Results 71 to 80 of about 37,918 (308)

Comparative analysis of fungal microbiomes in Korean and Chinese chili pepper powders

Journal of the Science of Food and Agriculture, EarlyView.
Abstract BACKGROUND Chili pepper powder plays a crucial role in kimchi fermentation; however, its use may result in fungal contamination. Therefore, analyzing its mycobiome is vital to maintain quality control standards. In this study, we used internal transcribed spacer (ITS)2 amplicon sequencing to compare fungal communities in commercially available
Jin‐Hee Seo, Ji‐Hyoung Ha, Su‐Hyeon Cho, Kil‐Nam Kim, Hae‐Won Lee   +4 more
wiley   +1 more source

Improving plant DNA metabarcoding accuracy with ecological filters and Angiosperms353: Field and pollen microscopy validation

Applications in Plant Sciences
Premise Metabarcoding has become a successful tool for the identification of species in ecological assemblages. However, the usefulness of metabarcoding for identifying plant species has been hampered due to a lack of universal gene regions that work ...
Reed Clark Benkendorf, Emily J. Woodworth, Paul J. CaraDonna, Jane E. Ogilvie, Sophie Taddeo, Jeremie B. Fant   +5 more
doaj   +1 more source

Exploring within-host diversity of plant viruses in rice agrosystems using two high-throughput sequencing strategies [PDF]

, 2018
The virome is an essential component of the phytobiome as viruses correspond to the most abundant and diverse biological entity in Nature and interact with all living organisms. The recent advances in high-throughput sequencing (HTS) has proven efficient
Dereeper, Alexis, Fargette, Denis, Fernandez, Emmanuel, Filloux, Denis, Hebrard, Eugénie, Hubert, Judith, Julian, Charlotte, Pinel-Galzi, Agnès, Poulicard, Nils, Roumagnac, Philippe   +9 more
core  

Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples [PDF]

, 2018
Advances in the sequencing of DNA extracted from media such as soil and water offer huge opportunities for biodiversity monitoring and assessment, particularly where the collection or identification of whole organisms is impractical.
Banks, Jonathan C., Boyer, Stephane, Buckley, Hannah L., Buckley, Thomas R., Cruickshank, Rob, Dickie, Ian, Dopheide, Andrew, Handley, Kim M., Hermans, Syrie, Holdaway, Robert, Kamke, Janine, Lear, Gavin, Lee, Charles Kai-Wu, MacDiarmid, Robin, Morales, Sergio E., Orlovich, David A., Smissen, Rob, Wood, Jamie   +17 more
core   +3 more sources

Underground Lag: Fungal Community and Edaphic Legacies After Disturbance

Land Degradation &Development, EarlyView.
ABSTRACT Páramos are neotropical mountain ecosystems that regulate water and store large amounts of carbon, but are increasingly degraded by agriculture and grazing. Although native vegetation often recolonizes after abandonment, belowground recovery remains poorly understood.
Wilmer Dajhan Navarrete‐López, Arantzazu Molins, María Elena Solarte, Camila Enriquez‐Paz, Joshua Borràs, Josefina Bota, Elena Baraza   +6 more
wiley   +1 more source

Laying Down the Foundations for Qualitative and Quantitative Freshwater Zooplankton Metabarcoding Surveys

Environmental DNA
Metabarcoding has been proven to be a highly effective tool for surveying biodiversity in aquatic ecosystems. Despite the recent explosion of metabarcoding studies, relatively few efforts have focused on freshwater zooplankton communities, even though ...
Jonas Bylemans, Didier Azam, Anne‐Laure Besnard, Simon Blanchet, Cecile Chardon, Gérard Lacroix, Leslie Laine, Stephen Mulero, Carine Puppo, Murielle Richard, Marine Vautier, Isabelle Domaizon   +11 more
doaj   +1 more source

Guidance and best practices for species identification using eDNA metabarcoding – When do you call a cod a cod? [PDF]

Metabarcoding and Metagenomics
With the rapid uptake of environmental DNA (eDNA) metabarcoding for a wide range of conservation and management uses, there is a growing need for guidance and best practices for species identification. A range of methodological and interpretive decisions
Alison W. Watts, Zachary Gold, Nastassia V. Patin, Nicolaus G. Adams, Jacoby D. Baker, Fouad El Baidouri, Erin Grey, Ann E. Holmes, Kym Jacobson, Sean Jungbluth, Tadashi Kajita, Anders Kiledal, Matthew A. Lemay, Enrique Montes, Frank E. Muller-Karger, Jeffrey Miller, Matthew B. Ogburn, Kathleen J. Pitz, Katherine Silliman, Andrew Thompson, Luke R. Thompson   +20 more
doaj   +3 more sources

Comparison between fluorometry and microscopy‐based phytoplankton assessments in the Laurentian Great Lakes

Limnology and Oceanography: Methods, EarlyView.
Abstract Photosynthetic pigment fluorescence is commonly used in limnology and oceanography as a proxy for phytoplankton biomass. Fluorometry has been used to detect subsurface algal blooms, characterize dynamics of the deep chlorophyll layer, and to provide greater vertical resolution to phytoplankton monitoring.
Katya E. Kovalenko, Euan D. Reavie, Christopher T. Filstrup, Anne Scofield, Meaghan Lightfoot, Erica Yang, Elizabeth E. Alexson   +6 more
wiley   +1 more source

Transforming gastrointestinal helminth parasite identification in vertebrate hosts with metabarcoding: a systematic review

Parasites & Vectors
Background Gastrointestinal helminths are a very widespread group of intestinal parasites that can cause major health issues in their hosts, including severe illness or death.
Madison L. Miller, Christopher Rota, Amy Welsh   +2 more
doaj   +1 more source

Sexual reproduction in invasive Japanese knotweed (Reynoutria japonica) in south Sweden

Nordic Journal of Botany, EarlyView.
To understand the potential for northward expansion of knotweeds into currently uninvaded regions, it is crucial to assess the potential for sexual reproduction alongside the ongoing vegetative spread. For that reason, we tested viability in Reynoutria japonica seeds in south Sweden, a region where viable seeds had not been previously reported ...
Tina D'Hertefeldt, Annette Andersson, Ellen Andersson, Bea Nerer, Mattias West, Alexander Zimmerman   +5 more
wiley   +1 more source