Results 31 to 40 of about 24,742 (210)

Endolichenic Fungal Community Analysis by Pure Culture Isolation and Metabarcoding: A Case Study of Parmotrema tinctorum

open access: yesMycobiology, 2022
Lichen is a symbiotic mutualism of mycobiont and photobiont that harbors diverse organisms including endolichenic fungi (ELF). Despite the taxonomic and ecological significance of ELF, no comparative investigation of an ELF community involving isolation ...
Ji Ho Yang   +3 more
doaj   +1 more source

Minimizing polymerase biases in metabarcoding

open access: yesMolecular Ecology Resources, 2018
AbstractDNA metabarcoding is an increasingly popular method to characterize and quantify biodiversity in environmental samples. Metabarcoding approaches simultaneously amplify a short, variable genomic region, or “barcode,” from a broad taxonomic group via the polymerase chain reaction (PCR), using universal primers that anneal to flanking conserved ...
Nichols, Ruth V.   +7 more
openaire   +4 more sources

Periphyton diversity in two different Antarctic lakes assessed using metabarcoding

open access: yes, 2021
Antarctic lakes have generally simple periphyton communities when compared with those of lower latitudes. To date, assessment of microbial diversity in Antarctica has relied heavily on traditional direct observation and cultivation methods. In this study,
Rosa, Luiz Henrique   +9 more
core   +1 more source

An assessment of minimum sequence copy thresholds for identifying and reducing the prevalence of artefacts in dietary metabarcoding data [PDF]

open access: yes, 2021
Metabarcoding provides a powerful tool for investigating biodiversity and trophic interactions, but the high sensitivity of this methodology makes it vulnerable to errors, resulting in artefacts in the final data. Metabarcoding studies thus often utilise
Drake, Lorna E.   +17 more
core   +1 more source

Aerobiological Monitoring and Metabarcoding of Grass Pollen

open access: yesPlants, 2023
Grass pollen is one of the leading causes of pollinosis, affecting 10–30% of the world’s population. The allergenicity of pollen from different Poaceae species is not the same and is estimated from moderate to high. Aerobiological monitoring is a standard method that allows one to track and predict the dynamics of allergen concentration in the air ...
Anastasia A. Krinitsina   +8 more
openaire   +3 more sources

Calibrating Environmental DNA Metabarcoding to Conventional Surveys for Measuring Fish Species Richness

open access: yesFrontiers in Ecology and Evolution, 2020
The ability to properly identify species present in a landscape is foundational to ecology and essential for natural resource management and conservation.
Mary E. McElroy   +19 more
doaj   +1 more source

Ichthyoplankton DNA metabarcoding: Challenges and perspectives [PDF]

open access: yesMolecular Ecology, 2021
DNA metabarcoding has been widely used to access and monitor species. However, several challenges remain open for its mainstream application in ecological studies, particularly when dealing with a quantitative approach. In a From the Cover article in this issue of Molecular Ecology, Mariac et al. (2021) report species‐level ichthyoplankton dynamics for
openaire   +2 more sources

Filling in the Gaps: Adopting Ultraconserved Elements Alongside COI to Strengthen Metabarcoding Studies

open access: yesFrontiers in Ecology and Evolution, 2019
Metabarcoding is rapidly gaining popularity as a means of conducting biodiversity studies. Using DNA barcodes to identify and catalog biodiversity has many advantages, and compares favorably with traditional methods based on morphological examination ...
Mac P. Pierce
doaj   +1 more source

A manager’s guide to using eDNA metabarcoding in marine ecosystems [PDF]

open access: yesPeerJ, 2022
Environmental DNA (eDNA) metabarcoding is a powerful tool that can enhance marine ecosystem/biodiversity monitoring programs. Here we outline five important steps managers and researchers should consider when developing eDNA monitoring program: (1 ...
Zachary Gold   +11 more
doaj   +2 more sources

Carpophilus Metabarcoding

open access: yes, 2021
Data to support the in-progress paper https://github.com/alexpiper ...
openaire   +1 more source

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