Results 61 to 70 of about 30,204 (224)

Can DNA from wet, temperate soils be used for palaeoecological reconstruction?

Journal of Quaternary Science, EarlyView.
ABSTRACT Sedimentary ancient DNA in well‐dated contexts (sedaDNA) has great potential as a palaeoecological proxy. A few studies have attempted palaeoecological reconstructions using sedaDNA from soil; these studies have been mainly restricted to cold and/or dry soils.
Nick A. Cutler, Doreen Yu‐Tuan Huang, Richard T. Streeter, Andrew J. Dugmore   +3 more
wiley   +1 more source

Laying Down the Foundations for Qualitative and Quantitative Freshwater Zooplankton Metabarcoding Surveys

Environmental DNA
Metabarcoding has been proven to be a highly effective tool for surveying biodiversity in aquatic ecosystems. Despite the recent explosion of metabarcoding studies, relatively few efforts have focused on freshwater zooplankton communities, even though ...
Jonas Bylemans, Didier Azam, Anne‐Laure Besnard, Simon Blanchet, Cecile Chardon, Gérard Lacroix, Leslie Laine, Stephen Mulero, Carine Puppo, Murielle Richard, Marine Vautier, Isabelle Domaizon   +11 more
doaj   +1 more source

Molecular metrics to monitor ecological status of large rivers: Implementation of diatom DNA metabarcoding in the Joint Danube Survey 4

Ecological Indicators
The Joint Danube Survey (JDS) is a regular transnational survey to monitor the quality of the Danube and its main tributaries, in accordance with the EU Water Framework Directive.
Kálmán Tapolczai, Teofana Chonova, Dana Fidlerová, Jarmila Makovinská, Demetrio Mora, Alexander Weigand, Jonas Zimmermann   +6 more
doaj   +1 more source

The applicability of eDNA metabarcoding approaches for sessile benthic surveying in the Kimberley region, north‐western Australia

Environmental DNA, 2022
The application of environmental DNA technologies is a promising new approach to rapidly audit biodiversity across large‐scale, remote regions. Here, we examine the efficacy of a dual‐assay eDNA metabarcoding approach for sessile benthic bioassessments ...
Katrina M. West, Arne A. S. Adam, Nicole White, William D. Robbins, Daniel Barrow, Adrian Lane, Zoe T.Richards   +6 more
doaj   +1 more source

Holocene vegetation dynamics of Grande Terre (Kerguelen Islands) inferred from sedimentary ancient DNA

Journal of Quaternary Science, EarlyView.
ABSTRACT While the Antarctic region has experienced significant climatic changes over the Holocene, few high‐resolution records have examined their effects on the local biota. In order to understand past ecological shifts and forecast future changes, it is important to obtain high‐resolution palaeoecological records from this region.
Xaali O'Reilly‐Berkeley, Aloïs Revéret, Youri Lammers, Maaike Zwier, Anne E. Bjune, Fabien Arnaud, Jostein Bakke, Sandra Nogué, Inger G. Alsos   +8 more
wiley   +1 more source

Is DNA metabarcoding an option for formaldehyde‐preserved zooplankton time series?

Limnology and Oceanography: Methods, EarlyView.
Abstract The recovery of amplifiable DNA from formaldehyde‐fixed (FF) zooplankton samples has long been considered problematic, but advances in degraded‐DNA retrieval have renewed interest in FF samples. To access the information stored in long‐term zooplankton time series, we evaluated methods for extracting amplifiable DNA from community samples ...
Aitor Albaina, Anders Lanzén, Irati Miguel, Fernando Rendo, María Santos   +4 more
wiley   +1 more source

Technical challenges in the implementation of macroinvertebrate DNA metabarcoding in river biomonitoring: A case study from peninsular Spain—Advantages, limitations and roadmap

Ecological Solutions and Evidence
European Union member states are required to monitor the ecological status of their water bodies. The existing official protocols and indices for routine biomonitoring are usually based on the morphological identification of different groups of indicator
Álvaro Fueyo, Omar Sánchez, Carlos Carleos, Javier Granero‐Castro, Yaisel J. Borrell   +4 more
doaj   +1 more source

Validating environmental DNA metabarcoding for marine fishes in diverse ecosystems using a public aquarium

Environmental DNA, 2020
Environmental DNA metabarcoding has been widely touted as a powerful tool for monitoring biodiversity in marine ecosystems. However, this method still requires thorough validation and standardization before it can be widely applied for ecological ...
Kevin C. Morey, Timothy J. Bartley, Robert H. Hanner   +2 more
doaj   +1 more source

Protocolo para toma de muestras ambientales de agua, suelo y sedimento y extracción de ADN para metabarcoding

, 2020
Presentación realizada en el Curso de capacitación teórico - demostrativo - "Monitoreo de la biodiversidad con metabarcoding" organizado por el Instituto Humboldt los días 24 al 27 de noviembre del año 2020.

core  

16S metabarcoding tutorial

, 2022
Tutorial for the analysis of 16S rRNA metabarcoding sequences using the MiSeq SOP pipeline.
Cumsille, Andrés
core   +1 more source