Results 41 to 50 of about 12,672 (167)

Plant biodiversity assessment through pollen DNA metabarcoding in Natura 2000 habitats (Italian Alps)

Scientific Reports, 2021
Monitoring biodiversity is of increasing importance in natural ecosystems. Metabarcoding can be used as a powerful molecular tool to complement traditional biodiversity monitoring, as total environmental DNA can be analyzed from complex samples ...
Kleopatra Leontidou, Despoina Vokou, Anna Sandionigi, Antonia Bruno, Maria Lazarina, Johannes De Groeve, Mingai Li, Claudio Varotto, Matteo Girardi, Maurizio Casiraghi, Antonella Cristofori   +10 more
doaj   +1 more source

Guidance and best practices for species identification using eDNA metabarcoding – When do you call a cod a cod? [PDF]

Metabarcoding and Metagenomics
With the rapid uptake of environmental DNA (eDNA) metabarcoding for a wide range of conservation and management uses, there is a growing need for guidance and best practices for species identification. A range of methodological and interpretive decisions
Alison W. Watts, Zachary Gold, Nastassia V. Patin, Nicolaus G. Adams, Jacoby D. Baker, Fouad El Baidouri, Erin Grey, Ann E. Holmes, Kym Jacobson, Sean Jungbluth, Tadashi Kajita, Anders Kiledal, Matthew A. Lemay, Enrique Montes, Frank E. Muller-Karger, Jeffrey Miller, Matthew B. Ogburn, Kathleen J. Pitz, Katherine Silliman, Andrew Thompson, Luke R. Thompson   +20 more
doaj   +3 more sources

Comparative environmental RNA and DNA metabarcoding analysis of river algae and arthropods for ecological surveys and water quality assessment

Scientific Reports, 2022
Environmental DNA (eDNA) metabarcoding is widely used for species analysis, while the use of environmental RNA (eRNA) metabarcoding is more limited. We conducted comparative eDNA/eRNA metabarcoding of the algae and arthropods (aquatic insects) in water ...
Kaede Miyata, Yasuaki Inoue, Yuto Amano, Tohru Nishioka, Tomohisa Nagaike, Takamitsu Kawaguchi, Osamu Morita, Masayuki Yamane, Hiroshi Honda   +8 more
doaj   +1 more source

Transforming gastrointestinal helminth parasite identification in vertebrate hosts with metabarcoding: a systematic review

Parasites & Vectors
Background Gastrointestinal helminths are a very widespread group of intestinal parasites that can cause major health issues in their hosts, including severe illness or death.
Madison L. Miller, Christopher Rota, Amy Welsh   +2 more
doaj   +1 more source

Improving plant DNA metabarcoding accuracy with ecological filters and Angiosperms353: Field and pollen microscopy validation

Applications in Plant Sciences
Premise Metabarcoding has become a successful tool for the identification of species in ecological assemblages. However, the usefulness of metabarcoding for identifying plant species has been hampered due to a lack of universal gene regions that work ...
Reed Clark Benkendorf, Emily J. Woodworth, Paul J. CaraDonna, Jane E. Ogilvie, Sophie Taddeo, Jeremie B. Fant   +5 more
doaj   +1 more source

Laying Down the Foundations for Qualitative and Quantitative Freshwater Zooplankton Metabarcoding Surveys

Environmental DNA
Metabarcoding has been proven to be a highly effective tool for surveying biodiversity in aquatic ecosystems. Despite the recent explosion of metabarcoding studies, relatively few efforts have focused on freshwater zooplankton communities, even though ...
Jonas Bylemans, Didier Azam, Anne‐Laure Besnard, Simon Blanchet, Cecile Chardon, Gérard Lacroix, Leslie Laine, Stephen Mulero, Carine Puppo, Murielle Richard, Marine Vautier, Isabelle Domaizon   +11 more
doaj   +1 more source

Molecular metrics to monitor ecological status of large rivers: Implementation of diatom DNA metabarcoding in the Joint Danube Survey 4

Ecological Indicators
The Joint Danube Survey (JDS) is a regular transnational survey to monitor the quality of the Danube and its main tributaries, in accordance with the EU Water Framework Directive.
Kálmán Tapolczai, Teofana Chonova, Dana Fidlerová, Jarmila Makovinská, Demetrio Mora, Alexander Weigand, Jonas Zimmermann   +6 more
doaj   +1 more source

The applicability of eDNA metabarcoding approaches for sessile benthic surveying in the Kimberley region, north‐western Australia

Environmental DNA, 2022
The application of environmental DNA technologies is a promising new approach to rapidly audit biodiversity across large‐scale, remote regions. Here, we examine the efficacy of a dual‐assay eDNA metabarcoding approach for sessile benthic bioassessments ...
Katrina M. West, Arne A. S. Adam, Nicole White, William D. Robbins, Daniel Barrow, Adrian Lane, Zoe T.Richards   +6 more
doaj   +1 more source

Metabarcoding a diverse arthropod mock community

Molecular Ecology Resources, 2019
Abstract Although DNA metabarcoding is an attractive approach for monitoring biodiversity, it is often difficult to detect all the species present in a bulk sample. In particular, sequence recovery for a given species depends on its biomass and mitome copy number as well as the primer set employed for PCR.
Thomas W. A. Braukmann, Natalia V. Ivanova, Sean W. J. Prosser, Vasco Elbrecht, Dirk Steinke, Sujeevan Ratnasingham, Jeremy R. de Waard, Jayme E. Sones, Evgeny V. Zakharov, Paul D. N. Hebert   +9 more
openaire   +2 more sources

Technical challenges in the implementation of macroinvertebrate DNA metabarcoding in river biomonitoring: A case study from peninsular Spain—Advantages, limitations and roadmap

Ecological Solutions and Evidence
European Union member states are required to monitor the ecological status of their water bodies. The existing official protocols and indices for routine biomonitoring are usually based on the morphological identification of different groups of indicator
Álvaro Fueyo, Omar Sánchez, Carlos Carleos, Javier Granero‐Castro, Yaisel J. Borrell   +4 more
doaj   +1 more source