Results 51 to 60 of about 12,672 (167)
, 2022 The following steps include the protocols of the 1. Diet Regime 2. Dissection procedure 3. Beads extraction 4. Salt-out DNA extraction
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Environmental DNA, 2020 Environmental DNA metabarcoding has been widely touted as a powerful tool for monitoring biodiversity in marine ecosystems. However, this method still requires thorough validation and standardization before it can be widely applied for ecological ...
Kevin C. Morey +2 more
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Characterizing parasitic nematode faunas in faeces and soil using DNA metabarcoding
Parasites & Vectors, 2021 Background Gastrointestinal parasitic nematodes can impact fecundity, development, behaviour, and survival in wild vertebrate populations. Conventional monitoring of gastrointestinal parasitic nematodes in wild populations involves morphological ...
Marie Louise Davey +2 more
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How quantitative is metabarcoding: A meta‐analytical approach [PDF]
Molecular Ecology, 2018 AbstractMetabarcoding has been used in a range of ecological applications such as taxonomic assignment, dietary analysis and the analysis of environmental DNA. However, after a decade of use in these applications there is little consensus on the extent to which proportions of reads generated corresponds to the original proportions of species in a ...
Philip D. Lamb +5 more
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Comparative analysis of eDNA metabarcoding and eDNA metagenomics for fish biodiversity estimates using standard and novel filtration methods [PDF]
Metabarcoding and MetagenomicsMolecular techniques involving environmental DNA (eDNA) are increasingly used for aquatic species detection. Metabarcoding, a widely adopted technique, suffers from primer bias: uneven amplification of species due to primer mismatches. Primer bias can be
Daniël van Berkel +4 more
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Comparison of Metabarcoding Techniques for Dietary Assessment in Herbivores and Omnivores [PDF]
BIO Web of ConferencesDietary assessment plays a crucial role in comprehending the ecological dynamics and nutritional needs of herbivores and omnivores. The metabarcoding technique has emerged as a potent tool for exploring the dietary composition of these animals.
Hartini Sariyati Nur +10 more
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DBP Metabarcoding Pipeline (for metabarcoding data using nanopore) v1
This pipeline was developed to process MinION nanopore data output for metabarcoding purposes. The process begins with raw FASTQ data from nanopore base-calling output and includes several steps: quality filtering with NanoFilt, primer removal and length trimming with Cutadapt, artifact detection with Chimera, and building an Operational Taxonomic Unit
Muhammad Danie Al Malik +2 more
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Efficacy of metabarcoding for identification of fish eggs evaluated with mock communities
Ecology and Evolution, 2020 There is urgent need for effective and efficient monitoring of marine fish populations. Monitoring eggs and larval fish may be more informative than that traditional fish surveys since ichthyoplankton surveys reveal the reproductive activities of fish ...
Elena M. Duke, Ronald S. Burton
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Ecological IndicatorsAlthough environmental DNA (eDNA) metabarcoding technology has been increasingly applied in freshwater ecosystems, the effectiveness of conventional water sampling and other adaptive metabarcoding methods for detecting macroinvertebrates has not been ...
Kena Shi +5 more
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Ecological IndicatorsMangrove ecosystems, located at the vital interface between land and sea, exhibit exceptionally high biodiversity. Meiofauna are key components in the food webs and nutrient cycles of mangrove ecosystems. However, the diversity of intertidal meiofauna is
Mingcheng Hu +6 more
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