Metabarcoding and eDNA for insect conservation
Environmental DNA (eDNA) samples and metabarcoding studies have emerged as transformative sampling methods and techniques with potential in the field of insect conservation. eDNA refers to genetic material shed by organisms into their environment. By extracting and sequencing this DNA, it is possible to detect the presence of target species without the
Bierman, Anandi, Lloyd, Melissa
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Comparison between COI Metabarcoding and Reverse Metabarcoding with Nanopore’s MinION
Metabarcoding have emerged as a vital tool for assessing biodiversity and early detecting invasive species. It is a technique where a certain marker gene, or barcode, is amplified from a DNA sample, sequenced and subsequently classified by comparisons to known reference genomes.
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Integrating Megabarcoding and Metabarcoding to Unlock Diversity and Distribution Data Shortfalls in Dark Taxa. [PDF]
Mol Ecol ResourSantos-Perdomo I +12 more
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Comparing DNA metabarcoding with light microscopy to identify eukaryotic phytoplankton in the Baltic Sea, Kattegat and Skagerrak. [PDF]
Sci RepTorstensson A +9 more
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pr2-Wormifier: A Bioinformatics Pipeline to Create Custom Reference Databases for Improved Metabarcoding of Marine Protists. [PDF]
Mol Ecol ResourKnell S, Romahn J, Bálint M.
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Standardizing food web reconstruction from metabarcoding data using iterative rarefaction and trait-based trophic inference model. [PDF]
MethodsXLecegui V, Jassey VEJ, Pérez-Haase A.
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Phytoplankton Metabarcoding Data Processing
Phytoplankton taxonomy was undertaken using DNA metabarcoding (along with traditional taxonomic approaches via microscopy). The filter samples were extracted for DNA using E.Z.N.A. water DNA kits using the default protocol (Omega Bio-Tek, USA). After extraction, the samples were PCR amplified using the 18s rRNA V9 region with unique dual-tags using the
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Comparative Analysis of Lepidopteran Community Structure Using DNA Metabarcoding: Warm-Temperate Forest Versus Grass-Shrub Ecotones in Pangquangou National Nature Reserve. [PDF]
Ecol EvolWu L +9 more
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Invisible invaders: A new warning flag for molecularly detected alien species (MODAS) in databases and information systems. [PDF]
Ecol ApplGuy-Haim T +4 more
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