‐scale estimation of trophic interactions is an important subject in the field of ecology. Diet analysis based on fecal DNA metabarcoding has been accepted as a noninvasive, accurate, and time‐ and cost‐effective tool to determine animal diets. Here,
Haruka Mukai, Taketo Komura, Haruko Ando
exaly   +2 more sources

Comparison of environmental DNA metabarcoding and bottom trawling for detecting seasonal fish communities and habitat preference in a highly disturbed estuary

open access: yesEcological Indicators, 2023
Environmental DNA (eDNA) metabarcoding has been used to study fish diversity in various aquatic ecosystems. However, studies on fish community structure in estuarine ecosystems have not been well corroborated by eDNA metabarcoding, and comparisons with ...
Zuozhi Chen
exaly   +3 more sources

Introducing a novel 28S rRNA marker for improved assessment of coral reef biodiversity. [PDF]

open access: yesPeerJ
Biodiversity monitoring based on DNA metabarcoding depends on primer performance. Here, we develop a new metabarcoding primer pair that targets a ∼318 bp fragment of the 28S rRNA gene that is highly conserved across branches of the tree of life. We apply
Martineau G   +4 more
europepmc   +3 more sources

The power of metabarcoding: Can we improve bioassessment and biodiversity surveys of stream macroinvertebrate communities? [PDF]

open access: yesMetabarcoding and Metagenomics, 2021
Most stream bioassessment and biodiversity surveys are currently based on morphological identification of communities. However, DNA metabarcoding is emerging as a fast and cost-effective alternative for species identification. We compared both methods in
Jarno Turunen   +5 more
doaj   +3 more sources

COI Metabarcoding Provides Insights into the Highly Diverse Diet of a Generalist Salamander, Salamandra salamandra (Caudata: Salamandridae)

open access: yesDiversity, 2022
DNA metabarcoding has proven to be an accessible, cost-effective, and non-invasive tool for dietary analysis of predators in situ. Although DNA metabarcoding provides numerous benefits in characterizing diet—such as detecting prey animals that are ...
Adam J. D. Marques   +2 more
doaj   +1 more source

Signal and noise in metabarcoding data.

open access: yesPLoS ONE, 2023
Metabarcoding is a powerful molecular tool for simultaneously surveying hundreds to thousands of species from a single sample, underpinning microbiome and environmental DNA (eDNA) methods.
Zachary Gold   +14 more
doaj   +1 more source

VTAM: A robust pipeline for validating metabarcoding data using controls

open access: yesComputational and Structural Biotechnology Journal, 2023
To obtain accurate estimates for biodiversity and ecological studies, metabarcoding studies should be carefully designed to minimize both false positive (FP) and false negative (FN) occurrences.
Aitor González   +7 more
doaj   +1 more source

Diatom metabarcoding and microscopic analyses from sediment samples at Lake Nam Co, Tibet: The effect of sample-size and bioinformatics on the identified communities

open access: yesEcological Indicators, 2021
Diatoms (Bacillariophyceae) are characterized by silicified cell walls that favor their long-term preservation in sediments, therefore widely used as bioindicators of present and past water conditions.
Wengang Kang   +10 more
doaj   +1 more source

Comparison of traditional and DNA metabarcoding samples for monitoring tropical soil arthropods (Formicidae, Collembola and Isoptera)

open access: yesScientific Reports, 2022
The soil fauna of the tropics remains one of the least known components of the biosphere. Long-term monitoring of this fauna is hampered by the lack of taxonomic expertise and funding. These obstacles may potentially be lifted with DNA metabarcoding.
Yves Basset   +23 more
doaj   +1 more source

Internal transcribed spacer primer evaluation for vascular plant metabarcoding [PDF]

open access: yesMetabarcoding and Metagenomics, 2021
The unprecedented ongoing biodiversity decline necessitates scalable means of monitoring in order to fully understand the underlying causes. DNA metabarcoding has the potential to provide a powerful tool for accurate and rapid biodiversity monitoring ...
Andreas Kolter, Birgit Gemeinholzer
doaj   +3 more sources

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