Results 31 to 40 of about 12,672 (167)
Frontiers in Ecology and Evolution, 2019 Metabarcoding is rapidly gaining popularity as a means of conducting biodiversity studies. Using DNA barcodes to identify and catalog biodiversity has many advantages, and compares favorably with traditional methods based on morphological examination ...
Mac P. Pierce
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Observation Bias in Metabarcoding
Molecular Ecology ResourcesABSTRACT DNA metabarcoding is subject to observation bias associated with PCR and sequencing, which can result in observed read proportions differing from actual species proportions in the DNA extract. Here, we amplify and sequence a mock community of known composition containing marine fishes and cetaceans using four different primer
Megan R. Shaffer +5 more
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Environmental DNA, 2021 Increasing evidence for global insect declines is prompting a renewed interest in the survey of whole insect communities. DNA metabarcoding can contribute to assessing diverse insect communities over a range of spatial and temporal scales, but efforts ...
Vanessa A. Mata +6 more
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Environmental MicrobiomeBackground Culturing of fungi is labor-intensive and reveals limited diversity, while high-throughput sequencing of barcodes (i.e., metabarcoding) enables a simultaneous detection of fungi from multiple environmental samples.
Jana Mittelstrass +7 more
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Foods, 2021 The substitution of more appreciated animal species by animal species of lower commercial value is a common type of meat product adulteration. DNA metabarcoding, the combination of DNA barcoding with next-generation sequencing (NGS), plays an increasing ...
Laura Preckel +6 more
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DNA metabarcoding reveals greater plant diversity than morphological seed analysis of bird feces
Applications in Plant SciencesPremise Fruit‐eating birds drive seed dispersal in recovering tropical ecosystems, shaping forest regeneration. Molecular techniques, such as DNA metabarcoding, enable diet analysis from feces and can provide complementary frugivory data where dispersal ...
Carina I. Motta +3 more
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Metabarcoding From Microbes to Mammals: Comprehensive Bioassessment on a Global Scale
Frontiers in Ecology and Evolution, 2020 Global biodiversity loss is unprecedented, and threats to existing biodiversity are growing. Given pervasive global change, a major challenge facing resource managers is a lack of scalable tools to rapidly and consistently measure Earth's biodiversity ...
Zacchaeus G. Compson +5 more
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Comparison and validation of some ITS primer pairs useful for fungal metabarcoding studies.
PLoS ONE, 2014 Current metabarcoding studies aiming to characterize microbial communities generally rely on the amplification and sequencing of relatively short DNA regions.
Michiel Op De Beeck +5 more
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DNA Metabarcoding of Amazonian Ichthyoplankton Swarms
PLOS ONE, 2017 Les forêts tropicales humides abritent une biodiversité extraordinaire. On pense que le bassin amazonien abrite 30 % de toutes les espèces de poissons de rivière dans le monde. Les informations sur l'écologie, la reproduction et le recrutement de la plupart des espèces font toujours défaut, ce qui entrave la gestion des pêches et le succès des ...
Marie-Eugénie Maggia +10 more
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Environmental DNA Metabarcoding Detects Predators at Higher Rates Than Electrofishing
Environmental DNAThere are numerous downsides and risks associated with electrofishing; hence, environmental DNA (eDNA) metabarcoding is becoming increasingly common in aquatic ecological studies.
Eric A. Bonk +3 more
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