Results 11 to 20 of about 12,672 (167)

Diatom metabarcoding and microscopic analyses from sediment samples at Lake Nam Co, Tibet: The effect of sample-size and bioinformatics on the identified communities

open access: yesEcological Indicators, 2021
Diatoms (Bacillariophyceae) are characterized by silicified cell walls that favor their long-term preservation in sediments, therefore widely used as bioindicators of present and past water conditions.
Wengang Kang   +10 more
doaj   +1 more source

Internal transcribed spacer primer evaluation for vascular plant metabarcoding [PDF]

open access: yesMetabarcoding and Metagenomics, 2021
The unprecedented ongoing biodiversity decline necessitates scalable means of monitoring in order to fully understand the underlying causes. DNA metabarcoding has the potential to provide a powerful tool for accurate and rapid biodiversity monitoring ...
Andreas Kolter, Birgit Gemeinholzer
doaj   +3 more sources

Comparison of traditional and DNA metabarcoding samples for monitoring tropical soil arthropods (Formicidae, Collembola and Isoptera)

open access: yesScientific Reports, 2022
The soil fauna of the tropics remains one of the least known components of the biosphere. Long-term monitoring of this fauna is hampered by the lack of taxonomic expertise and funding. These obstacles may potentially be lifted with DNA metabarcoding.
Yves Basset   +23 more
doaj   +1 more source

An Illumina metabarcoding pipeline for fungi [PDF]

open access: yesEcology and Evolution, 2014
AbstractHigh‐throughput metabarcoding studies on fungi and other eukaryotic microorganisms are rapidly becoming more frequent and more complex, requiring researchers to handle ever increasing amounts of raw sequence data. Here, we provide a flexible pipeline for pruning and analyzing fungal barcode (ITS rDNA) data generated as paired‐end reads on ...
Bálint, Miklós   +4 more
openaire   +3 more sources

Diversity and ecology of protists revealed by metabarcoding [PDF]

open access: yesCurrent Biology, 2021
Protists are the dominant eukaryotes in the biosphere where they play key functional roles. While protists have been studied for over a century, it is the high-throughput sequencing of molecular markers from environmental samples - the approach of metabarcoding - that has revealed just how diverse, and abundant, these small organisms are. Metabarcoding
Burki F, Sandin MM, Jamy M
openaire   +4 more sources

Comparison of mechanical sorting and DNA metabarcoding for diet analysis with fresh and degraded wolf scats

open access: yesEcosphere, 2021
DNA metabarcoding has become a powerful technique for identifying the species present in a bulk environmental sample. The application of DNA metabarcoding to wildlife diet analysis is a particularly promising tool for exploring trophic interactions.
Aimee L. Massey   +4 more
doaj   +1 more source

The problem of omnivory: A synthesis on omnivory and DNA metabarcoding [PDF]

open access: yesMolecular Ecology, 2021
AbstractDietary analysis using DNA metabarcoding is a powerful tool that is increasingly being used to further our knowledge of trophic interactions in highly complex food webs but is not without limitations. Omnivores, the most generalist of consumers, pose unique challenges when using such methods.
Maximillian P. T. G. Tercel   +2 more
openaire   +4 more sources

DNA metabarcoding quantifies the relative biomass of arthropod taxa in songbird diets: Validation with camera‐recorded diets

open access: yesEcology and Evolution, 2022
Ecological research is often hampered by the inability to quantify animal diets. Diet composition can be tracked through DNA metabarcoding of fecal samples, but whether (complex) diets can be quantitatively determined with metabarcoding is still debated ...
Yvonne I. Verkuil   +8 more
doaj   +1 more source

Persisting roadblocks in arthropod monitoring using non-destructive metabarcoding from collection media of passive traps [PDF]

open access: yesPeerJ, 2023
Background Broad-scale monitoring of arthropods is often carried out with passive traps (e.g., Malaise traps) that can collect thousands of specimens per sample.
Lucas Sire   +11 more
doaj   +2 more sources

Proper environmental DNA metabarcoding data transformation reveals temporal stability of fish communities in a dendritic river system

open access: yesEnvironmental DNA, 2021
Protecting freshwater biodiversity is considered an ultimate challenge but depends on reliable surveys of species distribution and abundance which eDNA metabarcoding (environmental DNA metabarcoding) may offer.
Martin Laporte   +8 more
doaj   +1 more source

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