Results 41 to 50 of about 24,742 (210)

Metabarcoding is powerful yet still blind : a comparative analysis of morphological and molecular surveys of seagrass communities

open access: yes, 2015
In the context of the sixth wave of extinction, reliable surveys of biodiversity are increasingly needed to infer the cause and consequences of species and community declines, identify early warning indicators of tipping points, and provide reliable ...
Grall, J.   +27 more
core   +1 more source

Zooplankton Community Profiling in a Eutrophic Freshwater Ecosystem-Lake Tai Basin by DNA Metabarcoding

open access: yesScientific Reports, 2017
Communities of zooplankton, a critical portion of aquatic ecosystems, can be adversely affected by contamination resulting from human activities. Understanding the influence of environmental change on zooplankton communities under field-conditions is ...
Jianghua Yang   +6 more
doaj   +1 more source

Observation Bias in Metabarcoding

open access: yesMolecular Ecology Resources
ABSTRACT DNA metabarcoding is subject to observation bias associated with PCR and sequencing, which can result in observed read proportions differing from actual species proportions in the DNA extract. Here, we amplify and sequence a mock community of known composition containing marine fishes and cetaceans using four different primer
Megan R. Shaffer   +5 more
openaire   +2 more sources

16S metabarcoding tutorial

open access: yes, 2022
Tutorial for the analysis of 16S rRNA metabarcoding sequences using the MiSeq SOP pipeline.
Cumsille, Andrés
core   +1 more source

Efficient assessment of nocturnal flying insect communities by combining automatic light traps and DNA metabarcoding

open access: yesEnvironmental DNA, 2021
Increasing evidence for global insect declines is prompting a renewed interest in the survey of whole insect communities. DNA metabarcoding can contribute to assessing diverse insect communities over a range of spatial and temporal scales, but efforts ...
Vanessa A. Mata   +6 more
doaj   +1 more source

Metabarcoding with Illumina and Oxford Nanopore Technologies provides complementary insights into tree seed mycobiota

open access: yesEnvironmental Microbiome
Background Culturing of fungi is labor-intensive and reveals limited diversity, while high-throughput sequencing of barcodes (i.e., metabarcoding) enables a simultaneous detection of fungi from multiple environmental samples.
Jana Mittelstrass   +7 more
doaj   +1 more source

Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding

open access: yesFoods, 2021
The substitution of more appreciated animal species by animal species of lower commercial value is a common type of meat product adulteration. DNA metabarcoding, the combination of DNA barcoding with next-generation sequencing (NGS), plays an increasing ...
Laura Preckel   +6 more
doaj   +1 more source

DNA metabarcoding reveals greater plant diversity than morphological seed analysis of bird feces

open access: yesApplications in Plant Sciences
Premise Fruit‐eating birds drive seed dispersal in recovering tropical ecosystems, shaping forest regeneration. Molecular techniques, such as DNA metabarcoding, enable diet analysis from feces and can provide complementary frugivory data where dispersal ...
Carina I. Motta   +3 more
doaj   +1 more source

Metabarcoding From Microbes to Mammals: Comprehensive Bioassessment on a Global Scale

open access: yesFrontiers in Ecology and Evolution, 2020
Global biodiversity loss is unprecedented, and threats to existing biodiversity are growing. Given pervasive global change, a major challenge facing resource managers is a lack of scalable tools to rapidly and consistently measure Earth's biodiversity ...
Zacchaeus G. Compson   +5 more
doaj   +1 more source

Environmental DNA Metabarcoding Detects Predators at Higher Rates Than Electrofishing

open access: yesEnvironmental DNA
There are numerous downsides and risks associated with electrofishing; hence, environmental DNA (eDNA) metabarcoding is becoming increasingly common in aquatic ecological studies.
Eric A. Bonk   +3 more
doaj   +1 more source

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